Spectinomycin dihydrochloride

Name: Spectinomycin dihydrochloride
Brand: MedChemExpress
SKU: HY-B0438
Rating: 4.5 (3 reviews)

Cat. No.: HY-B0438 Purity: 99.61%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Spectinomycin dihydrochloride is a broad-spectrum antibiotic and inhibits the growth of a variety of gram-positive and gram-negative organisms. Spectinomycin dihydrochloride acts by selectively targeting to the bacterial ribosome and interrupting protein synthesis. Spectinomycin dihydrochloride is also a noncompetitive inhibitor of td intron RNA with an K_i value of 7.2 mM^-.

For research use only. We do not sell to patients.

Spectinomycin dihydrochloride Chemical Structure

CAS No. : 21736-83-4

Size	Price	Stock	Quantity
Solid + Solvent
10 mM * 1 mL in Water ready for reconstitution	USD 61	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in Water	USD 61	In-stock	Estimated Time of Arrival: December 31
Solid
500 mg	USD 55	In-stock	Estimated Time of Arrival: December 31
1 g	USD 66	In-stock	Estimated Time of Arrival: December 31
5 g	USD 99	In-stock	Estimated Time of Arrival: December 31
25 g	USD 242	In-stock	Estimated Time of Arrival: December 31
50 g		Get quote

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Customer Review

Based on 3 publication(s) in Google Scholar

Other Forms of Spectinomycin dihydrochloride:

Spectinomycin dihydrochloride pentahydrate In-stock
Spectinomycin Get quote

3 Publications Citing Use of MCE Spectinomycin dihydrochloride

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

bacterial ribosomal subunit^[5]

In Vitro

Spectinomycin dihydrochloride selectively inhibits protein synthesis in cells and in extracts of Escherichia coli:
Spectinomycin dihydrochloride (50 μg/mL) inhibits Escherichia coli growth rapidly and reversibly, and suppresses amino acid incorporation immediately^[1].
Spectinomycin dihydrochloride (1 μg/mL or 3 μM) inhibits polypeptide synthesis directed either by endogenous messenger RNA or by MS-2 bacteriophage RNA, with maximum inhibition of 70-80% in extracts of Escherichia coli^[1]^[1].
Spectinomycin dihydrochloride blocks the translocation of peptidyl-tRNAs from A-site to P-site by inhibiting the binding of elongation factor G to the ribosome^[2].
Spectinomycin dihydrochloride interacts specifically with the residues G1064 and 01192 in 16S rRNA and potentially makes it inactive^[2].
Spectinomycin dihydrochloride exhibits splicing inhibition and dependent on pH changes and Mg²⁺ concentration, indicating electrostatic interactions with the intron RNA^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Spectinomycin dihydrochloride Related Antibodies

In Vivo

Spectinomycin dihydrochloride (20 mg/kg; i.m.; 20-100 mg/kg; 9 d) shows the safety in healthy chicks^[4].
Spectinomycin dihydrochloride (10 mg/kg; i.v.; single dose) has the major elimination pathway by renal excretion, approximately 55% is excreted into the urine in unchanged form^[5].
Pharmacokinetics of Spectinomycin dihydrochloride in Rat^[5]

Parameter	C₀ (μg/mL)	AUC_0-∞ (μg•h/mL)	V_d (L/kg)	CL (L/h/kg)	MRT (h)	T_1/2α (h)	T_1/2β (h)	T_1/2γ (h)	f_e	CL_renal (L/h/kg)	E_ratio
Non atrioventricular analysis	44.3	16.8	0.756	0.602	0.757	/	/	/	0.553	0.359	1.00
Three-compartment model	37.8	15.7	0.747	0.649	1.11	/	0.237	0.754	19.5	/	/

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Arbor Acres plus broiler chicks (15-day-old)^[4]
Dosage:	20 mg/kg, 60 mg/kg, 100 mg/kg
Administration:	Intramuscular injection (chest muscles); 9 days
Result:	Showed biosecurity of 20 mg/kg by complete blood count, biochemical parameters, histopathological, clinical signs, body weight gain, and feed conversion ratio (FCR). Resulted minor toxicity of 60 mg/kg.

Molecular Weight

405.27

Formula

C₁₄H₂₆Cl₂N₂O₇

CAS No.

21736-83-4

Appearance

Solid

Color

White to off-white

SMILES

CN[C@@H]([C@H]([C@@H](NC)[C@@H]1O)O)[C@]2([H])[C@]1([H])O[C@@](O[C@H](C)C3)([H])[C@](O)(C3=O)O2.Cl.Cl

Structure Classification

Initial Source

Microorganisms

bacterium Streptomyces spectabilis

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

H₂O : ≥ 100 mg/mL (246.75 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.4675 mL	12.3375 mL	24.6749 mL
5 mM	0.4935 mL	2.4675 mL	4.9350 mL
10 mM	0.2467 mL	1.2337 mL	2.4675 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 50 mg/mL (123.37 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.61%

Select Batch:

Data Sheet (297 KB) SDS (393 KB)

COA (257 KB) HNMR (255 KB) RP-HPLC (155 KB)

Handling Instructions (2659 KB)

References

[1]. Davies J, et al. Inhibition of protein synthesis by spectinomycin. Science. 1965 Sep 3;149(3688):1096-8. [Content Brief]

[2]. Brink MF, et al. Spectinomycin interacts specifically with the residues G1064 and C1192 in 16S rRNA, thereby potentially freezing this molecule into an inactive conformation. Nucleic Acids Res. 1994 Feb 11;22(3):325-31. [Content Brief]

[3]. Park IK, et al. Spectinomycin inhibits the self-splicing of the group 1 intron RNA. Biochem Biophys Res Commun. 2000 Mar 16;269(2):574-9. [Content Brief]

[4]. Khan EA, et al. Safety evaluation study of lincomycin and spectinomycin hydrochloride intramuscular injection in chickens. Toxicol Rep. 2022 Jan 29;9:204-209. [Content Brief]

[5]. Madhura DB, et al. Pharmacokinetic profile of spectinomycin in rats. Pharmazie. 2013 Aug;68(8):675-6. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	2.4675 mL	12.3375 mL	24.6749 mL	61.6873 mL
	5 mM	0.4935 mL	2.4675 mL	4.9350 mL	12.3375 mL
	10 mM	0.2467 mL	1.2337 mL	2.4675 mL	6.1687 mL
	15 mM	0.1645 mL	0.8225 mL	1.6450 mL	4.1125 mL
	20 mM	0.1234 mL	0.6169 mL	1.2337 mL	3.0844 mL
	25 mM	0.0987 mL	0.4935 mL	0.9870 mL	2.4675 mL
	30 mM	0.0822 mL	0.4112 mL	0.8225 mL	2.0562 mL
	40 mM	0.0617 mL	0.3084 mL	0.6169 mL	1.5422 mL
	50 mM	0.0493 mL	0.2467 mL	0.4935 mL	1.2337 mL
	60 mM	0.0411 mL	0.2056 mL	0.4112 mL	1.0281 mL
	80 mM	0.0308 mL	0.1542 mL	0.3084 mL	0.7711 mL
	100 mM	0.0247 mL	0.1234 mL	0.2467 mL	0.6169 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.