Hematoporphyrin (Synonyms: Hematoporphyrin IX)

Cat. No.: HY-B0754 Purity: 95.81%: FAQs
Data Sheet SDS COA Handling Instructions

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Hematoporphyrin (Hematoporphyrin IX), a photosensitizer, is a substrate for affinity chromatography of heme-binding proteins. Hematoporphyrin can induce apoptosis in U87 glioma cells and decrease tumor growth in vivo when exposed to red light.

The free form of the compound is prone to instability, it is advisable to consider the stable salt form (Hematoporphyrin dihydrochloride) that retains the same biological activity.

For research use only. We do not sell to patients.

Hematoporphyrin Chemical Structure

CAS No. : 14459-29-1

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100 mg	USD 72	Ask For Quote & Lead Time

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Other In-stock Forms of Hematoporphyrin:

Other Forms of Hematoporphyrin:

Hematoporphyrin dihydrochloride In-stock

11 Publications Citing Use of MCE Hematoporphyrin

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Biological Activity
Purity & Documentation
References
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Description

IC₅₀ & Target

Human Endogenous Metabolite

In Vitro

Hematoporphyrin (20-120 nM; 60 min) dose-dependently inhibits cell viability in U87 and U251 glioma cells, with IC₅₀s of 85 and 166 nM, respectively^[2].
Hematoporphyrin (85 nM; 60 min) induces cell apoptosis via induction of ROS in U87 cells^[2].
Hematoporphyrin (85 nM; 60 min) induces morphological changes of U87 cells under the red light, including shrinking, fragmentation^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[2]

Cell Line:	U87 and U251 cells
Concentration:	20, 40, 60, 80, 100, 120 nM
Incubation Time:	60 min
Result:	Inhibited cell viability in a dose-dependent manner. Was more effective under the red light than white light.

Apoptosis Analysis^[2]

Cell Line:	U87 cells
Concentration:	85 nM
Incubation Time:	60 min
Result:	Induced apoptotic nuclei in U87 cells with low cell density. Induced the ROS and decreased the mitochondrial membrane potential.

In Vivo

Hematoporphyrin (5-10 mg/kg; i.p. for 2 months) with the irradiation of red light rapidly decreases the tumor size of rats, due to necrosis caused both by direct action of the photoactivated porphyrin on the tumor cells and by secondary effects on blood vessels^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Wistar albino rats of both sexes (20 d; 60-80 g) bearing a subcutaneous solid Yoshida hepatoma AH-130^[3]
Dosage:	5, 10 mg/kg
Administration:	I.p. daily during the initial 10 days and biweekly for the next 2 months
Result:	No tumor could be palpated a few days after exposure of the rats to light. The skin healed completely and regrowth of the hair occurred. Massive coagulation necrosis of the tumor 24 h after phototreatment (×40).

Clinical Trial

Molecular Weight

598.69

Formula

C₃₄H₃₈N₄O₆

CAS No.

14459-29-1

Appearance

Solid

Color

Pale purple to purple

SMILES

O=C(O)CCC1=C2/C=C3C(CCC(O)=O)=C(C)C(/C=C(N/4)/C(C)=C(C(O)C)C4=C\C5=N/C(C(C(O)C)=C5C)=C\C(N2)=C1C)=N/3

Structure Classification

Others

Initial Source

Endogenous metabolite

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light

*The compound is unstable in solutions, freshly prepared is recommended.

Solvent & Solubility

In Vitro:

DMSO : 150 mg/mL (250.55 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.6703 mL	8.3516 mL	16.7031 mL
5 mM	0.3341 mL	1.6703 mL	3.3406 mL
10 mM	0.1670 mL	0.8352 mL	1.6703 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (4.18 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (4.18 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*The compound is unstable in solutions, freshly prepared is recommended.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: ≥98.0%

Select Batch:

Data Sheet (288 KB) SDS (252 KB)

COA (263 KB) HNMR (197 KB)

Handling Instructions (2659 KB)

References

[1]. Olsen, K.W., Affinity chromatography of heme-binding proteins: synthesis and characterization of hematin- and hematoporphyrin-agarose. Methods Enzymol, 1986. 123: p. 324-31. [Content Brief]

[2]. Yuan SX, et, al. Underlying mechanism of the photodynamic activity of hematoporphyrin‑induced apoptosis in U87 glioma cells. Int J Mol Med. 2018 Apr;41(4):2288-2296. [Content Brief]

[3]. Tomio L, et, al. Effect of hematoporphyrin and red light on AH-130 solid tumors in rats. Acta Radiol Oncol. 1983;22(1):49-53. [Content Brief]

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.6703 mL	8.3516 mL	16.7031 mL	41.7578 mL
	5 mM	0.3341 mL	1.6703 mL	3.3406 mL	8.3516 mL
	10 mM	0.1670 mL	0.8352 mL	1.6703 mL	4.1758 mL
	15 mM	0.1114 mL	0.5568 mL	1.1135 mL	2.7839 mL
	20 mM	0.0835 mL	0.4176 mL	0.8352 mL	2.0879 mL
	25 mM	0.0668 mL	0.3341 mL	0.6681 mL	1.6703 mL
	30 mM	0.0557 mL	0.2784 mL	0.5568 mL	1.3919 mL
	40 mM	0.0418 mL	0.2088 mL	0.4176 mL	1.0439 mL
	50 mM	0.0334 mL	0.1670 mL	0.3341 mL	0.8352 mL
	60 mM	0.0278 mL	0.1392 mL	0.2784 mL	0.6960 mL
	80 mM	0.0209 mL	0.1044 mL	0.2088 mL	0.5220 mL
	100 mM	0.0167 mL	0.0835 mL	0.1670 mL	0.4176 mL

Hematoporphyrin Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Hematoporphyrin14459-29-1Hematoporphyrin IXApoptosisEndogenous MetabolitephotosensitizerapoptosisgliomatumorredlightInhibitorinhibitorinhibit

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