Sodium orthovanadate

Name: Sodium orthovanadate
Brand: MedChemExpress
SKU: HY-D0852
Rating: 5.0 (7 reviews)

Cat. No.: HY-D0852 Purity: ≥99.0%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Sodium orthovanadate is an inhibitor of protein tyrosine phosphatases, alkaline phosphatases and a number of ATPases, most likely acting as a phosphate analogue.

For research use only. We do not sell to patients.

Sodium orthovanadate Chemical Structure

CAS No. : 13721-39-6

Size	Price	Stock	Quantity
Solid + Solvent
10 mM * 1 mL in Water ready for reconstitution	USD 55	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in Water	USD 55	In-stock	Estimated Time of Arrival: December 31
Solid
500 mg	USD 50	In-stock	Estimated Time of Arrival: December 31
5 g	USD 60	In-stock	Estimated Time of Arrival: December 31
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Customer Review

Based on 7 publication(s) in Google Scholar

5 Publications Citing Use of MCE Sodium orthovanadate

Cell Viability Assay

Proliferation Assay

: Sodium orthovanadate purchased from MedChemExpress. Usage Cited in: Tissue Cell. 2023 May 18, 102109.; Sodium orthovanadate (SOV; 0, 2, 4, 8, 16, 32 µM; 48 h) significantly suppresses the proliferative capacity of SGC-7901 and MGC-803 in a dose-dependent manner.

: Sodium orthovanadate purchased from MedChemExpress. Usage Cited in: Tissue Cell. 2023 May 18, 102109.; Sodium orthovanadate (SOV; 0, 2, 4, 8, 16, 32 µM; 48 h) significantly suppresses the cell viability of SGC-7901 and MGC-803 in a dose-dependent manner.

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Purity & Documentation
References
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Description

Sodium orthovanadate is an inhibitor of protein tyrosine phosphatases, alkaline phosphatases and a number of ATPases, most likely acting as a phosphate analogue.

IC₅₀ & Target

PPTPase^[1].

In Vitro

In the presence of oxidizing agents vanadium ions exist as the hydrated monomer of Sodium orthovanadate (vanadate: HVO4^2- or H₂VO^4-) at micromolar concentations near neutral pH. Sodium orthovanadate (vanadate) also begins to polymerize at concentrations greater than 0.1 mM at neutral pH. The yellow-orange solutions of decavanadate can be converted to the colorless solutions of monomeric Sodium orthovanadate (vanadate) by dilution after a period of many hours. The process is hastened by boiling at pH 10, which encourages the kinetically sluggish depolymerization process^[1]. Sodium orthovanadate could alter the phosphorylation status of ASK1 at serine 83 and threonine 845 induced by ischemia. Sodium orthovanadate could increase the tyrosine posphorylation of PTEN and further inhibit the activation of ASK1 via activating Akt during cerebral ischemia^[2].

Sodium orthovanadate needs to be fully activated (depolymerized) to obtain maximum phosphatase inhibitory activity. The steps are as follows:
1. Dissolve sodium orthovanadate in water to prepare a 200mM solution. Weigh an appropriate amount of sodium orthovanadate powder and dissolve it in pure water.
2. Adjust pH to 10.0 with 1N NaOH or 1N HCl. At this time the solution appears yellow.
3. Heat and boil until the solution becomes colorless (boil for about 10 minutes), and all crystals must be fully dissolved.
4. Cool to room temperature.
5. Readjust the pH to 10.0 and repeat steps 3 and 4 until the solution remains colorless and the pH stabilizes at 10.0.
6. Divide the activated sodium orthovanadate solution into small portions (such as 1mL) and store at -20°C. The storage solution can be added directly to the cell or tissue lysis solution and diluted to a working solution such as 1 mM. Cell culture experiments need to be filtered and sterilized with a 0.2μm filter, and then diluted and added to the culture medium;
7. After taking out the aliquoted sample from -20℃ and melting it, the solution needs to be heated to about 90~100℃, vortex and mix well to fully dissolve the crystals.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

183.91

Formula

Na₃O₄V

CAS No.

13721-39-6

Appearance

Solid

Color

Off-white to light yellow

SMILES

[O].[Na].[V]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

H₂O : 8.33 mg/mL (45.29 mM; Need ultrasonic)

DMSO : < 1 mg/mL (insoluble or slightly soluble)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	5.4374 mL	27.1872 mL	54.3744 mL
5 mM	1.0875 mL	5.4374 mL	10.8749 mL
10 mM	0.5437 mL	2.7187 mL	5.4374 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 16.67 mg/mL (90.64 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: ≥99.0%

Select Batch:

Data Sheet (275 KB) SDS (392 KB)

COA (306 KB)

Handling Instructions (2659 KB)

References

[1]. Gordon JA, et al. Use of vanadate as protein-phosphotyrosine phosphatase inhibitor. Methods Enzymol. 1991;201:477-82. [Content Brief]

[2]. Wu DN, et al. Down-regulation of PTEN by sodium orthovanadate inhibits ASK1 activation via PI3-K/Akt during cerebral ischemia in rat hippocampus. Neurosci Lett. 2006 Aug 14;404(1-2):98-102. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	5.4374 mL	27.1872 mL	54.3744 mL	135.9361 mL
	5 mM	1.0875 mL	5.4374 mL	10.8749 mL	27.1872 mL
	10 mM	0.5437 mL	2.7187 mL	5.4374 mL	13.5936 mL
	15 mM	0.3625 mL	1.8125 mL	3.6250 mL	9.0624 mL
	20 mM	0.2719 mL	1.3594 mL	2.7187 mL	6.7968 mL
	25 mM	0.2175 mL	1.0875 mL	2.1750 mL	5.4374 mL
	30 mM	0.1812 mL	0.9062 mL	1.8125 mL	4.5312 mL
	40 mM	0.1359 mL	0.6797 mL	1.3594 mL	3.3984 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.