1. Cell Cycle/DNA Damage
    NF-κB
  2. PPAR
    RAD51

T0070907 

Cat. No.: HY-13202 Purity: 99.98%
Data Sheet SDS Handling Instructions

T0070907 is a potent PPARγ antagonist and a potential RAD51 inhibitor, with the IC50 value of 1 nM towards PPARγ.

For research use only. We do not sell to patients.
T0070907 Chemical Structure

T0070907 Chemical Structure

CAS No. : 313516-66-4

Size Price Stock Quantity
Free Sample (0.5-1 mg)   Apply now  
10 mM * 1 mL in DMSO $55 In-stock
5 mg $50 In-stock
10 mg $60 In-stock
50 mg $216 In-stock
100 mg $412 In-stock
200 mg   Get quote  
500 mg   Get quote  

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    T0070907 purchased from MCE. Usage Cited in: J Mol Neurosci. 2015 Aug;56(4):848-57.

    Effect of LXA4 on astrocyte viability. Cell viability is measured by MTT reduction assay. Results are expressed as the percentage of MTT reduction normalized to the control group (100 %). The MTT reduction is reduced by OGDR, but is significantly increased by LXA4. The effect is not antagonized by Boc2, but partly reversed by U73122 and T0070907.

    T0070907 purchased from MCE. Usage Cited in: J Mol Neurosci. 2015 Aug;56(4):848-57.

    a Statistical analysis showing the prevention effect of minocycline pretreatment (40 mg/kg/day) on CUS-, CRS- or CSDS-induced decreases in hippocampal microglial numbers. b, c Statistical analysis showing the prevention effect of minocycline pretreatment (40 mg/kg/day) on CUS-, CRS- or CSDS-induced increases in the immobile time in the TST (b) and FST (c). d, e Statistical analysis showing the prevention effect of minocycline pretreatment (40 mg/kg/day) on CUS-, CRS- or CSDS-induced decreases in
    • Biological Activity

    • Protocol

    • Technical Information

    • Purity & Documentation

    • References

    Description

    T0070907 is a potent PPARγ antagonist and a potential RAD51 inhibitor, with the IC50 value of 1 nM towards PPARγ.

    IC50 & Target

    IC50: 1 nM (PPARγ)

    In Vitro

    T0070907 (50 μM) pre-treatment impairs repair of IR-induced DNA DSBs in both ME-180 and SiHa cells treated with irradiated (4 Gy). T0070907 (0-50 μM) significantly decreases the levels of DNA-PKcs and RAD51 proteins in ME-180 and SiHa cells[1]. T0070907 (50 μM) treatment reduces the levels of α- and β-tubulin protein in a time-dependent manner, decreases the synthesis of DNA, and prevents the radiation-induced alterations in the cell cycle regulatory proteins of ME180 and SiHa cells[2]. T0070907 (10 µM) has cytotoxicity in an adipocyte-specific and PPARγ-independent manner. T0070907 increases oxidative stress in immature adipocytes[3]. T0070907 (1 μM) blocks the induction of adipogenesis by various treatments of the adipogenic cell line 3T3-L1. T0070907 covalently modifies PPAR on cysteine 313 in helix 3 of human PPAR 2[4].

    References
    Preparing Stock Solutions
    Concentration Volume Mass 1 mg 5 mg 10 mg
    1 mM 3.6015 mL 18.0076 mL 36.0153 mL
    5 mM 0.7203 mL 3.6015 mL 7.2031 mL
    10 mM 0.3602 mL 1.8008 mL 3.6015 mL
    Please refer to the solubility information to select the appropriate solvent.
    Kinase Assay
    [4]

    To determine the binding affinity of T0070907 to the PPARs, scintillation proximity assay (SPA) is performed with the following modifications. A 90 μL reaction contains SPA buffer (10 mM K2HPO4, 10 mM KH2PO4, 2 mM EDTA, 50 mM NaCl, 1 mM dithiothreitol, 2 mM CHAPS, 10% (v/v) glycerol, pH 7.1), 50 ng of GST-PPAR (or 150 ng of GST-PPAR), 5 nM 3H-labeled radioligands, and 5 μL of T0070907 in Me2SO. After incubation for 1 h at room temperature, 10 μL of polylysine-coated SPA beads (at 20 mg/mL in SPA buffer) are added, and the mixture is incubated for 1 h before reading in Packard Topcount. [3H]Rosiglitazone is used for PPAR, and [3H]GW2433 is used for PPAR and PPAR. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References
    Molecular Weight

    277.66

    Formula

    C₁₂H₈ClN₃O₃

    CAS No.

    313516-66-4

    Storage
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    Shipping

    Room temperature in continental US; may vary elsewhere

    Solvent & Solubility

    10 mM in DMSO

    * "<1 mg/mL" means slightly soluble or insoluble. "≥" means soluble, but saturation unknown.

    Purity: 99.98%

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    Product Name:
    T0070907
    Cat. No.:
    HY-13202
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