2. Vitamin D Related/Nuclear Receptor
  3. Androgen Receptor
  4. Flutamide

Flutamide  (Synonyms: SCH 13521)

Cat. No.: HY-B0022 Purity: 99.90%
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Flutamide is an Androgen Receptor antagonist with Ki=55 nM. Flutamide inhibits prostate cancer progression and has synergistic effects with Docetaxel (HY-B0011). Flutamide also has the potential to protect against hyperthermia-induced multiple organ dysfunction syndrome.

For research use only. We do not sell to patients.

Flutamide Chemical Structure

Flutamide Chemical Structure

CAS No. : 13311-84-7

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10 mM * 1 mL in DMSO
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Based on 5 publication(s) in Google Scholar

Other Forms of Flutamide:

Flutamide Related Antibodies

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  • Purity & Documentation

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Description

Flutamide is an Androgen Receptor antagonist with Ki=55 nM. Flutamide inhibits prostate cancer progression and has synergistic effects with Docetaxel (HY-B0011). Flutamide also has the potential to protect against hyperthermia-induced multiple organ dysfunction syndrome[1][2][3][4][5][6][7].

In Vitro

The active metabolite of Flutamide, is Flutamide-OH. Both of them directly bind rat anterior pituitary androgen receptor (Ki=55 nM)[1].
Flutamide does not affect the proliferation of an androgen-sensitive clone of the mouse mammary carcinoma Shionogi SC-l 15 cells in culture, shows only antiandrogenic effect, but not androgenic effect[2].
Flutamide provides treatment for prostate cancer when used along with Leuprolide[3].
Flutamide has cytotoxic activity against PC3 and LNCap (IC50s 20 μM and 12 μM, respectively). Flutamide (10 μM, 5 μM; 48 h) induces apoptosis and reduces cell migration and colonization in PC3 and LNCap cells[4].
Flutamide also downregulates the expression of KLK2 and EMT pathway genes in cells[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Flutamide Related Antibodies
In Vivo

Flutamide causes a markedly reduction in rat ventral prostate weight from 319 mg to 245 mg. A combination of Flutamide and LHRH agonist, induces an additive effect with a decrease in prostate weight to 101 mg, and an marked drop in prostatic ODC activity[5].
Flutamide (12.5-50 mg/kg; sc; once daily for 3 days) alleviates heat stroke in heat-stressed mice[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Clinical Trial
Molecular Weight

276.21

Formula

C11H11F3N2O3
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

CC(C)C(NC1=CC=C([N+]([O-])=O)C(C(F)(F)F)=C1)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (362.04 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.6204 mL 18.1022 mL 36.2043 mL
5 mM 0.7241 mL 3.6204 mL 7.2409 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (9.05 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (9.05 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.90%

COA (247 KB) LCMS (306 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.6204 mL 18.1022 mL 36.2043 mL 90.5108 mL
5 mM 0.7241 mL 3.6204 mL 7.2409 mL 18.1022 mL
10 mM 0.3620 mL 1.8102 mL 3.6204 mL 9.0511 mL
15 mM 0.2414 mL 1.2068 mL 2.4136 mL 6.0341 mL
20 mM 0.1810 mL 0.9051 mL 1.8102 mL 4.5255 mL
25 mM 0.1448 mL 0.7241 mL 1.4482 mL 3.6204 mL
30 mM 0.1207 mL 0.6034 mL 1.2068 mL 3.0170 mL
40 mM 0.0905 mL 0.4526 mL 0.9051 mL 2.2628 mL
50 mM 0.0724 mL 0.3620 mL 0.7241 mL 1.8102 mL
60 mM 0.0603 mL 0.3017 mL 0.6034 mL 1.5085 mL
80 mM 0.0453 mL 0.2263 mL 0.4526 mL 1.1314 mL
100 mM 0.0362 mL 0.1810 mL 0.3620 mL 0.9051 mL
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Flutamide Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Flutamide13311-84-7SCH 13521SCH13521SCH-13521Androgen ReceptorInhibitorinhibitorinhibit

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