1. Click Chemistry
  2. Azide
  3. 4-Azido-L-phenylalanine

4-Azido-L-phenylalanine  (Synonyms: p-Azidophenylalanine; p-Azido-L-phenylalanine)

Cat. No.: HY-16714 Purity: 99.84%
COA Handling Instructions

4-Azido-L-phenylalanine is an unnatural amino acid, which is used as an effective vibrational reporter of local protein environments. 4-Azido-L-phenylalanine is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. Strain-promoted alkyne-azide cycloaddition (SPAAC) can also occur with molecules containing DBCO or BCN groups.

For research use only. We do not sell to patients.

4-Azido-L-phenylalanine Chemical Structure

4-Azido-L-phenylalanine Chemical Structure

CAS No. : 33173-53-4

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Customer Review

Based on 7 publication(s) in Google Scholar

Other Forms of 4-Azido-L-phenylalanine:

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Description

4-Azido-L-phenylalanine is an unnatural amino acid, which is used as an effective vibrational reporter of local protein environments. 4-Azido-L-phenylalanine is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. Strain-promoted alkyne-azide cycloaddition (SPAAC) can also occur with molecules containing DBCO or BCN groups.

In Vitro

In cell-free protein synthesis (CFPS) method, 0.9-1.7 mg/mL of modified soluble super-folder green fluorescent protein (sfGFP) containing either 4-Azido-L-phenylalanine or p-propargyloxy-l-phenylalanine (pPaF) accumulate in the CFPS solutions[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

206.20

Formula

C9H10N4O2

CAS No.
Appearance

Solid

Color

White to yellow

SMILES

O=C(O)[C@@H](N)CC1=CC=C(N=[N+]=[N-])C=C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

H2O : 50 mg/mL (242.48 mM; ultrasonic and adjust pH to 13 with NaOH)

H2O : 25 mg/mL (121.24 mM; ultrasonic and adjust pH to 11 with NaOH)

DMSO : 5 mg/mL (24.25 mM; ultrasonic and warming and adjust pH to 3 with HCl and heat to 80°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.8497 mL 24.2483 mL 48.4966 mL
5 mM 0.9699 mL 4.8497 mL 9.6993 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 99.84%

References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO / H2O / H2O 1 mM 4.8497 mL 24.2483 mL 48.4966 mL 121.2415 mL
5 mM 0.9699 mL 4.8497 mL 9.6993 mL 24.2483 mL
10 mM 0.4850 mL 2.4248 mL 4.8497 mL 12.1242 mL
15 mM 0.3233 mL 1.6166 mL 3.2331 mL 8.0828 mL
20 mM 0.2425 mL 1.2124 mL 2.4248 mL 6.0621 mL
H2O / H2O 25 mM 0.1940 mL 0.9699 mL 1.9399 mL 4.8497 mL
30 mM 0.1617 mL 0.8083 mL 1.6166 mL 4.0414 mL
40 mM 0.1212 mL 0.6062 mL 1.2124 mL 3.0310 mL
50 mM 0.0970 mL 0.4850 mL 0.9699 mL 2.4248 mL
60 mM 0.0808 mL 0.4041 mL 0.8083 mL 2.0207 mL
80 mM 0.0606 mL 0.3031 mL 0.6062 mL 1.5155 mL
100 mM 0.0485 mL 0.2425 mL 0.4850 mL 1.2124 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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4-Azido-L-phenylalanine
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