DPH

Name: DPH
Brand: MedChemExpress
SKU: HY-12070
Rating: 5.0 (6 reviews)

Cat. No.: HY-12070 Purity: 99.20%: FAQs
Data Sheet SDS COA Handling Instructions

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DPH is a potent cell permeable c-Abl activator, which displays potent enzymatic and cellular activity in stimulating c-Abl activation.

For research use only. We do not sell to patients.

DPH Chemical Structure

CAS No. : 484049-04-9

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent
10 mM * 1 mL in DMSO ready for reconstitution	USD 161	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 161	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 94	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 146	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 588	In-stock	Estimated Time of Arrival: December 31
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Based on 6 publication(s) in Google Scholar

4 Publications Citing Use of MCE DPH

: DPH purchased from MedChemExpress. Usage Cited in: Toxicol Sci. 2018 Sep 1;165(1):232-243. [Abstract]; Treatment of SN4741 cells with DPH, a c-Abl activator, results in a significant phosphorylation of GSK3β-Y216

: DPH purchased from MedChemExpress. Usage Cited in: Mol Cell Neurosci. 2017 Dec;85:226-234. [Abstract]; In a separate experiment, mice are injected with DPH and euthanized 4 hours later. DPH increases hippocampal p190RhoGAP phosphorylation. Representative blots below.

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Description

DPH is a potent cell permeable c-Abl activator, which displays potent enzymatic and cellular activity in stimulating c-Abl activation.

In Vitro

DPH binds to the myristoyl binding site and prevents the formation of the bent conformation of the αI helix through steric hindrance, a mode of action distinct from the previously identified allosteric c-Abl inhibitor, GNF-2, that also binds to the myristoyl binding site. DPH represents the first cell-permeable, small-molecule tool compound for c-Abl activation^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

336.32

Formula

C₁₈H₁₃FN₄O₂

CAS No.

484049-04-9

Appearance

Solid

Color

White to off-white

SMILES

O=C1NC(C(N1)C2=CN(N=C2C3=CC=C(C=C3)F)C4=CC=CC=C4)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (148.67 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.9734 mL	14.8668 mL	29.7336 mL
5 mM	0.5947 mL	2.9734 mL	5.9467 mL
10 mM	0.2973 mL	1.4867 mL	2.9734 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.43 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (7.43 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (7.43 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.20%

Select Batch:

Data Sheet (278 KB) SDS (0 KB)

COA (255 KB) LCMS (264 KB)

Handling Instructions (2659 KB)

References

[1]. Yang J, et al. Discovery and characterization of a cell-permeable, small-molecule c-Abl kinase activator that binds to the myristoyl binding site. Chem Biol. 2011 Feb 25;18(2):177-86 [Content Brief]

[2]. Shapiro LP, et al. Corticosteroid-induced dendrite loss and behavioral deficiencies can be blocked by activation of Abl2/Arg kinase. Mol Cell Neurosci. 2017 Oct 26;85:226-234. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.9734 mL	14.8668 mL	29.7336 mL	74.3340 mL
	5 mM	0.5947 mL	2.9734 mL	5.9467 mL	14.8668 mL
	10 mM	0.2973 mL	1.4867 mL	2.9734 mL	7.4334 mL
	15 mM	0.1982 mL	0.9911 mL	1.9822 mL	4.9556 mL
	20 mM	0.1487 mL	0.7433 mL	1.4867 mL	3.7167 mL
	25 mM	0.1189 mL	0.5947 mL	1.1893 mL	2.9734 mL
	30 mM	0.0991 mL	0.4956 mL	0.9911 mL	2.4778 mL
	40 mM	0.0743 mL	0.3717 mL	0.7433 mL	1.8583 mL
	50 mM	0.0595 mL	0.2973 mL	0.5947 mL	1.4867 mL
	60 mM	0.0496 mL	0.2478 mL	0.4956 mL	1.2389 mL
	80 mM	0.0372 mL	0.1858 mL	0.3717 mL	0.9292 mL
	100 mM	0.0297 mL	0.1487 mL	0.2973 mL	0.7433 mL

DPH Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

DPH484049-04-9Bcr-AblInhibitorinhibitorinhibit

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DPH

Customer Review

DPH Related Antibodies

4 Publications Citing Use of MCE DPH

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•Related Screening Libraries:

•Related Small Molecules:

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Biological Activity

Purity & Documentation

References

Customer Review

DPH Related Antibodies

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Complete Stock Solution Preparation Table

DPH Related Classifications

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