2. Neuronal Signaling Autophagy
  3. CaMK Autophagy
  4. KN-93 phosphate

KN-93 phosphate is a novel membrane-permeant synthetic inhibitor of purified neuronal CaMK-II, with Ki of 370 nM.

For research use only. We do not sell to patients.

KN-93 phosphate Chemical Structure

KN-93 phosphate Chemical Structure

CAS No. : 1913269-12-1

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 174 In-stock
Solution
10 mM * 1 mL in DMSO USD 174 In-stock
Solid
1 mg USD 66 In-stock
5 mg USD 132 In-stock
10 mg USD 180 In-stock
50 mg USD 720 In-stock
100 mg   Get quote  
200 mg   Get quote  

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Customer Review

Based on 58 publication(s) in Google Scholar

Other Forms of KN-93 phosphate:

KN-93 phosphate Related Antibodies

Top Publications Citing Use of Products

54 Publications Citing Use of MCE KN-93 phosphate

WB

    KN-93 phosphate purchased from MedChemExpress. Usage Cited in: Acta Pharmacol Sin. 2020 Feb;41(2):218-228.   [Abstract]

    HUVECs are pretreated with KN93 for 1 h, followed by ZYZ-803 (1 μM) for 30 min. Protein levels are assessed by Western blot.

    KN-93 phosphate purchased from MedChemExpress. Usage Cited in: Diabetes. 2018 Sep;67(9):1748-1760.  [Abstract]

    Immunoblotting analysis of PCK1, G6Pase, and phospho-CaMKIIγ proteins in NTG and HCTG hepatocytes in response to PGF2α and in the presence or absence of KN-93 treatment.

    KN-93 phosphate purchased from MedChemExpress. Usage Cited in: Front Pharmacol. 2018 Apr 12;9:362.  [Abstract]

    MK 733-increased biotinylated α7nAChR protein is blocked by the addition of KN93 (P<0.01, n=6 mice).

    KN-93 phosphate purchased from MedChemExpress. Usage Cited in: Exp Cell Res. 2018 Mar 15;364(2):198-207.  [Abstract]

    Western blot analysis of β-catenin protein level in HEK 293T cells stimulated for 12h with rAGR2 (100 ng/mL) coupled with or without KN93 (10 μM).

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    KN-93 phosphate is a novel membrane-permeant synthetic inhibitor of purified neuronal CaMK-II, with Ki of 370 nM.

    IC50 & Target

    CaMK II

     

    In Vitro

    After 2 days of KN-93 treatment, 95% of cells are arrested in G1. G1 arrest is reversible; 1 day after KN-93 release, a peak of cells had progressed into S and G2-M. KN-93 also blocks cell growth stimulated by basic fibroblast growth factor, platelet-derived growth factor-BB, and epidermal growth factor in NIH 3T3 fibroblasts[1]. KN-93 inhibits the H+, K+-ATPase activity but strongly dissipates the proton gradient formed in the gastric membrane vesicles and reduces the volume of luminal space[2]. KN-93 (0.5 μM) prevents increased LV developed pressure during action potential prolongation and early afterdepolarizations. Ca2+-independent CaM kinase activity is increased during early afterdepolarizations and this increase is prevented by KN-93[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    KN-93 phosphate Related Antibodies
    Molecular Weight

    599.03

    Formula

    C26H32ClN2O8PS
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    OP(O)(O)=O.O=S(C1=CC=C(OC)C=C1)(N(C2=CC=CC=C2CN(C/C=C/C3=CC=C(Cl)C=C3)C)CCO)=O
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture)

    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (166.94 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : 50 mg/mL (83.47 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.6694 mL 8.3468 mL 16.6937 mL
    5 mM 0.3339 mL 1.6694 mL 3.3387 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

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    Volume (start)

    V1

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    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 10 mg/mL (16.69 mM); Clear solution

      This protocol yields a clear solution of ≥ 10 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (100.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 10 mg/mL (16.69 mM); Clear solution

      This protocol yields a clear solution of ≥ 10 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (100.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

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    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 1.6694 mL 8.3468 mL 16.6937 mL 41.7341 mL
    5 mM 0.3339 mL 1.6694 mL 3.3387 mL 8.3468 mL
    10 mM 0.1669 mL 0.8347 mL 1.6694 mL 4.1734 mL
    15 mM 0.1113 mL 0.5565 mL 1.1129 mL 2.7823 mL
    20 mM 0.0835 mL 0.4173 mL 0.8347 mL 2.0867 mL
    25 mM 0.0668 mL 0.3339 mL 0.6677 mL 1.6694 mL
    30 mM 0.0556 mL 0.2782 mL 0.5565 mL 1.3911 mL
    40 mM 0.0417 mL 0.2087 mL 0.4173 mL 1.0434 mL
    50 mM 0.0334 mL 0.1669 mL 0.3339 mL 0.8347 mL
    60 mM 0.0278 mL 0.1391 mL 0.2782 mL 0.6956 mL
    80 mM 0.0209 mL 0.1043 mL 0.2087 mL 0.5217 mL
    DMSO 100 mM 0.0167 mL 0.0835 mL 0.1669 mL 0.4173 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    KN-93 phosphate Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    KN-931913269-12-1KN93KN 93CaMKAutophagyCalmodulin-dependent protein kinasesCalmodulin-dependent kinasesInhibitorinhibitorinhibit

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