ABTL-0812 (Synonyms: α-Hydroxylinoleic acid)

Cat. No.: HY-U00141 Purity: ≥98.0%: FAQs
Data Sheet SDS COA Handling Instructions

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ABTL-0812 (α-Hydroxylinoleic acid) induces endoplasmic reticulum (ER) stress-mediated autophagy. ABTL-0812 is a first-in-class small molecule with anti-cancer activity.

For research use only. We do not sell to patients.

ABTL-0812 Chemical Structure

CAS No. : 57818-44-7

Size	Price	Stock	Quantity
5 mg	USD 375	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 635	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 1900	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 2950	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Description

ABTL-0812 (α-Hydroxylinoleic acid) induces endoplasmic reticulum (ER) stress-mediated autophagy. ABTL-0812 is a first-in-class small molecule with anti-cancer activity^[1].

In Vitro

ABTL-0812 (ABTL0812; 10-100 μM; 48 hours) inhibits cell viability of squamous NSCLC H157 cells^[1].
Compared with squamous NSCLC H157 cells, human lung fibroblast cell line MRC-5 are resistant to ABTL0812 treatment^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	Human lung fibroblast MRC5 and squamous non-small cell lung (NSCLC) H157 cells
Concentration:	10, 30, 100 μM
Incubation Time:	48 hours
Result:	Inhibited cell viability of squamous NSCLC H157 cells, not lung fibroblast cell line MRC-5.

In Vivo

ABTL-0812 (ABTL0812; 120 mg/kg; oral gavage; 5 times per week; for 33 d) induces ER stress in human lung and pancreatic xenografts^[1].
ABTL-0812 induces hallmarks of ER stress in vivo. ABTL-0812 increases ATF4 and HSPA5 expression in mice bearing MiaPaca2 and A549 xenograft, respectively^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Athymic female nude mice bearing MiaPaca2 and A549 xenograft models^[1]
Dosage:	120 mg/kg
Administration:	Administered by oral gavage; 5 times per week for 33 days
Result:	Induced ER stress in human lung and pancreatic xenografts.

Clinical Trial

Molecular Weight

296.44

Formula

C₁₈H₃₂O₃

CAS No.

57818-44-7

Appearance

Liquid

Color

Colorless to light yellow

SMILES

CCCCC/C=C\C/C=C\CCCCCCC(O)C(O)=O

Structure Classification

Ketones, Aldehydes, Acids

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Pure form	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 250 mg/mL (843.34 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.3734 mL	16.8668 mL	33.7336 mL
5 mM	0.6747 mL	3.3734 mL	6.7467 mL
10 mM	0.3373 mL	1.6867 mL	3.3734 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (7.02 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (7.02 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (7.02 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.06%

Select Batch:

Data Sheet (267 KB) SDS (252 KB)

COA (238 KB) HNMR (191 KB) LCMS (115 KB)

Handling Instructions (2659 KB)

References

[1]. Muñoz-Guardiola P, et al. The anti-cancer drug ABTL0812 induces ER stress-mediated cytotoxic autophagy by increasing dihydroceramide levels in cancer cells. Autophagy. 2020 May 13. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.3734 mL	16.8668 mL	33.7336 mL	84.3341 mL
	5 mM	0.6747 mL	3.3734 mL	6.7467 mL	16.8668 mL
	10 mM	0.3373 mL	1.6867 mL	3.3734 mL	8.4334 mL
	15 mM	0.2249 mL	1.1245 mL	2.2489 mL	5.6223 mL
	20 mM	0.1687 mL	0.8433 mL	1.6867 mL	4.2167 mL
	25 mM	0.1349 mL	0.6747 mL	1.3493 mL	3.3734 mL
	30 mM	0.1124 mL	0.5622 mL	1.1245 mL	2.8111 mL
	40 mM	0.0843 mL	0.4217 mL	0.8433 mL	2.1084 mL
	50 mM	0.0675 mL	0.3373 mL	0.6747 mL	1.6867 mL
	60 mM	0.0562 mL	0.2811 mL	0.5622 mL	1.4056 mL
	80 mM	0.0422 mL	0.2108 mL	0.4217 mL	1.0542 mL
	100 mM	0.0337 mL	0.1687 mL	0.3373 mL	0.8433 mL

ABTL-0812 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

ABTL-081257818-44-7α-Hydroxylinoleic acidABTL0812ABTL 0812AutophagyInhibitorinhibitorinhibit

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