1. Stem Cell/Wnt MAPK/ERK Pathway PI3K/Akt/mTOR Apoptosis
  2. Oct3/4 ERK Akt Apoptosis
  3. Trimebutine maleate

Trimebutine maleate is an orally anti-tumor agent. Trimebutine maleate selectively suppresses stemness and proliferation of ovarian cancer stem cells (CSCs). Trimebutine maleate reduces the colonic muscle hypercontractility, modulates gastrointestinal motility, induces apoptosis and can be used for the research of glioma/glioblastoma and irritable bowel syndrome.

For research use only. We do not sell to patients.

Trimebutine maleate Chemical Structure

Trimebutine maleate Chemical Structure

CAS No. : 34140-59-5

Size Price Stock Quantity
Free Sample (0.1 - 0.5 mg)   Apply Now  
Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
USD 55 In-stock
Solution
10 mM * 1 mL in DMSO USD 55 In-stock
Solid
1 g USD 50 In-stock
5 g USD 90 In-stock
10 g   Get quote  
50 g   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Trimebutine maleate:

Top Publications Citing Use of Products

View All ERK Isoform Specific Products:

View All Akt Isoform Specific Products:

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Trimebutine maleate is an orally anti-tumor agent. Trimebutine maleate selectively suppresses stemness and proliferation of ovarian cancer stem cells (CSCs). Trimebutine maleate reduces the colonic muscle hypercontractility, modulates gastrointestinal motility, induces apoptosis and can be used for the research of glioma/glioblastoma and irritable bowel syndrome[1][2][3][4][5][6].

In Vitro

Trimebutine maleate (1-20 μM, 60 h) dose-dependently inhibits A2780-SP cell growth [2].
Trimebutine maleate (1-10 μM, 24 h) decreases the stem cell properties of A2780-SP cells through simultaneous inhibition of the calcium and BKCa channels[2].
Trimebutine maleate (10-200 μM, 24-72 h) inhibits the cell viability, cell migration, and the ERK and AKT Signaling Pathways of glioma/glioblastoma cells [3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay[2]

Cell Line: A2780-SP cells
Concentration: 1 μM, 10 μM, 20 μM
Incubation Time: 60 h
Result: Dose-dependently inhibited A2780-SP cell proliferation.

Cell Migration Assay [3]

Cell Line: U-87 MG human glioblastoma cells
Concentration: 10 μM, 50 μM, 100 μM, 200 μM
Incubation Time: 24 h, 48 h, 72 h
Result: Significantly impeded gap closure, with the most effective inhibition observed at 200 µM for all time points after 24, 48, and 72 h of incubation.

Apoptosis Analysis[2]

Cell Line: A2780-SP cells
Concentration: 1 μM, 10 μM
Incubation Time: 24 h
Result: Induced G0/G1 arrest and significantly increased the population of AV+/PI+ cells at concentrations over 1 µM.

Western Blot Analysis[2]

Cell Line: A2780-SP cells
Concentration: 1 μM, 10 μM
Incubation Time: 24 h
Result: Especially reduced OCT3/4 and SOX2 protein expression related to stemness and ERK phosphorylation related to cell growth, respectively.
In Vivo

Trimebutine maleate (25-50 mg/kg Oral, single dose) reduces the response to colorectal distension in C57Bl6 male mice[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial
Molecular Weight

503.54

Formula

C26H33NO9

CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(O)/C=C\C(O)=O.O=C(OCC(C1=CC=CC=C1)(N(C)C)CC)C2=CC(OC)=C(OC)C(OC)=C2

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (198.59 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 25 mg/mL (49.65 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.9859 mL 9.9297 mL 19.8594 mL
5 mM 0.3972 mL 1.9859 mL 3.9719 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (4.96 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (4.96 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 1.9859 mL 9.9297 mL 19.8594 mL 49.6485 mL
5 mM 0.3972 mL 1.9859 mL 3.9719 mL 9.9297 mL
10 mM 0.1986 mL 0.9930 mL 1.9859 mL 4.9648 mL
15 mM 0.1324 mL 0.6620 mL 1.3240 mL 3.3099 mL
20 mM 0.0993 mL 0.4965 mL 0.9930 mL 2.4824 mL
25 mM 0.0794 mL 0.3972 mL 0.7944 mL 1.9859 mL
30 mM 0.0662 mL 0.3310 mL 0.6620 mL 1.6549 mL
40 mM 0.0496 mL 0.2482 mL 0.4965 mL 1.2412 mL
DMSO 50 mM 0.0397 mL 0.1986 mL 0.3972 mL 0.9930 mL
60 mM 0.0331 mL 0.1655 mL 0.3310 mL 0.8275 mL
80 mM 0.0248 mL 0.1241 mL 0.2482 mL 0.6206 mL
100 mM 0.0199 mL 0.0993 mL 0.1986 mL 0.4965 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

Trimebutine maleate Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

 

Salutation

Applicant Name *

 

Email Address *

Phone Number *

 

Organization Name *

Department *

 

Requested quantity *

Country or Region *

     

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
Trimebutine maleate
Cat. No.:
HY-B0380A
Quantity:
MCE Japan Authorized Agent: