1. Metabolic Enzyme/Protease
  2. Phosphatase
  3. AMPPD

AMPPD  (Synonyms: Lumi-Phos Plus; Lumigen PPD; PPD)

Cat. No.: HY-15906 Purity: 98.53%
COA Handling Instructions

AMPPD, a 1,2-dioxo-cyclohexane derivative, is a biochemistry ultrasensitive alkaline phosphatase substrate.

For research use only. We do not sell to patients.

AMPPD Chemical Structure

AMPPD Chemical Structure

CAS No. : 122341-56-4

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in Water
ready for reconstitution
USD 275 In-stock
Solution
10 mM * 1 mL in Water USD 275 In-stock
Solid
1 mg USD 110 In-stock
5 mg USD 250 In-stock
10 mg USD 400 In-stock
25 mg USD 800 In-stock
50 mg USD 1200 In-stock
100 mg USD 1700 In-stock
200 mg   Get quote  
500 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

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Description

AMPPD, a 1,2-dioxo-cyclohexane derivative, is a biochemistry ultrasensitive alkaline phosphatase substrate.

Molecular Weight

382.34

Formula

C18H23O7P

CAS No.
Appearance

Solid

Color

White to off-white

SMILES

COC1(C2=CC(OP(O)(O)=O)=CC=C2)C3(C(C4)CC5CC4CC3C5)OO1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

H2O : 83.33 mg/mL (217.95 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.6155 mL 13.0774 mL 26.1547 mL
5 mM 0.5231 mL 2.6155 mL 5.2309 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 100 mg/mL (261.55 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 98.53%

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 2.6155 mL 13.0774 mL 26.1547 mL 65.3868 mL
5 mM 0.5231 mL 2.6155 mL 5.2309 mL 13.0774 mL
10 mM 0.2615 mL 1.3077 mL 2.6155 mL 6.5387 mL
15 mM 0.1744 mL 0.8718 mL 1.7436 mL 4.3591 mL
20 mM 0.1308 mL 0.6539 mL 1.3077 mL 3.2693 mL
25 mM 0.1046 mL 0.5231 mL 1.0462 mL 2.6155 mL
30 mM 0.0872 mL 0.4359 mL 0.8718 mL 2.1796 mL
40 mM 0.0654 mL 0.3269 mL 0.6539 mL 1.6347 mL
50 mM 0.0523 mL 0.2615 mL 0.5231 mL 1.3077 mL
60 mM 0.0436 mL 0.2180 mL 0.4359 mL 1.0898 mL
80 mM 0.0327 mL 0.1635 mL 0.3269 mL 0.8173 mL
100 mM 0.0262 mL 0.1308 mL 0.2615 mL 0.6539 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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AMPPD Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
AMPPD
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