2. Ethyl pyruvate

Ethyl pyruvate is a simple derivative of the endogenous metabolite pyruvate. Ethyl pyruvate is an HMGB1 release inhibitor. Ethyl pyruvate can induce apoptosis by autophagy. Ethyl pyruvate has anti-inflammatory, antioxidant and anti-tumor activity. Ethyl pyruvate can be used in the study of neurodegenerative diseases such as Alzheimer's and Parkinson's disease.

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Ethyl pyruvate Chemical Structure

Ethyl pyruvate Chemical Structure

CAS No. : 617-35-6

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Based on 5 publication(s) in Google Scholar

Other Forms of Ethyl pyruvate:

Ethyl pyruvate Related Antibodies

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Description

Ethyl pyruvate is a simple derivative of the endogenous metabolite pyruvate. Ethyl pyruvate is an HMGB1 release inhibitor. Ethyl pyruvate can induce apoptosis by autophagy. Ethyl pyruvate has anti-inflammatory, antioxidant and anti-tumor activity. Ethyl pyruvate can be used in the study of neurodegenerative diseases such as Alzheimer's and Parkinson's disease[1][2][3][4][5].

In Vitro

Ethyl pyruvate (10 mM, 1 h) has no toxic effect on N9 microglial cells in the range of 1-10 mM. The activation of microglia NLRP3 inflammasome is decreased by inhibiting the HMGB1/ NF-κB /miR-223 signaling pathway[2].
Ethyl pyruvate (10-40 mM, 6, 24 h) induces apoptosis in MC38 cells[3].
Ethyl pyruvate (5-15 mM, 2 h) has an IC50 value of 28.83 mM on mouse peritoneal macrophages. Endotoxemia and sepsis are prevented by inhibiting caspase-11-dependent pyroptosis [4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Ethyl pyruvate Related Antibodies

Cell Viability Assay[2]

Cell Line: N9 microglial
Concentration: 1-100 mM
Incubation Time: 1 h
Result: Did not show cytotoxic effects in the range of 1–10 mM.

Western Blot Analysis[2]

Cell Line: N9 microglial
Concentration: 10 mM
Incubation Time: 1 h
Result: Suppressed LPS (HY-D1056)- and ATP (HY-B2176)-induced IL-1β and IL-18 protein and mRNA levels.
Reduced NLRP3, Caspase-1, and ASC Specks.
Reduced NF-κB activation and HMGB1 expression level.

Apoptosis Analysis[3]

Cell Line: MC38
Concentration: 10, 20, 40 mM
Incubation Time: 6, 24 h
Result: Induced an increase in autophagy and apoptosis in a dose-and time-dependent manner.
In Vivo

Ethyl pyruvate (80 mg/kg intraperitoneal injection for 9 consecutive days) inhibits tumor growth in a mouse liver tumor model[3].
Ethyl pyruvate (2 or 40 mg/kg, intraperitoneal injection) has reduced lipid peroxidation and anti-inflammatory effects in a rat model of paraquat intoxication[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Liver tumor model in mice[3]
Dosage: 80 mg/kg
Administration: i.p., 30 min before tumor injection and daily up to 9 days and daily from 7 to 10 days after infusion of tumor cells.
Result: Decreased innate immune cells (NK cells, monocytes) and T and B cell lymphocytic infiltrates.
Inhibited the release of HMBG1.
Animal Model: Paraquat-intoxicated rats[5]
Dosage: 2 or 40 mg/kg
Administration: 30 min before or 1 h after paraquat (50 mg/kg i.p.)
Result: Decreased the MDA concentrations at 6 and 24 h.
Decreased NO concentrations significantly at 6 h and GSH concentrations in the lung.
Clinical Trial
Molecular Weight

116.12

Formula

C5H8O3
CAS No.
Appearance

Liquid

Color

Colorless to light yellow

SMILES

CC(C(OCC)=O)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Pure form -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

H2O : 100 mg/mL (861.18 mM; Need ultrasonic)

DMSO : 100 mg/mL (861.18 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 8.6118 mL 43.0589 mL 86.1178 mL
5 mM 1.7224 mL 8.6118 mL 17.2236 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 99.87%

COA (246 KB) HNMR (115 KB) GC (203 KB)

Handling Instructions (2659 KB)

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 8.6118 mL 43.0589 mL 86.1178 mL 215.2945 mL
5 mM 1.7224 mL 8.6118 mL 17.2236 mL 43.0589 mL
10 mM 0.8612 mL 4.3059 mL 8.6118 mL 21.5295 mL
15 mM 0.5741 mL 2.8706 mL 5.7412 mL 14.3530 mL
20 mM 0.4306 mL 2.1529 mL 4.3059 mL 10.7647 mL
25 mM 0.3445 mL 1.7224 mL 3.4447 mL 8.6118 mL
30 mM 0.2871 mL 1.4353 mL 2.8706 mL 7.1765 mL
40 mM 0.2153 mL 1.0765 mL 2.1529 mL 5.3824 mL
50 mM 0.1722 mL 0.8612 mL 1.7224 mL 4.3059 mL
60 mM 0.1435 mL 0.7176 mL 1.4353 mL 3.5882 mL
80 mM 0.1076 mL 0.5382 mL 1.0765 mL 2.6912 mL
100 mM 0.0861 mL 0.4306 mL 0.8612 mL 2.1529 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Ethyl pyruvate617-35-6Inhibitorinhibitorinhibit

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