2. Immunology/Inflammation
  3. COX
  4. (-)-Catechin gallate

(-)-Catechin gallate  (Synonyms: (-)-Catechin 3-gallate; (-)-Catechin 3-O-gallate)

Cat. No.: HY-N0356 Purity: 99.74%
COA Handling Instructions

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(-)-Catechin gallate is a minor constituent in green tea catechins. (-)-Catechin gallate inhibits the activity of COX-1 and COX-2 enzymes.

For research use only. We do not sell to patients.

(-)-Catechin gallate Chemical Structure

(-)-Catechin gallate Chemical Structure

CAS No. : 130405-40-2

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 336 In-stock
Solution
10 mM * 1 mL in DMSO USD 336 In-stock
Solid
1 mg USD 139 In-stock
5 mg USD 306 In-stock
10 mg USD 492 In-stock
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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of (-)-Catechin gallate:

(-)-Catechin gallate Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE (-)-Catechin gallate

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  • Biological Activity

  • Protocol

  • Purity & Documentation

  • References

  • Customer Review

Description

(-)-Catechin gallate is a minor constituent in green tea catechins. (-)-Catechin gallate inhibits the activity of COX-1 and COX-2 enzymes.

IC50 & Target

COX-1

 

COX-2

 

In Vitro

(-)-Catechin gallate (CG) directly interacts with DNA oligomers and inhibits the activity of COX-1 and COX-2 enzymes, the gene expression of matrix metalloproteinase-9 in macrophage-differentiated HL-60 myeloid leukemia cells, the adipocyte uptake of glucose by the transporter, GLUT4, and the activities of various proteasomes, i.e., the multicatalytic proteases responsible for the degradation of most cellular proteins. The relative cytotoxicities of a 3-day exposure to (-)-Catechin gallate are determined for cancerous CAL27 and HSG cells, immortalized epithelioid S-G cells, and normal HGF-1 gingival fibroblasts. The concentration at which toxicity (P≤0.01) initially occur is 25 μM (-)-Catechin gallate for S-G cells, 50 μM (-)-Catechin gallate for CAL27 cells, 62.5 μM (-)-Catechin gallate for HSG cells and 75 μM (-)-Catechin gallate for HGF-1 fibroblasts. The calculated neutral red (NR50) values for a 3-day exposure to (-)-Catechin gallate are 58 μM for S-G cells, 62 μM for CAL27 cells, 90 μM for HSG cells and 132 μM for HGF-1 fibroblasts[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

(-)-Catechin gallate Related Antibodies
Molecular Weight

442.37

Formula

C22H18O10
CAS No.
Appearance

Solid

SMILES

O=C(O[C@H]1[C@H](C2=CC=C(O)C(O)=C2)OC3=CC(O)=CC(O)=C3C1)C4=CC(O)=C(O)C(O)=C4
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (226.06 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.2606 mL 11.3028 mL 22.6055 mL
5 mM 0.4521 mL 2.2606 mL 4.5211 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (5.65 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (5.65 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.98%

COA (255 KB) HNMR (306 KB)

Handling Instructions (2659 KB)
References
Cell Assay
[1]

Human tongue squamous carcinoma (CAL27) cells and human salivary gland carcinoma (HSG) cells are used. Individual wells of a 96-well microtiter tissue culture plate are inoculated with 0.2 mL of the growth medium containing 2×104 cells/well for a 1-day exposure, 1.5×104 cells/ well for a 2-day exposure and 1×104 cells/well for a 3-day exposure to the test agents. After 1 day of incubation, the growth medium is removed and replaced with exposure medium, with or without varied concentrations of the test agents. In some studies the cells are coexposed to (-)-Catechin gallate (100, 200, 300, 400, and 500 μM) and 100 Units/mL catalase. After 1-3 days of exposure to the test agents, viability is assessed with the neutral red (NR) assay, which is based on the uptake and accumulation of the supravital dye, neutral red (NR)[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.2606 mL 11.3028 mL 22.6055 mL 56.5138 mL
5 mM 0.4521 mL 2.2606 mL 4.5211 mL 11.3028 mL
10 mM 0.2261 mL 1.1303 mL 2.2606 mL 5.6514 mL
15 mM 0.1507 mL 0.7535 mL 1.5070 mL 3.7676 mL
20 mM 0.1130 mL 0.5651 mL 1.1303 mL 2.8257 mL
25 mM 0.0904 mL 0.4521 mL 0.9042 mL 2.2606 mL
30 mM 0.0754 mL 0.3768 mL 0.7535 mL 1.8838 mL
40 mM 0.0565 mL 0.2826 mL 0.5651 mL 1.4128 mL
50 mM 0.0452 mL 0.2261 mL 0.4521 mL 1.1303 mL
60 mM 0.0377 mL 0.1884 mL 0.3768 mL 0.9419 mL
80 mM 0.0283 mL 0.1413 mL 0.2826 mL 0.7064 mL
100 mM 0.0226 mL 0.1130 mL 0.2261 mL 0.5651 mL
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(-)-Catechin gallate Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

(-)-Catechin gallate130405-40-2(-)-Catechin 3-gallate (-)-Catechin 3-O-gallateCOXCyclooxygenaseInhibitorinhibitorinhibit

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