1. Cell Cycle/DNA Damage
    NF-κB
    Cell Cycle/DNA Damage
  2. PPAR
    RAD51

T0070907 (Synonyms: T-0070907; T 0070907)

Cat. No.: HY-13202 Purity: 99.98%
Data Sheet SDS Handling Instructions

T0070907 is a potent PPARγ antagonist and a potential RAD51 inhibitor, with the IC50 value of 1 nM towards PPARγ.

For research use only. We do not sell to patients.
T0070907 Chemical Structure

T0070907 Chemical Structure

CAS No. : 313516-66-4

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10 mM * 1 mL in DMSO $55 In-stock
5 mg $50 In-stock
10 mg $60 In-stock
50 mg $216 In-stock
100 mg $412 In-stock
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Description

T0070907 is a potent PPARγ antagonist and a potential RAD51 inhibitor, with the IC50 value of 1 nM towards PPARγ.

IC50 & Target

IC50: 1 nM (PPARγ)

In Vitro

T0070907 (50 μM) pre-treatment impairs repair of IR-induced DNA DSBs in both ME-180 and SiHa cells treated with irradiated (4 Gy). T0070907 (0-50 μM) significantly decreases the levels of DNA-PKcs and RAD51 proteins in ME-180 and SiHa cells[1]. T0070907 (50 μM) treatment reduces the levels of α- and β-tubulin protein in a time-dependent manner, decreases the synthesis of DNA, and prevents the radiation-induced alterations in the cell cycle regulatory proteins of ME180 and SiHa cells[2]. T0070907 (10 µM) has cytotoxicity in an adipocyte-specific and PPARγ-independent manner. T0070907 increases oxidative stress in immature adipocytes[3]. T0070907 (1 μM) blocks the induction of adipogenesis by various treatments of the adipogenic cell line 3T3-L1. T0070907 covalently modifies PPAR on cysteine 313 in helix 3 of human PPAR 2[4].

References
Preparing Stock Solutions
Concentration Volume (DMSO) Mass 1 mg 5 mg 10 mg
1 mM 3.6015 mL 18.0076 mL 36.0153 mL
5 mM 0.7203 mL 3.6015 mL 7.2031 mL
10 mM 0.3602 mL 1.8008 mL 3.6015 mL
Kinase Assay
[4]

To determine the binding affinity of T0070907 to the PPARs, scintillation proximity assay (SPA) is performed with the following modifications. A 90 μL reaction contains SPA buffer (10 mM K2HPO4, 10 mM KH2PO4, 2 mM EDTA, 50 mM NaCl, 1 mM dithiothreitol, 2 mM CHAPS, 10% (v/v) glycerol, pH 7.1), 50 ng of GST-PPAR (or 150 ng of GST-PPAR), 5 nM 3H-labeled radioligands, and 5 μL of T0070907 in Me2SO. After incubation for 1 h at room temperature, 10 μL of polylysine-coated SPA beads (at 20 mg/mL in SPA buffer) are added, and the mixture is incubated for 1 h before reading in Packard Topcount. [3H]Rosiglitazone is used for PPAR, and [3H]GW2433 is used for PPAR and PPAR.

References
M.Wt

277.66

Formula

C₁₂H₈ClN₃O₃

CAS No.

313516-66-4

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Shipping

Room temperature in continental US; may vary elsewhere

Solvent & Solubility

10 mM in DMSO

* "<1 mg/mL" means slightly soluble or insoluble. "≥" means soluble, but saturation unknown.

Purity: 99.98%

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Product Name:
T0070907
Cat. No.:
HY-13202
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