1. Aminoguanidine hydrochloride

Aminoguanidine hydrochloride  (Synonyms: Pimagedine hydrochloride; GER-11; Aminoguanidinium chloride)

Cat. No.: HY-B1041 Purity: ≥98.0%
COA Handling Instructions

Aminoguanidine hydrochloride (Pimagedine hydrochloride) is an inhibitor of diamine oxidase and nitric oxide synthase. Aminoguanidine hydrochloride has a dose-dependent inhibitory effect on apoptosis induced by Doxorubicin (HY-15142). Aminoguanidine hydrochloride has antioxidant properties. Aminoguanidine hydrochloride can be used in diabetic nephropathy research.

For research use only. We do not sell to patients.

Aminoguanidine hydrochloride Chemical Structure

Aminoguanidine hydrochloride Chemical Structure

CAS No. : 1937-19-5

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Solid + Solvent
10 mM * 1 mL in DMSO
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Solid
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Customer Review

Based on 2 publication(s) in Google Scholar

Other Forms of Aminoguanidine hydrochloride:

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  • Biological Activity

  • Purity & Documentation

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Description

Aminoguanidine hydrochloride (Pimagedine hydrochloride) is an inhibitor of diamine oxidase and nitric oxide synthase. Aminoguanidine hydrochloride has a dose-dependent inhibitory effect on apoptosis induced by Doxorubicin (HY-15142). Aminoguanidine hydrochloride has antioxidant properties. Aminoguanidine hydrochloride can be used in diabetic nephropathy research[1][2][3][4].

In Vitro

Aminoguanidine (100, 200, 500, 1000 μM, 24 h) can reduce DOX-induced DNA damage and apoptosis in A549 cells [1].
Aminoguanidine (100 μM, 30 min) can induce ERK activation in AR42J cells and promote cell proliferation [2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Apoptosis Analysis [1]

Cell Line: A549
Concentration: 100-1000 μM
Incubation Time: 24 h
Result: Showed protective effect on DOX-induced DNA damage and decreased DOX-induced apoptosis.

Cell Proliferation Assay [2]

Cell Line: AR42J
Concentration: 100 μM
Incubation Time: 24-96 h
Result: Showed a significant increase in cell proliferation after incubation for 48 h.
In Vivo

minoguanidine (50 mg/kg, Intraperitoneal injection ) protects mice from CCl4-induced hepatotoxicity[3].
Aminoguanidine (200 mg/kg, Single dose intraperitoneal injection) is protective against cyclophosphamide (CP) -induced oxidative stress and kidney damage in rats[4].br/>

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male Swiss albino mice[3]
Dosage: 50 mg/kg
Administration: Intraperitoneally 30 min before the administration of CCl4
Result: Inhibited the serum AST level and protected hepatotoxin-induced lipid peroxidation.
Animal Model: Adult male Wistar rats[4]
Dosage: 200 mg/kg
Administration: Intraperitoneally 1 hour before the administration of CP and killed 16 hours after CP injection.
Result: Attenuated CP-induced MDA elevation and prevented CP-induced protein oxidation.
Restored the GSH levels and attenuated CP-induced increase in MPO activity.
Clinical Trial
Molecular Weight

110.55

Formula

CH7ClN4

CAS No.
Appearance

Solid

Color

White to off-white

SMILES

NNC(N)=N.[H]Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

H2O : ≥ 100 mg/mL (904.57 mM)

DMSO : 100 mg/mL (904.57 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 9.0457 mL 45.2284 mL 90.4568 mL
5 mM 1.8091 mL 9.0457 mL 18.0914 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

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Volume (start)

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C2

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (22.61 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (22.61 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 100 mg/mL (904.57 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 9.0457 mL 45.2284 mL 90.4568 mL 226.1420 mL
5 mM 1.8091 mL 9.0457 mL 18.0914 mL 45.2284 mL
10 mM 0.9046 mL 4.5228 mL 9.0457 mL 22.6142 mL
15 mM 0.6030 mL 3.0152 mL 6.0305 mL 15.0761 mL
20 mM 0.4523 mL 2.2614 mL 4.5228 mL 11.3071 mL
25 mM 0.3618 mL 1.8091 mL 3.6183 mL 9.0457 mL
30 mM 0.3015 mL 1.5076 mL 3.0152 mL 7.5381 mL
40 mM 0.2261 mL 1.1307 mL 2.2614 mL 5.6536 mL
50 mM 0.1809 mL 0.9046 mL 1.8091 mL 4.5228 mL
60 mM 0.1508 mL 0.7538 mL 1.5076 mL 3.7690 mL
80 mM 0.1131 mL 0.5654 mL 1.1307 mL 2.8268 mL
100 mM 0.0905 mL 0.4523 mL 0.9046 mL 2.2614 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Aminoguanidine hydrochloride
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