1. GPCR/G Protein Neuronal Signaling
  2. Adrenergic Receptor
  3. Nebivolol hydrochloride

Nebivolol hydrochloride  (Synonyms: R 065824 hydrochloride)

Cat. No.: HY-B0203A Purity: 99.85%
COA Handling Instructions

Nebivolol (R 065824) hydrochloride is an orally active beta receptor blocker and has the high beta(1)-receptor affinity.Nebivolol hydrochloride has direct vasodilator properties and adrenergic blocking characteristics. Nebivolol hydrochloride can be used for the research of kinds of diseases such as hypertension, coronary artery disease, congestive heart failure and ischemic heart disease.

For research use only. We do not sell to patients.

Nebivolol hydrochloride Chemical Structure

Nebivolol hydrochloride Chemical Structure

CAS No. : 152520-56-4

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Solid + Solvent
10 mM * 1 mL in DMSO
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10 mM * 1 mL in DMSO USD 33 In-stock
Solid
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10 mg USD 45 In-stock
50 mg USD 100 In-stock
100 mg USD 160 In-stock
500 mg USD 480 In-stock
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Customer Review

Based on 2 publication(s) in Google Scholar

Other Forms of Nebivolol hydrochloride:

Top Publications Citing Use of Products

2 Publications Citing Use of MCE Nebivolol hydrochloride

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  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Nebivolol (R 065824) hydrochloride is an orally active beta receptor blocker and has the high beta(1)-receptor affinity.Nebivolol hydrochloride has direct vasodilator properties and adrenergic blocking characteristics. Nebivolol hydrochloride can be used for the research of kinds of diseases such as hypertension, coronary artery disease, congestive heart failure and ischemic heart disease[1][2].

In Vitro

Nebivolol hydrochloride improves endothelial dysfunction via its strong stimulatory effects on the activity of the endothelial nitric oxide synthase and via its antioxidative properties[1].
? Nebivolol hydrochloride (0.1 μM-10 μM, 1, 2, 4, 7 or 14 days) inhibits haCSMC or haEC proliferation with IC50 values of ~ 6.0 μM[2].
? Nebivolol hydrochloride (0.1 μM-10 μM, 24 h) induces a moderate rate of apoptosis[2].
? ? Nebivolol hydrochloride (0.1, 1, 5,10 μM, 4 days)increases NO formation? and decreases endothelin-1 secretion in HaCEs[2]

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay[2]

Cell Line: haCSMCs (human coronary smooth muscle cells) and haECs (endothelial cells)
Concentration: 0.1 μM-10 μM
Incubation Time: 1, 2, 4, 7 or 14 days
Result: Reduced proliferation in HaECs and HaCSMCs in a concentration- and time-dependent.

Apoptosis Analysis[2]

Cell Line: haCSMCs and haECs
Concentration: 0.1 μM-10 μM
Incubation Time: 24 h
Result: Induced a moderate apoptosis in concentration-dependent and showed a decrease of haCSMCs in the S-phase by 66%.
Clinical Trial
Molecular Weight

441.90

Formula

C22H26ClF2NO4

CAS No.
Appearance

Solid

Color

White to off-white

SMILES

FC1=CC=C2C(CC[C@]([C@@H](O)CNC[C@H](O)[C@@]3([H])CCC(C=C(F)C=C4)=C4O3)([H])O2)=C1.Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (226.30 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 1.79 mg/mL (4.05 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.2630 mL 11.3148 mL 22.6296 mL
5 mM 0.4526 mL 2.2630 mL 4.5259 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (4.71 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.08 mg/mL (4.71 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.85%

References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 2.2630 mL 11.3148 mL 22.6296 mL 56.5739 mL
DMSO 5 mM 0.4526 mL 2.2630 mL 4.5259 mL 11.3148 mL
10 mM 0.2263 mL 1.1315 mL 2.2630 mL 5.6574 mL
15 mM 0.1509 mL 0.7543 mL 1.5086 mL 3.7716 mL
20 mM 0.1131 mL 0.5657 mL 1.1315 mL 2.8287 mL
25 mM 0.0905 mL 0.4526 mL 0.9052 mL 2.2630 mL
30 mM 0.0754 mL 0.3772 mL 0.7543 mL 1.8858 mL
40 mM 0.0566 mL 0.2829 mL 0.5657 mL 1.4143 mL
50 mM 0.0453 mL 0.2263 mL 0.4526 mL 1.1315 mL
60 mM 0.0377 mL 0.1886 mL 0.3772 mL 0.9429 mL
80 mM 0.0283 mL 0.1414 mL 0.2829 mL 0.7072 mL
100 mM 0.0226 mL 0.1131 mL 0.2263 mL 0.5657 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Nebivolol hydrochloride Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Nebivolol hydrochloride
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