TMB dihydrochloride (Synonyms: BM blue dihydrochloride; Sure Blue TMB dihydrochloride)

Cat. No.: HY-15930A Purity: 99.83%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

TMB dihydrochloride (BM blue dihydrochloride) is a chromogenic substrate used in staining procedures in immunohistochemistry as well as being a visualising reagent used in enzyme-linked immunosorbent assays (ELISA).

For research use only. We do not sell to patients.

TMB dihydrochloride Chemical Structure

CAS No. : 64285-73-0

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent
10 mM * 1 mL in DMSO ready for reconstitution	USD 55	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 55	In-stock	Estimated Time of Arrival: December 31
Solid
500 mg	USD 50	In-stock	Estimated Time of Arrival: December 31
1 g	USD 60	In-stock	Estimated Time of Arrival: December 31
5 g	USD 132	In-stock	Estimated Time of Arrival: December 31
10 g		Get quote
50 g		Get quote

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This product is a controlled substance and not for sale in your territory.

Other Forms of TMB dihydrochloride:

TMB In-stock
TMB monosulfate In-stock
TMB dihydrochloride hydrate Get quote

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Biological Activity
Purity & Documentation
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Description

Molecular Weight

313.27

Formula

C₁₆H₂₂Cl₂N₂

CAS No.

64285-73-0

Appearance

Solid

Color

White to off-white

SMILES

NC1=C(C)C=C(C2=CC(C)=C(N)C(C)=C2)C=C1C.[2HCl]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMSO : ≥ 33 mg/mL (105.34 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 11.11 mg/mL (35.46 mM; Need ultrasonic)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.1921 mL	15.9607 mL	31.9213 mL
5 mM	0.6384 mL	3.1921 mL	6.3843 mL
10 mM	0.3192 mL	1.5961 mL	3.1921 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.83%

Select Batch:

Data Sheet (243 KB) SDS (0 KB)

COA (248 KB) LCMS (96 KB)

Handling Instructions (2659 KB)

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	3.1921 mL	15.9607 mL	31.9213 mL	79.8034 mL
	5 mM	0.6384 mL	3.1921 mL	6.3843 mL	15.9607 mL
	10 mM	0.3192 mL	1.5961 mL	3.1921 mL	7.9803 mL
	15 mM	0.2128 mL	1.0640 mL	2.1281 mL	5.3202 mL
	20 mM	0.1596 mL	0.7980 mL	1.5961 mL	3.9902 mL
	25 mM	0.1277 mL	0.6384 mL	1.2769 mL	3.1921 mL
	30 mM	0.1064 mL	0.5320 mL	1.0640 mL	2.6601 mL
DMSO	40 mM	0.0798 mL	0.3990 mL	0.7980 mL	1.9951 mL
	50 mM	0.0638 mL	0.3192 mL	0.6384 mL	1.5961 mL
	60 mM	0.0532 mL	0.2660 mL	0.5320 mL	1.3301 mL
	80 mM	0.0399 mL	0.1995 mL	0.3990 mL	0.9975 mL
	100 mM	0.0319 mL	0.1596 mL	0.3192 mL	0.7980 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

TMB dihydrochloride Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

TMB64285-73-0BM blueSure Blue TMBFluorescent DyeInhibitorinhibitorinhibit

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