NOD-IN-1

Name: NOD-IN-1
Brand: MedChemExpress
SKU: HY-100691
Rating: 5.0 (9 reviews)

Cat. No.: HY-100691 Purity: 99.70%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

NOD-IN-1 is a potent mixed inhibitor of nucleotide-binding oligomerization domain (NOD)-like receptors, NOD1 and NOD2, with IC₅₀ of 5.74 μM and 6.45 μM, respectively.

For research use only. We do not sell to patients.

NOD-IN-1 Chemical Structure

CAS No. : 132819-92-2

Size	Price	Stock	Quantity
Solid + Solvent
10 mM * 1 mL in DMSO ready for reconstitution	USD 124	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 124	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	USD 88	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 165	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 275	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 858	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 1210	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 9 publication(s) in Google Scholar

7 Publications Citing Use of MCE NOD-IN-1

•Research Square Print. December 14th, 2022.

: NOD-IN-1 purchased from MedChemExpress. Usage Cited in: J Hazard Mater. 2021 Jan 15;402:123527. [Abstract]; Under the condition of DBP and DEHP exposure alone, the protein levels of Bax and Caspase9 are significantly increased, and the increased levels are more significantly after DBP and DEHP exposure together. But, in DBP + NOD2- group, DEHP + NOD2- group and DBP + DEHP + NOD2- group, the increase levels of Bax and Caspase9 have been significantly alleviated, respectively.

: NOD-IN-1 purchased from MedChemExpress. Usage Cited in: Front Cell Infect Microbiol. 2020 May 5;10:196. [Abstract]; The western blotting shows that the protein levels of NLRP3, caspase-1, GSDMD, IL-1β, IL-18 are decreased in NOD-IN-1 group compared with H3 group.

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Description

NOD-IN-1 is a potent mixed inhibitor of nucleotide-binding oligomerization domain (NOD)-like receptors, NOD1 and NOD2, with IC₅₀ of 5.74 μM and 6.45 μM, respectively.

IC₅₀ & Target

NOD1

NOD2

In Vitro

NOD-IN-1 (compound 4) is potent mixed inhibitor of NOD1 and NOD2, displaying a balanced inhibitory activity on both targets in the low micromolar range. NOD-IN-1 (IC₅₀ (NOD1)=5.74 μM; IC₅₀ (NOD2)=6.45 μM) is identified as the best of the series, possessing NOD1- and NOD2-inhibitory activities in the lower micromolar range. These results show that NOD-IN-1 is 7-fold less potent than Noditinib-1 in terms of NOD1 inhibition and completely devoid of selective activity for NOD1 or NOD2 as opposed to Noditinib-1. NOD-IN-1 exhibits balanced dual activities of less than 10 μM on the two targets^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

343.40

Formula

C₁₈H₁₇NO₄S

CAS No.

132819-92-2

Appearance

Solid

Color

White to off-white

SMILES

O=S(N1C(C(OCC)=O)=CC2=CC=CC=C21)(C3=CC=C(C)C=C3)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (291.21 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.9121 mL	14.5603 mL	29.1206 mL
5 mM	0.5824 mL	2.9121 mL	5.8241 mL
10 mM	0.2912 mL	1.4560 mL	2.9121 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.28 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (7.28 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (7.28 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.70%

Select Batch:

Data Sheet (285 KB) SDS (392 KB)

COA (264 KB) LCMS (266 KB)

Handling Instructions (2659 KB)

References

[1]. Kecek Plesec K, et al. Identification of indole scaffold-based dual inhibitors of NOD1 and NOD2. Bioorg Med Chem. 2016 Nov 1;24(21):5221-5234. [Content Brief]

Cell Assay
^[1]

An MTS assay in which the proliferation rates of HEK-Blue NOD1 cells are measured in the presence of Noditinib-1 and of the synthesized potential NOD1 inhibitor NOD-IN-1 is employed to screen these compounds for potential cytotoxicity. Cells are treated for 24 h with the compound of interest at concentrations of up to 25 μM. Comparison of the resulting metabolic activities with that of the untreated control showed that all compounds are well tolerated by HEK-Blue NOD1 cells, since their residual metabolic activities do not fall below 80% at the maximum concentration tested^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

[1]. Kecek Plesec K, et al. Identification of indole scaffold-based dual inhibitors of NOD1 and NOD2. Bioorg Med Chem. 2016 Nov 1;24(21):5221-5234. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.9121 mL	14.5603 mL	29.1206 mL	72.8014 mL
	5 mM	0.5824 mL	2.9121 mL	5.8241 mL	14.5603 mL
	10 mM	0.2912 mL	1.4560 mL	2.9121 mL	7.2801 mL
	15 mM	0.1941 mL	0.9707 mL	1.9414 mL	4.8534 mL
	20 mM	0.1456 mL	0.7280 mL	1.4560 mL	3.6401 mL
	25 mM	0.1165 mL	0.5824 mL	1.1648 mL	2.9121 mL
	30 mM	0.0971 mL	0.4853 mL	0.9707 mL	2.4267 mL
	40 mM	0.0728 mL	0.3640 mL	0.7280 mL	1.8200 mL
	50 mM	0.0582 mL	0.2912 mL	0.5824 mL	1.4560 mL
	60 mM	0.0485 mL	0.2427 mL	0.4853 mL	1.2134 mL
	80 mM	0.0364 mL	0.1820 mL	0.3640 mL	0.9100 mL
	100 mM	0.0291 mL	0.1456 mL	0.2912 mL	0.7280 mL

NOD-IN-1 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

NOD-IN-1132819-92-2NOD-like Receptor (NLR)Inhibitorinhibitorinhibit

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NOD-IN-1

Customer Review

NOD-IN-1 Related Antibodies

7 Publications Citing Use of MCE NOD-IN-1

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•Related Proteins:

Biological Activity

Protocol

Purity & Documentation

References

Customer Review

NOD-IN-1 Related Antibodies

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Complete Stock Solution Preparation Table

NOD-IN-1 Related Classifications

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