Amorolfine hydrochloride (Synonyms: Ro 14-4767/002)

Cat. No.: HY-B0238 Purity: 99.90%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Amorolfine (Ro 14-4767/002) hydrochloride is a potent anti-fungal agent. Amorolfine hydrochloride inhibits ergosterol biosynthesis. Amorolfine hydrochloride has the potential for the research of Neoscytalidium dimidiatum onychomycosis.

For research use only. We do not sell to patients.

Amorolfine hydrochloride Chemical Structure

CAS No. : 78613-38-4

Size	Price	Stock	Quantity

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Solid + Solvent
10 mM * 1 mL in DMSO ready for reconstitution	USD 117	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 117	In-stock	Estimated Time of Arrival: December 31
Solid
100 mg	USD 106	In-stock	Estimated Time of Arrival: December 31
200 mg	USD 198	In-stock	Estimated Time of Arrival: December 31
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Biological Activity
Purity & Documentation
References
Customer Review

Description

In Vitro

Amorolfine hydrochloride acts primarily by inhibiting ergosterol biosynthesis, a component of fungal cell membrane, and possesses both fungistatic and fungicidal activity^[1].
Amorolfine hydrochloride (0-128 mg/l) (2-7 days) shows anti-fungal activity for Dermatophyte fungi, Filamentous fungi, Pathogenic yeasts, Dimorphic fungi, Dematiaceous fungi^[1].
Amorolfine hydrochloride shows anti-fungal activity with 90% killing corresponding concentrations of 3, 3, 10 and 100 mg/L for Trichophyton mentagrophytes, C. albicans, Histoplasma capsulatum, Cryptococcus neoformans, respectively^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Amorolfine hydrochloride (0.01%-1%; Intravaginal application; twice daily for 3 days) shows anti-fungal activity in rats with vaginal candidiasis^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Rats (vaginal candidiasis)^[1]
Dosage:	0.01%-1%
Administration:	Intravaginal application; twice daily for 3 days
Result:	Produced a dose dependent log reduction in cell count; a concentration of 1 % cleared the vagina of C. albicans completely.

Clinical Trial

Molecular Weight

353.97

Formula

C₂₁H₃₆ClNO

CAS No.

78613-38-4

Appearance

Solid

Color

White to off-white

SMILES

C[C@@H]1O[C@H](C)CN(CC(C)CC2=CC=C(C(C)(C)CC)C=C2)C1.Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 12.5 mg/mL (35.31 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 3.33 mg/mL (9.41 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.8251 mL	14.1255 mL	28.2510 mL
5 mM	0.5650 mL	2.8251 mL	5.6502 mL
10 mM	0.2825 mL	1.4125 mL	2.8251 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.25 mg/mL (3.53 mM); Clear solution

This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1.25 mg/mL (3.53 mM); Clear solution

This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 1.25 mg/mL (3.53 mM); Clear solution

This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.90%

Select Batch:

Data Sheet (272 KB) SDS (536 KB)

COA (244 KB) LCMS (260 KB)

Handling Instructions (2659 KB)

References

[1]. Haria M, et al. Amorolfine. A review of its pharmacological properties and therapeutic potential in the treatment of onychomycosis and other superficial fungal infections. Drugs. 1995 Jan;49(1):103-20. [Content Brief]

[2]. Bunyaratavej S, et al. Efficacy of 5% amorolfine nail lacquer in Neoscytalidium dimidiatum onychomycosis. J Dermatolog Treat. 2016 Aug;27(4):359-63. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	2.8251 mL	14.1255 mL	28.2510 mL	70.6275 mL
H₂O / DMSO	5 mM	0.5650 mL	2.8251 mL	5.6502 mL	14.1255 mL
DMSO	10 mM	0.2825 mL	1.4125 mL	2.8251 mL	7.0627 mL
	15 mM	0.1883 mL	0.9417 mL	1.8834 mL	4.7085 mL
	20 mM	0.1413 mL	0.7063 mL	1.4125 mL	3.5314 mL
	25 mM	0.1130 mL	0.5650 mL	1.1300 mL	2.8251 mL
	30 mM	0.0942 mL	0.4708 mL	0.9417 mL	2.3542 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Amorolfine hydrochloride Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Amorolfine78613-38-4Ro 14-4767/002FungalAntibioticanti-fungalvaginal candidiasisonychomycosisInhibitorinhibitorinhibit

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