2. Cell Cycle/DNA Damage Cytoskeleton
  3. Microtubule/Tubulin
  4. CHM-1

CHM-1, a microtubule-destabilizing agent, inhibits tubulin polymerization. CHM-1 is a potent and selective antimitotic antitumor activity against human hepatocellular carcinoma. CHM-1 induces growth inhibition and apoptosis via G2-M phase arrest in human hepatocellular carcinoma cells by activation of Cdc2 kinase activity.

For research use only. We do not sell to patients.

CHM-1 Chemical Structure

CHM-1 Chemical Structure

CAS No. : 154554-41-3

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Based on 1 publication(s) in Google Scholar

CHM-1 Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

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Description

CHM-1, a microtubule-destabilizing agent, inhibits tubulin polymerization. CHM-1 is a potent and selective antimitotic antitumor activity against human hepatocellular carcinoma. CHM-1 induces growth inhibition and apoptosis via G2-M phase arrest in human hepatocellular carcinoma cells by activation of Cdc2 kinase activity[1][2][3].

IC50 & Target

IC50: 0.75 μM (HA22T)[1]
In Vitro

CHM-1 (0-100μM; 24 hours) induces significant concentration-dependent growth inhibition in HA22T, Hep3B, and HepG2 cells, with the most potent effects observed in HA22T cells (IC50 = 0.75 μM)[1].
CHM-1 (0-10 μM; 24 hours) significantly increases the binding of cyclin B1 to Cdc2 in HA22T cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

CHM-1 Related Antibodies

Cell Viability Assay[1]

Cell Line: HA22T, Hep3B, and HepG2 cells
Concentration: 0-100 μM
Incubation Time: 24 hours
Result: Induced G2-M arrest of the cell cycle followed by apoptosis.

Western Blot Analysis[1]

Cell Line: HA22T cells
Concentration: 0-10 μM
Incubation Time: 24 hours
Result: Induced change in expressed and phosphorylated status of G2-M regulators in human hepatocellular carcinoma cells.
In Vivo

CHM-1 (10 mg/kg; I.p.) induces a dose-dependent inhibition of HA22T tumor growth[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male severe combined immunodeficient mice (HA22T)[1]
Dosage: 10 mg/kg
Administration: I.p.
Result: Induced a dose-dependent inhibition of HA22T tumor growth.
Molecular Weight

283.25

Formula

C16H10FNO3
CAS No.
Appearance

Solid

Color

Off-white to light yellow

SMILES

O=C1C=C(C2=CC=CC=C2F)NC3=C1C=C4C(OCO4)=C3
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 5 mg/mL (17.65 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.5305 mL 17.6523 mL 35.3045 mL
5 mM 0.7061 mL 3.5305 mL 7.0609 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.57%

COA (251 KB) HNMR (250 KB) LCMS (93 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.5305 mL 17.6523 mL 35.3045 mL 88.2613 mL
5 mM 0.7061 mL 3.5305 mL 7.0609 mL 17.6523 mL
10 mM 0.3530 mL 1.7652 mL 3.5305 mL 8.8261 mL
15 mM 0.2354 mL 1.1768 mL 2.3536 mL 5.8841 mL
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CHM-1 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

CHM-1154554-41-3NSC656158CHM1CHM 1NSC 656158NSC-656158Microtubule/Tubulinmicrotubule-destabilizingantimitoticantitumorhepatocellularcarcinomaCdc2Inhibitorinhibitorinhibit

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