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  4. EDTA-AM

EDTA-AM  (Synonyms: EDTA acetoxymethyl ester; Ethylenediaminetetraacetic acid acetoxymethyl ester)

Cat. No.: HY-D1746 Purity: ≥98.0%
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EDTA-AM (ethylenediaminetetraacetic acid, acetoxymethyl ester) is the membrane-permeant form of the metal chelator EDTA (HY-Y0682). Live cells passively load EDTA-AM by incubating with EDTA-AM. Once internalized, cytoplasmic esterase decomposes AM esters, releasing the active ligand EDTA, which isolates metal ions within the cell. EDTA-AM induces an arrest of mitotic progression and chromosome decondensation.

For research use only. We do not sell to patients.

EDTA-AM Chemical Structure

EDTA-AM Chemical Structure

CAS No. : 162303-59-5

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5 mg USD 125 In-stock
10 mg USD 200 In-stock
25 mg USD 400 In-stock
50 mg USD 640 In-stock
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Based on 1 publication(s) in Google Scholar

EDTA-AM Related Antibodies

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Description

EDTA-AM (ethylenediaminetetraacetic acid, acetoxymethyl ester) is the membrane-permeant form of the metal chelator EDTA (HY-Y0682). Live cells passively load EDTA-AM by incubating with EDTA-AM. Once internalized, cytoplasmic esterase decomposes AM esters, releasing the active ligand EDTA, which isolates metal ions within the cell. EDTA-AM induces an arrest of mitotic progression and chromosome decondensation[1][2].

In Vitro

EDTA-AM treatment of mitotic cells：
1. Mitotic cells were collected and seeded onto a 12-well culture plate with poly lysine-coated coverslips and DMEM supplemented with 10% FBS.
2. EDTA-AM was dissolved in 100 mM DMSO, EDTA-AM was added to the culture medium at a final concentration of 20 mM with 0.02% Pluronic F-127 (Invitrogen), and the cells were further incubated at 37C for 100 min.
3.After incubation, the numbers of mitotic cells and interphase cells were counted. The cells on the coverslips were fixed, stained with DAPI, and mounted with para-phenylene diamine (PPDI) solution (20 mM HEPES, pH 7.4, 1 mM MgCl2, 100 mM KCl, 78% glycerol, 1 mg/mL PPDI).

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

EDTA-AM Related Antibodies
Molecular Weight

580.49

Formula

C22H32N2O16
CAS No.
Appearance

Liquid

Color

Colorless to light yellow

SMILES

O=C(C)OCOC(CN(CCN(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (172.27 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.7227 mL 8.6134 mL 17.2268 mL
5 mM 0.3445 mL 1.7227 mL 3.4454 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Concentration
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Volume
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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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C2

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V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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(per animal)

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.7227 mL 8.6134 mL 17.2268 mL 43.0671 mL
5 mM 0.3445 mL 1.7227 mL 3.4454 mL 8.6134 mL
10 mM 0.1723 mL 0.8613 mL 1.7227 mL 4.3067 mL
15 mM 0.1148 mL 0.5742 mL 1.1485 mL 2.8711 mL
20 mM 0.0861 mL 0.4307 mL 0.8613 mL 2.1534 mL
25 mM 0.0689 mL 0.3445 mL 0.6891 mL 1.7227 mL
30 mM 0.0574 mL 0.2871 mL 0.5742 mL 1.4356 mL
40 mM 0.0431 mL 0.2153 mL 0.4307 mL 1.0767 mL
50 mM 0.0345 mL 0.1723 mL 0.3445 mL 0.8613 mL
60 mM 0.0287 mL 0.1436 mL 0.2871 mL 0.7178 mL
80 mM 0.0215 mL 0.1077 mL 0.2153 mL 0.5383 mL
100 mM 0.0172 mL 0.0861 mL 0.1723 mL 0.4307 mL
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EDTA-AM Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

EDTA-AM162303-59-5EDTA acetoxymethyl ester Ethylenediaminetetraacetic acid acetoxymethyl esterBiochemical Assay ReagentsEDTAmetal chelatormitosischromosome decondensationInhibitorinhibitorinhibit

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