2-Methylcitric acid (Synonyms: Methylcitric acid)

Name: 2-Methylcitric acid
Brand: MedChemExpress
SKU: HY-113371
Rating: 4.5 (1 reviews)

Cat. No.: HY-113371 Purity: ≥97.0%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

2-Methylcitric acid (Methylcitric acid) is an endogenous metabolite in the 2-methylcitric acid cycle. 2-Methylcitric acid accumulates in methylmalonic and propionic acidemias and acts as a marker metabolite. 2-Methylcitric acid markedly inhibits ADP-stimulated and uncoupled respiration in mitochondria supported by glutamate.

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2-Methylcitric acid Chemical Structure

CAS No. : 6061-96-7

Size	Price	Stock	Quantity
5 mg	USD 140	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 240	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 480	In-stock	Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

Other Forms of 2-Methylcitric acid:

2-Methylcitric acid trisodium In-stock
2-Methylcitric acid-d₃ In-stock

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Biological Activity
Purity & Documentation
References
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Description

IC₅₀ & Target^[1]

Human Endogenous Metabolite

In Vitro

2-Methylcitric acid (Methylcitric acid) impairs glutamate metabolism and induces permeability transition in brain mitochondria. 2-Methylcitric acid inhibits glutamate dehydrogenase (GDH) activity at concentrations as low as 0.5 mM^[1].
2-Methylcitric acid (1-3 mM; 150 seconds) provokes a significant decrease in mitochondrial membrane potential and induced swelling in Ca²⁺-loaded mitochondria supported by different substrates^[1].
2-Methylcitric acid behaves as a potent inhibitor of glutamate oxidation by inhibiting glutamate dehydrogenase activity and as a permeability transition inducer, disturbing mitochondrial energy homeostasis. 2-Methylcitric acid does not significantly impair mitochondrial glutamate transport. 2-Methylcitric acid does not significantly impair glutamate mitochondrial transport in liver mitochondria^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

206.15

Formula

C₇H₁₀O₇

CAS No.

6061-96-7

Appearance

Solid

Color

White to off-white

SMILES

CC(C(O)=O)C(C(O)=O)(O)CC(O)=O

Structure Classification

Ketones, Aldehydes, Acids

Initial Source

Microorganisms

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (485.08 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 100 mg/mL (485.08 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.8508 mL	24.2542 mL	48.5084 mL
5 mM	0.9702 mL	4.8508 mL	9.7017 mL
10 mM	0.4851 mL	2.4254 mL	4.8508 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (12.13 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (12.13 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (12.13 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 100 mg/mL (485.08 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: ≥97.0%

Select Batch:

Data Sheet (273 KB) SDS (393 KB)

COA (248 KB) HNMR (245 KB) MS (68 KB)

Handling Instructions (2659 KB)

References

[1]. Palacios S, et al. Propionyl Coenzyme A Is a Common Intermediate in the 1,2-Propanediol and Propionate Catabolic Pathways Needed for Expression of the prpBCDE Operon during Growth of Salmonella enterica on 1,2-Propanediol. J Bacteriol. 2003 May;185(9):280 [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	4.8508 mL	24.2542 mL	48.5084 mL	121.2709 mL
	5 mM	0.9702 mL	4.8508 mL	9.7017 mL	24.2542 mL
	10 mM	0.4851 mL	2.4254 mL	4.8508 mL	12.1271 mL
	15 mM	0.3234 mL	1.6169 mL	3.2339 mL	8.0847 mL
	20 mM	0.2425 mL	1.2127 mL	2.4254 mL	6.0635 mL
	25 mM	0.1940 mL	0.9702 mL	1.9403 mL	4.8508 mL
	30 mM	0.1617 mL	0.8085 mL	1.6169 mL	4.0424 mL
	40 mM	0.1213 mL	0.6064 mL	1.2127 mL	3.0318 mL
	50 mM	0.0970 mL	0.4851 mL	0.9702 mL	2.4254 mL
	60 mM	0.0808 mL	0.4042 mL	0.8085 mL	2.0212 mL
	80 mM	0.0606 mL	0.3032 mL	0.6064 mL	1.5159 mL
	100 mM	0.0485 mL	0.2425 mL	0.4851 mL	1.2127 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

2-Methylcitric acid Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

2-Methylcitric acid6061-96-7Methylcitric acidEndogenous MetabolitemarkermetaboliteADP-stimulateduncoupledrespirationmitochondriaglutamateInhibitorinhibitorinhibit

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2-Methylcitric acid (Synonyms: Methylcitric acid)

Customer Review

Other Forms of 2-Methylcitric acid:

2-Methylcitric acid Related Antibodies

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•Related Small Molecules:

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Biological Activity

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References

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2-Methylcitric acid Related Antibodies

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Complete Stock Solution Preparation Table

2-Methylcitric acid Related Classifications

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