Artemisinin (Synonyms: Qinghaosu; NSC 369397)

Name: Artemisinin
Brand: MedChemExpress
SKU: HY-B0094
Rating: 5.0 (12 reviews)

Cat. No.: HY-B0094 Purity: 98.40%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Artemisinin (Qinghaosu), a sesquiterpene lactone, is an anti-malarial agent isolated from the aerial parts of Artemisia annua L. plants. Artemisinin inhibits AKT signaling pathway by decreasing pAKT in a dose-dependent manner. Artemisinin reduces cancer cell proliferation, migration, invasion, tumorigenesis and metastasis and has neuroprotective effects.

For research use only. We do not sell to patients.

Artemisinin Chemical Structure

CAS No. : 63968-64-9

Size	Price	Stock	Quantity

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Solid + Solvent
10 mM * 1 mL in DMSO ready for reconstitution	USD 77	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 77	In-stock	Estimated Time of Arrival: December 31
Solid
200 mg	USD 70	In-stock	Estimated Time of Arrival: December 31
500 mg	USD 100	In-stock	Estimated Time of Arrival: December 31
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Customer Review

Based on 12 publication(s) in Google Scholar

Other Forms of Artemisinin:

Artemisinin-d₃ Get quote

: Artemisinin purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2019 Oct;118:109383. [Abstract]; ART (Artemisinin) suppresses the phosphorylation of AKT in UMRC-2 xenograft tumor. Tumors are homogenized in RIPA buffer. The protein levels were measured by western blot analysis.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Artemisinin (Qinghaosu), a sesquiterpene lactone, is an anti-malarial agent isolated from the aerial parts of Artemisia annua L. plants^[1]. Artemisinin inhibits AKT signaling pathway by decreasing pAKT in a dose-dependent manner. Artemisinin reduces cancer cell proliferation, migration, invasion, tumorigenesis and metastasis and has neuroprotective effects^[2].

IC₅₀ & Target^[2]

Plasmodium

In Vitro

Artemisinin (Qinghaosu) (25 or 50 μM; 24 hours) concentration-dependently suppresses Aβ25-35 induced cytotoxicity in PC12 cells^[1].
Artemisinin (1-100 μM; 24 hours) selectively inhibits cancer cell growth in a dose-dependent manner with IC₅₀ values of 31.30 ± 0.73 μM in UMRC-2 cells and 23.97 ± 0.92 CAKI-2 cells^[2].
Artemisinin (25, 50 μM; 24 hours) suppresses the phosphorylation of AKT in UMRC-2 and CAKI-2 cells in a dose-dependent manner^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cytotoxicity Assay^[1]

Cell Line:	PC12 cells
Concentration:	25 or 50 μM
Incubation Time:	24 hours
Result:	Protected and rescue PC12 cells against Aβ25-35-induced cell death.

Cell Viability Assay^[2]

Cell Line:	RCC cells, RCC cell lines UMRC-2 and CAKI-2, and normal renal cell HK-2
Concentration:	1, 5, 10, 50, and 100 μM
Incubation Time:	24 hours
Result:	Selectively inhibited cancer cell growth in a dose-dependent manner.

Western Blot Analysis^[2]

Cell Line:	UMRC-2 and CAKI-2 cells
Concentration:	25, 50 μM
Incubation Time:	24 hours
Result:	Decreased pAKT in a dose-dependent manner.

In Vivo

Artemisinin (gavage; 20 mg/kg/day; for two weeks) suppresses UMRC-2 xenograft tumor growth^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	4-6 weeks old male nude mice^[2]
Dosage:	20 mg/kg
Administration:	gavage; every day for two weeks
Result:	Suppressed UMRC-2 xenograft tumor growth.

Clinical Trial

Molecular Weight

282.33

Formula

C₁₅H₂₂O₅

CAS No.

63968-64-9

Appearance

Solid

Color

White to off-white

SMILES

O=C1[C@H](C)[C@]2([H])CC[C@@H](C)[C@]3([H])CC[C@@](O4)(C)OO[C@]32[C@]4([H])O1

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	1 year
	-20°C	6 months

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (177.10 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.5420 mL	17.7098 mL	35.4195 mL
5 mM	0.7084 mL	3.5420 mL	7.0839 mL
10 mM	0.3542 mL	1.7710 mL	3.5420 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (7.37 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (7.37 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (7.37 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.40%

Select Batch:

Data Sheet (286 KB) SDS (0 KB)

COA (261 KB) HNMR (276 KB) RP-HPLC (212 KB) MS (177 KB)

Handling Instructions (2659 KB)

References

[1]. Zeng Z, et al. Artemisinin protects PC12 cells against β-amyloid-induced apoptosis through activation of the ERK1/2 signaling pathway. Redox Biol. 2017 Apr 4;12:625-633. [Content Brief]

[2]. Lin SP, et al. Artemisinin Prevents Glutamate-Induced Neuronal Cell Death Via Akt Pathway Activation. Front Cell Neurosci. 2018 Apr 20;12:108. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.5420 mL	17.7098 mL	35.4195 mL	88.5489 mL
	5 mM	0.7084 mL	3.5420 mL	7.0839 mL	17.7098 mL
	10 mM	0.3542 mL	1.7710 mL	3.5420 mL	8.8549 mL
	15 mM	0.2361 mL	1.1807 mL	2.3613 mL	5.9033 mL
	20 mM	0.1771 mL	0.8855 mL	1.7710 mL	4.4274 mL
	25 mM	0.1417 mL	0.7084 mL	1.4168 mL	3.5420 mL
	30 mM	0.1181 mL	0.5903 mL	1.1807 mL	2.9516 mL
	40 mM	0.0885 mL	0.4427 mL	0.8855 mL	2.2137 mL
	50 mM	0.0708 mL	0.3542 mL	0.7084 mL	1.7710 mL
	60 mM	0.0590 mL	0.2952 mL	0.5903 mL	1.4758 mL
	80 mM	0.0443 mL	0.2214 mL	0.4427 mL	1.1069 mL
	100 mM	0.0354 mL	0.1771 mL	0.3542 mL	0.8855 mL

Artemisinin Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Artemisinin63968-64-9Qinghaosu NSC 369397NSC369397NSC 369397NSC-369397HCVParasiteAktFerroptosisHepatitis C virusPKBProtein kinase BInhibitorinhibitorinhibit

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Artemisinin (Synonyms: Qinghaosu; NSC 369397)

Customer Review

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Artemisinin Related Antibodies

11 Publications Citing Use of MCE Artemisinin

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Artemisinin Related Antibodies

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Complete Stock Solution Preparation Table

Artemisinin Related Classifications

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