2. GPCR/G Protein Neuronal Signaling Stem Cell/Wnt MAPK/ERK Pathway
  3. Adrenergic Receptor ERK
  4. Salbutamol

Salbutamol  (Synonyms: Albuterol; AH-3365)

Cat. No.: HY-B1037 Purity: 99.92%
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Salbutamol (Albuterol) is a short-acting beta-2 adrenergic receptor agonist with oral activity. Salbutamol promotes tumorigenesis of gastric cancer cells through the β2-AR/ERK/EMT pathway. Salbutamol is used to study bronchospasms caused by asthma and chronic obstructive pulmonary disease (COPD).

For research use only. We do not sell to patients.

Salbutamol Chemical Structure

Salbutamol Chemical Structure

CAS No. : 18559-94-9

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Based on 2 publication(s) in Google Scholar

Other Forms of Salbutamol:

Salbutamol Related Antibodies

Top Publications Citing Use of Products

View All Adrenergic Receptor Isoform Specific Products:

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α adrenergic receptor β adrenergic receptor

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ERK ERK1 ERK2 ERK5 ERK8

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Salbutamol (Albuterol) is a short-acting beta-2 adrenergic receptor agonist with oral activity. Salbutamol promotes tumorigenesis of gastric cancer cells through the β2-AR/ERK/EMT pathway. Salbutamol is used to study bronchospasms caused by asthma and chronic obstructive pulmonary disease (COPD)[1][2].

IC50 & Target

β adrenergic receptor

 

In Vitro

Salbutamol (16 μM, 24 h) significantly induces EMT, migration and invasion by ERK (extracellular signal-regulated kinase) phosphorylation in gastric cancer cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Salbutamol Related Antibodies

Western Blot Analysis[1]

Cell Line: MGC803, SGC7901
Concentration: 16 μM
Incubation Time: 24 h
Result: Increased β2-AR and ERK levels.
Upregulated mesenchymal markers, CDH2 (N-cadherin) and Snail expression, and decreased the expression of epithelial marker CDH1 (E-cadherin).
In Vivo

Salbutamol (5 mg/kg/day, orally, for 2 consecutive weeks) can increase tumor growth in nude mice with gastric cance[1].
Salbutamol (6 mg/kg orally for 4 weeks) has a significant protective effect against STZ (HY-13753)-induced skeletal muscle atrophy in diabetic rats[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: STZ induced diabetic rats[2]
Dosage: 6 mg/kg
Administration: p.o. for 4 weeks
Result: Enhanced muscle grip strength, coordination, and antioxidant levels.
Reduced proinflammatory markers and oxidative stress.
Reduced serum muscle biomarkers.
Increased testosterone, restored lipidemic levels, and improved muscle cellular architecture.
Clinical Trial
Molecular Weight

239.31

Formula

C13H21NO3
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

OC(CNC(C)(C)C)C1=CC=C(O)C(CO)=C1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (417.87 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.1787 mL 20.8934 mL 41.7868 mL
5 mM 0.8357 mL 4.1787 mL 8.3574 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (10.45 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (10.45 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 4.1787 mL 20.8934 mL 41.7868 mL 104.4670 mL
5 mM 0.8357 mL 4.1787 mL 8.3574 mL 20.8934 mL
10 mM 0.4179 mL 2.0893 mL 4.1787 mL 10.4467 mL
15 mM 0.2786 mL 1.3929 mL 2.7858 mL 6.9645 mL
20 mM 0.2089 mL 1.0447 mL 2.0893 mL 5.2234 mL
25 mM 0.1671 mL 0.8357 mL 1.6715 mL 4.1787 mL
30 mM 0.1393 mL 0.6964 mL 1.3929 mL 3.4822 mL
40 mM 0.1045 mL 0.5223 mL 1.0447 mL 2.6117 mL
50 mM 0.0836 mL 0.4179 mL 0.8357 mL 2.0893 mL
60 mM 0.0696 mL 0.3482 mL 0.6964 mL 1.7411 mL
80 mM 0.0522 mL 0.2612 mL 0.5223 mL 1.3058 mL
100 mM 0.0418 mL 0.2089 mL 0.4179 mL 1.0447 mL
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Salbutamol Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Salbutamol18559-94-9Albuterol AH-3365AH3365AH 3365AH-3365Adrenergic ReceptorERKBeta ReceptorExtracellular signal regulated kinasesβ2-ARAgonistMGC803SGC7901COPDInhibitorinhibitorinhibit

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