2. Anti-infection Apoptosis Epigenetics Cell Cycle/DNA Damage Immunology/Inflammation
  3. Parasite Caspase Bcl-2 Family PARP Pyroptosis
  4. Triclabendazole

Triclabendazole  (Synonyms: CGA89317)

Cat. No.: HY-B0621 Purity: 98.72%
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Triclabendazole is an orally active parasite inhibitor. Triclabendazole has anti-Leishmania activity and induces gasdermin E (GSDME)-dependent pyroptosis by caspase-3 activation. Triclabendazole can be used for the research of fasciola hepatica.

For research use only. We do not sell to patients.

Triclabendazole Chemical Structure

Triclabendazole Chemical Structure

CAS No. : 68786-66-3

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10 mM * 1 mL in DMSO
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Customer Review

Based on 4 publication(s) in Google Scholar

Other Forms of Triclabendazole:

Triclabendazole Related Antibodies

Top Publications Citing Use of Products

View All Parasite Isoform Specific Products:

View All Isoforms
Amebae Coccidia Leishmania Mite Plasmodium Toxoplasma Trypanosoma Schistosome

View All Caspase Isoform Specific Products:

View All Isoforms
Caspase 1 Caspase 2 Caspase 3 Caspase 4 Caspase 5 Caspase 6 Caspase 7 Caspase 8 Caspase 9 Caspase 10 Caspase 12 Caspase Caspase 11 Caspase-13

View All Bcl-2 Family Isoform Specific Products:

View All Isoforms
Bcl-2 Bcl-xL Bcl-W Mcl-1 Bfl-1 Bcl-B Bax Bak Bim

View All PARP Isoform Specific Products:

View All Isoforms
PARP PARP1 PARP2 PARP3 PARP4 TNKS1/PARP5A TNKS2/PARP5B PARP7 PARP10 PARP14 PARP15 PARP12 PARP16

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Triclabendazole is an orally active parasite inhibitor. Triclabendazole has anti-Leishmania activity and induces gasdermin E (GSDME)-dependent pyroptosis by caspase-3 activation. Triclabendazole can be used for the research of fasciola hepatica[1][2][3].

IC50 & Target

Caspase-8

 

Caspase-9

 

Caspase 3

 

Leishmania

 

Bcl-2

 

Bax

 

PARP

 

In Vitro

Triclabendazole (20-320 μM, 24-48 h) has cytotoxicity on MCF-7 and MDA-MB-231 cells[1].
Triclabendazole (40-160 μM, 24 h) induces apoptosis in MCF-7 and MDA-MB-231 cells[1].
Triclabendazole (0.97-500 μM, 48-72 h) have low cytotoxicity in Macrophages[3].
Triclabendazole (45.67 μM, 72 h) shows significant alterations in cell cycle with a decrease in G1 from 74.6% to 54.3% and an increase in S and G2 from 9.7% to 16.6% and 11.9% to 25.4%, respectively phases in promastigotes[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Triclabendazole Related Antibodies

Cell Cytotoxicity Assay[1]

Cell Line: MCF-7 and MDA-MB-231 cells
Concentration: 20 μM, 40 μM, 80 μM, 160 μM, 320 μM,
Incubation Time: 24 h, 48 h
Result: Significantly decreased the metabolic activity.

Apoptosis Analysis[1]

Cell Line: MCF-7 and MDA-MB-231 cells
Concentration: 40 μM, 80 μM, 160 μM
Incubation Time: 24 h
Result: Significantly induced apoptosis at 160 μM.
Up-regulated the expression of Bax and down-regulated the expression of Bcl-2.
Activated and cleaved caspase-8 and caspase-9 in a dose-dependent manner.
In Vivo

Triclabendazole (20-100 mg/kg, Intraperitoneal injection, twice a week for 2 weeks) has anti-tumor effect in the xenograft MDA-MB-231 cells model nude mice[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Clinical Trial
Molecular Weight

359.66

Formula

C14H9Cl3N2OS
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

CSC1=NC2=CC(OC3=CC=CC(Cl)=C3Cl)=C(Cl)C=C2N1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (278.04 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.7804 mL 13.9020 mL 27.8040 mL
5 mM 0.5561 mL 2.7804 mL 5.5608 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (6.95 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.08 mg/mL (5.78 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 98.72%

COA (247 KB) LCMS (256 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.7804 mL 13.9020 mL 27.8040 mL 69.5101 mL
5 mM 0.5561 mL 2.7804 mL 5.5608 mL 13.9020 mL
10 mM 0.2780 mL 1.3902 mL 2.7804 mL 6.9510 mL
15 mM 0.1854 mL 0.9268 mL 1.8536 mL 4.6340 mL
20 mM 0.1390 mL 0.6951 mL 1.3902 mL 3.4755 mL
25 mM 0.1112 mL 0.5561 mL 1.1122 mL 2.7804 mL
30 mM 0.0927 mL 0.4634 mL 0.9268 mL 2.3170 mL
40 mM 0.0695 mL 0.3476 mL 0.6951 mL 1.7378 mL
50 mM 0.0556 mL 0.2780 mL 0.5561 mL 1.3902 mL
60 mM 0.0463 mL 0.2317 mL 0.4634 mL 1.1585 mL
80 mM 0.0348 mL 0.1738 mL 0.3476 mL 0.8689 mL
100 mM 0.0278 mL 0.1390 mL 0.2780 mL 0.6951 mL
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Triclabendazole Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Triclabendazole68786-66-3CGA89317CGA 89317CGA-89317ParasiteCaspaseBcl-2 FamilyPARPPyroptosispoly ADP ribose polymeraseMCF-7MDA-MB-231THP-1LeishmaniasisInhibitorinhibitorinhibit

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