ABTS diammonium salt (Synonyms: AzBTS-(NH4)2)

Name: ABTS diammonium salt
Brand: MedChemExpress
SKU: HY-15902
Rating: 4.5 (2 reviews)

Cat. No.: HY-15902 Purity: 99.86%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

ABTS diammonium salt is a substrate for horseradish peroxidase (HRP) conjugate.

For research use only. We do not sell to patients.

ABTS diammonium salt Chemical Structure

CAS No. : 30931-67-0

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10 mM * 1 mL in DMSO ready for reconstitution	USD 55	In-stock	Estimated Time of Arrival: December 31
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10 mM * 1 mL in DMSO	USD 55	In-stock	Estimated Time of Arrival: December 31
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Based on 2 publication(s) in Google Scholar

2 Publications Citing Use of MCE ABTS diammonium salt

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Protocol
Purity & Documentation
References
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Description

ABTS diammonium salt is a substrate for horseradish peroxidase (HRP) conjugate.

In Vitro

A micro-technique of enzyme-linked immunosorbent assay (ELISA) using ABTS, as a substrate for HRP conjugate is studied. In a comparative study among 4 substrates, namely; 5-aminosalicylic acid (5AS), O-phenylenediamine (OPD), O-tolidine (OT) and ABTS, for HRP in terms of sensitivity, ABTS is the most sensitive, stable and the best in visuality by its bluish-green color^[1]. ABTS is a typical peroxidase substrate. For purification and characterization peroxidase positive transformants are cultivated in large scale (XL) under conditions that yield active protein in the culture supernatant. After 160 h cultivation an activity of 55,000 U/L in relation to the substrate ABTS is achieved and the supernatant containing the peroxidase is harvested. With ABTS as substrate the peroxidase activity falls significantly when the H₂O₂ concentration rose above 0.125 mM, indicating that the enzyme is inhibited by H₂O₂. Maximum reaction rates depending upon substrate tested reache values between 31.2 and 125 μM^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

548.68

Formula

C₁₈H₂₄N₆O₆S₄

CAS No.

30931-67-0

Appearance

Solid

Color

Light green to green

SMILES

CCN1C(C=CC(S(=O)(O)=O)=C2)=C2S/C1=N/N=C(SC3=C4C=CC(S(=O)(O)=O)=C3)\N4CC.N.N

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

H₂O : ≥ 50 mg/mL (91.13 mM)

DMSO : 20.83 mg/mL (37.96 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.8226 mL	9.1128 mL	18.2256 mL
5 mM	0.3645 mL	1.8226 mL	3.6451 mL
10 mM	0.1823 mL	0.9113 mL	1.8226 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture and light). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (3.79 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (3.79 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (3.79 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 50 mg/mL (91.13 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.86%

Select Batch:

Data Sheet (276 KB) SDS (0 KB)

COA (248 KB) HNMR (260 KB) LCMS (94 KB)

Handling Instructions (2659 KB)

References

[1]. Matsuda H, et al. Evaluation of ELISA with ABTS, 2-2'-azino-di-(3-ethylbenzthiazoline sulfonic acid), as the substrate of peroxidase and its application to the diagnosis of schistosomiasis. Jpn J Exp Med. 1984 Jun;54(3):131-8. [Content Brief]

[2]. Lauber C, et al. Identification, heterologous expression and characterization of a dye-decolorizing peroxidase of Pleurotus sapidus. AMB Express. 2017 Aug 23;7(1):164. [Content Brief]

Kinase Assay
^[2]

Enzyme activity is determined photometrically using a temperature controlled multi-mode plate reader or alternatively in a UV/Vis spectrophotometer. Reactions are initiated by addition of the enzyme. Enzyme activity is measured over a period of 10 min at 25°C at the appropriate wavelength for the substrate. One unit (1 U) is defined as the amount of enzyme that converts 1 µmol substrate per minute. Various H₂O₂ concentrations (0-1250 µM, enzyme concentrations (0.27-54 nM) and substrate concentrations are used to determine the enzyme activity. The activity of rPsaDyP vs ABTS is determined in 100 mM sodium acetate buffer at pH 3.8 and a final H₂O₂ concentration of 125 µM. Production of the ABTS cation radical is studied at 420 nm (ε₄₂₀ 36,000 L mol^-1 cm^-1)^[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

[1]. Matsuda H, et al. Evaluation of ELISA with ABTS, 2-2'-azino-di-(3-ethylbenzthiazoline sulfonic acid), as the substrate of peroxidase and its application to the diagnosis of schistosomiasis. Jpn J Exp Med. 1984 Jun;54(3):131-8. [Content Brief]

[2]. Lauber C, et al. Identification, heterologous expression and characterization of a dye-decolorizing peroxidase of Pleurotus sapidus. AMB Express. 2017 Aug 23;7(1):164. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	1.8226 mL	9.1128 mL	18.2256 mL	45.5639 mL
	5 mM	0.3645 mL	1.8226 mL	3.6451 mL	9.1128 mL
	10 mM	0.1823 mL	0.9113 mL	1.8226 mL	4.5564 mL
	15 mM	0.1215 mL	0.6075 mL	1.2150 mL	3.0376 mL
	20 mM	0.0911 mL	0.4556 mL	0.9113 mL	2.2782 mL
	25 mM	0.0729 mL	0.3645 mL	0.7290 mL	1.8226 mL
	30 mM	0.0608 mL	0.3038 mL	0.6075 mL	1.5188 mL
H₂O	40 mM	0.0456 mL	0.2278 mL	0.4556 mL	1.1391 mL
	50 mM	0.0365 mL	0.1823 mL	0.3645 mL	0.9113 mL
	60 mM	0.0304 mL	0.1519 mL	0.3038 mL	0.7594 mL
	80 mM	0.0228 mL	0.1139 mL	0.2278 mL	0.5695 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

ABTS diammonium salt Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

ABTS diammonium30931-67-0AzBTS-(NH4)2Fluorescent DyeInhibitorinhibitorinhibit

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ABTS diammonium salt (Synonyms: AzBTS-(NH4)2)

Customer Review

ABTS diammonium salt Related Antibodies

2 Publications Citing Use of MCE ABTS diammonium salt

Featured Recommendations

•Related Small Molecules:

•Related Proteins:

Biological Activity

Protocol

Purity & Documentation

References

Customer Review

ABTS diammonium salt Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

ABTS diammonium salt Related Classifications

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Your Recently Viewed Products:

Customer Review

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