2. C2 Ceramide

C2 Ceramide  (Synonyms: Ceramide 2)

Cat. No.: HY-101180 Purity: 99.85%
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C2 Ceramide (Ceramide 2) is the main lipid of the stratum corneum and a protein phosphatase 1 (PP1) activator. C2 Ceramide activates PP2A and ceramide-activated protein phosphatase (CAPP). C2 Ceramide induces cells differentiation, autophagy and apoptosis, inhibits mitochondrial respiratory chain complex III. C2 Ceramide is also a skin conditioning agent that protects the epidermal barrier from water loss.

For research use only. We do not sell to patients.

C2 Ceramide Chemical Structure

C2 Ceramide Chemical Structure

CAS No. : 3102-57-6

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1 mg USD 35 In-stock
5 mg USD 125 In-stock
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Based on 1 publication(s) in Google Scholar

C2 Ceramide Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE C2 Ceramide

  • Biological Activity

  • Purity & Documentation

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Description

C2 Ceramide (Ceramide 2) is the main lipid of the stratum corneum and a protein phosphatase 1 (PP1) activator. C2 Ceramide activates PP2A and ceramide-activated protein phosphatase (CAPP). C2 Ceramide induces cells differentiation, autophagy and apoptosis, inhibits mitochondrial respiratory chain complex III. C2 Ceramide is also a skin conditioning agent that protects the epidermal barrier from water loss[1][2][3][4][5].

IC50 & Target

Protein phosphatase 1[2]
PP2A[4]
Ceramide-activated protein phosphatase (CAPP)[4]
Apoptosis[1]
Mitochondrial respiratory chain complex III[1]
In Vitro

C2 Ceramide (5 nM-200 µM; 24 hours; primary mouse osteoblasts) treatment (≤500 nM) promots osteoblast viability, whilst concentrations ≥2 µM significantly reduces osteoblast viability in a dose- and time-dependent manner[1].
C2 Ceramide increases cytoplasmic histone-associated DNA fragments by 5.7- and 11.2-fold at 50 µM and 100 µM C2 Ceramide concentrations respectively in osteoblasts. At these higher concentrations, C2 Ceramide is a potent inducer of apoptosis in osteoblasts[1].
C2 Ceramide up-regulates mRNA expression of angiogenic genes in human dental pulp cells (HDPCs) and increases the migration and capillary tube formation of endothelial cells, whereas PP1 small interfering RNA shows opposite effects. Human dental pulp cells (HDPCs) increases levels of bone morphogenetic protein 2, phosphorylation of Smad 1/5/8, and mRNA expression of runt-related transcription factor 2 and osterix[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

C2 Ceramide Related Antibodies

Cell Viability Assay[1]

Cell Line: Primary mouse osteoblasts
Concentration: 5 nM-200 µM
Incubation Time: 24 hours
Result: Murine osteoblasts demonstrated a dose-dependent increase in their survival rate when exposed to low concentrations of 5-500 n M. Increasing concentrations of 20-200µM caused a dose-dependent decrease in mitochondrial succinate dehydrogenase activity and osteoblast survival.
In Vivo

The PP1 activator C2 Ceramide increases alkaline phosphatase activity, mineralizes nodule formation, and mRNA expression of dentin matrix protein 1 and dentin sialophosphoprotein. In contrast, knockdown by PP1 small interfering RNA inhibits odontoblastic differentiation[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

341.53

Formula

C20H39NO3
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O[C@H](/C=C/CCCCCCCCCCCCC)[C@H](CO)NC(C)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 20 mg/mL (58.56 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Ethanol : 17 mg/mL (49.78 mM; Need ultrasonic and warming)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.9280 mL 14.6400 mL 29.2800 mL
5 mM 0.5856 mL 2.9280 mL 5.8560 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.85%

COA (240 KB) HNMR (279 KB) RP-HPLC (241 KB) MS (70 KB)

Handling Instructions (2659 KB)

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
Ethanol / DMSO 1 mM 2.9280 mL 14.6400 mL 29.2800 mL 73.2000 mL
5 mM 0.5856 mL 2.9280 mL 5.8560 mL 14.6400 mL
10 mM 0.2928 mL 1.4640 mL 2.9280 mL 7.3200 mL
15 mM 0.1952 mL 0.9760 mL 1.9520 mL 4.8800 mL
20 mM 0.1464 mL 0.7320 mL 1.4640 mL 3.6600 mL
25 mM 0.1171 mL 0.5856 mL 1.1712 mL 2.9280 mL
30 mM 0.0976 mL 0.4880 mL 0.9760 mL 2.4400 mL
40 mM 0.0732 mL 0.3660 mL 0.7320 mL 1.8300 mL
DMSO 50 mM 0.0586 mL 0.2928 mL 0.5856 mL 1.4640 mL
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

C2 Ceramide3102-57-6Ceramide 2Ceramide2Ceramide-2Inhibitorinhibitorinhibit

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