2. Anti-infection Cell Cycle/DNA Damage Stem Cell/Wnt Autophagy Metabolic Enzyme/Protease
  3. SARS-CoV Casein Kinase Autophagy 11β-HSD
  4. Emodin

Emodin  (Synonyms: Frangula emodin)

Cat. No.: HY-14393 Purity: 99.20%
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Emodin (Frangula emodin), an anthraquinone derivative, is an anti-SARS-CoV compound. Emodin blocks the SARS coronavirus spike protein and angiotensin-converting enzyme 2 (ACE2) interaction. Emodin inhibits casein kinase-2 (CK2). Anti-inflammatory and anticancer effects. Emodin is a potent selective 11β-HSD1 inhibitor with the IC50 of 186 and 86 nM for human and mouse 11β-HSD1, respectively. Emodin ameliorates metabolic disorder in diet-induced obese mice.

For research use only. We do not sell to patients.

Emodin Chemical Structure

Emodin Chemical Structure

CAS No. : 518-82-1

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Based on 19 publication(s) in Google Scholar

Other Forms of Emodin:

Emodin Related Antibodies

Top Publications Citing Use of Products

    Emodin purchased from MedChemExpress. Usage Cited in: Am J Transl Res. 2020 May 15;12(5):1851-1861.  [Abstract]

    Emodin resensitizes SW480/5-Fu cells to 5-Fu. SW480/5-Fu cells are treated with 5-Fu or/and Emodin for 72 h. The proliferation of SW480/5-Fu cell is detected by Ki67 immunofluorescence.

    Emodin purchased from MedChemExpress. Usage Cited in: Am J Transl Res. 2020 May 15;12(5):1851-1861.  [Abstract]

    Emodin reverses 5-Fu chemoresistance in CRC via downregulation of PI3K/Akt signaling pathway. SW480/5-Fu cells are treated with 5-Fu or/and Emodin for 72 h. Then, the protein expressions of Bax, Bcl-2, cleaved caspase3, ERK, Akt, p-ERK and p-Akt in SW480/5-Fu cells are investigated by Western-blot.

    View All Casein Kinase Isoform Specific Products:

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    CK1 CK2

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Emodin (Frangula emodin), an anthraquinone derivative, is an anti-SARS-CoV compound. Emodin blocks the SARS coronavirus spike protein and angiotensin-converting enzyme 2 (ACE2) interaction[1]. Emodin inhibits casein kinase-2 (CK2). Anti-inflammatory and anticancer effects[2]. Emodin is a potent selective 11β-HSD1 inhibitor with the IC50 of 186 and 86 nM for human and mouse 11β-HSD1, respectively. Emodin ameliorates metabolic disorder in diet-induced obese mice[3].

    IC50 & Target[1][2][3]

    SARS-CoV

     

    CK2α Wild-type

    1.4 μM (IC50, at ATP concentration is 10 μM)

    CK2α Wild-type

    5.9 μM (IC50, at ATP concentration is 50 μM)

    mouse 11β-HSD1

    86 nM (IC50)

    human 11β-HSD1

    186 nM (IC50)

    In Vitro

    Emodin (10-400 μM) blocks the binding of S protein to ACE2 in a dose-dependent manner with the IC50 value of 200 μM[1].
    Emodin (5-50 μM) inhibits the S protein-pseudotyped retrovirus infectivity in a dose-dependent manner. Emodin blocks the SARS-CoV S protein binding to Vero E6 cells[1].
    Emodin inhibits casein kinase-2 (CK2) with IC50s of 5.9, 30.0, and 7.1 μM for CK2α Wild-type, Ile174Ala mutant, and His160Ala mutant at ATP concentration is 50 μM, respectively. The IC50s are 1.40 and 38.00 μM for CK2α Wild-type, and Val66Ala mutant at ATP concentration is 10 μM[2].
    Emodin exhibits low inhibitory activity against mouse and human 11β-hydroxysteroid dehydrogenase type 2 (11β-HSD2), with an IC50 higher than 1 mM, indicating that Emodin is more than 5000-fold selective for the human and mouse 11β-HSD1 enzymes over the type 2 isoenzyme[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Emodin Related Antibodies

    Cell Viability Assay[1]

    Cell Line: Vero E6 cells transfected with the plasmid encoding ACE2
    Concentration: 0, 5, 25, 50 μM
    Incubation Time: 24 hours
    Result: Vero cells treated with 50 μM remained 82.4±3.8% viability, the anti-SARS-CoV activity was not due to toxicity.
    In Vivo

    Emodin (single oral administration of 100 or 200 mg/kg) inhibits 11β-HSD1 activity in normal C57BL/6J male mice[3].
    Emodin (100 mg/kg; oral administration; b.i.d.) improves insulin sensitivity and lipid metabolism, and lowers blood glucose and hepatic PEPCK, and glucose-6-phosphatase mRNA in diet-induced obese (DIO) mice[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: C57BL/6J male mice[3]
    Dosage: 100 or 200 mg/kg
    Administration: Acute administered p.o. ; Two hours later, the mice were killed by cervical dislocation,
    Result: Significantly inhibited liver 11β-HSD1 enzymatic activity by 17.6 and 31.3% and mesenteric fat 11β-HSD1 enzymatic activity by 21.5 and 46.7% at 100 or 200 mg/kg, respectively.
    Animal Model: DIO mice (C57BL/6J male mice were fed a formulated research diet)[3]
    Dosage: 100 mg/kg
    Administration: Oral gavage; twice per day; for 35 days
    Result: Reduced fasting glucose concentrations to 77.2% of the vehicle control mice after 7 days of treatment, and these remained significantly lower throughout the treatment period.
    Exhibited a significant reduction in blood glucose levels at all time-points following oral glucose challenge after 24 days of treatment.
    Evoked a significantly greater reduction in blood glucose values 40 and 90 min after insulin injection after 28 days of treatment.
    The serum insulin level was also significantly reduced, to 66.2% of control mice, after 35 days of treatment.
    Improved the lipid profiles. The serum triglyceride and total cholesterol levels were significantly reduced by 19.3 and 12.5% after 35 days of treatment, respectively.
    Caused a 22.7% reduction of non-esterified free fatty acid (NEFA) level.
    Lowered body weight and appetite from day 18 of the treatment; their body weights were reduced by 13.9% at the end of treatment.
    Clinical Trial
    Molecular Weight

    270.24

    Formula

    C15H10O5
    CAS No.
    Appearance

    Solid

    Color

    Yellow to orange

    SMILES

    O=C1C2=C(C=C(C)C=C2O)C(C3=CC(O)=CC(O)=C31)=O
    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 1 year
    -20°C 6 months
    Solvent & Solubility
    In Vitro: 

    Acetone : 10.87 mg/mL (40.22 mM; Need ultrasonic)

    DMSO : 5.41 mg/mL (20.02 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Ethanol : < 1 mg/mL (insoluble)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.7004 mL 18.5021 mL 37.0041 mL
    5 mM 0.7401 mL 3.7004 mL 7.4008 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
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    Concentration
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    Volume
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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

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    Volume (start)

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    Concentration (final)

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    In Vivo:

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  0.5% CMC-Na/saline water

      Solubility: 3.33 mg/mL (12.32 mM); Suspened solution; Need ultrasonic

    • Protocol 2

      Add each solvent one by one:  0.5% MC  0.5% Tween-80

      Solubility: 10 mg/mL (37.00 mM); Suspended solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    Purity & Documentation

    Purity: 99.20%

    COA (249 KB) HNMR (246 KB) LCMS (256 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO / Acetone 1 mM 3.7004 mL 18.5021 mL 37.0041 mL 92.5104 mL
    5 mM 0.7401 mL 3.7004 mL 7.4008 mL 18.5021 mL
    10 mM 0.3700 mL 1.8502 mL 3.7004 mL 9.2510 mL
    15 mM 0.2467 mL 1.2335 mL 2.4669 mL 6.1674 mL
    20 mM 0.1850 mL 0.9251 mL 1.8502 mL 4.6255 mL
    Acetone 25 mM 0.1480 mL 0.7401 mL 1.4802 mL 3.7004 mL
    30 mM 0.1233 mL 0.6167 mL 1.2335 mL 3.0837 mL
    40 mM 0.0925 mL 0.4626 mL 0.9251 mL 2.3128 mL
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    Emodin Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Emodin518-82-1Frangula emodinSARS-CoVCasein KinaseAutophagy11β-HSDSARS coronavirus11beta-HSD11β-hydroxysteroid dehydrogenases11 beta-hydroxysteroid dehydrogenasemiceinsulinresistancemetabolicsyndromediabeteSARScoronavirusSpikeproteincaseinkinaseCK211β-HSD1Inhibitorinhibitorinhibit

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