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  6. Indomethacin sodium hydrate

Indomethacin sodium hydrate  (Synonyms: Indometacin sodium hydrate)

Cat. No.: HY-14397A Purity: 99.80%
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Indomethacin (Indometacin) sodium hydrateis a potent, orally active COX1/2 inhibitor with IC50 values of 18 nM and 26 nM for COX-1 and COX-2, respectively. Indomethacin sodium hydrateis has anticancer activity and anti-infective activity. Indomethacin sodium hydrateis can be used for cancer, inflammation and viral infection research.

For research use only. We do not sell to patients.

Indomethacin sodium hydrate Chemical Structure

Indomethacin sodium hydrate Chemical Structure

CAS No. : 74252-25-8

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10 mM * 1 mL in DMSO
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Based on 31 publication(s) in Google Scholar

Other Forms of Indomethacin sodium hydrate:

Indomethacin sodium hydrate Related Antibodies

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31 Publications Citing Use of MCE Indomethacin sodium hydrate

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  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Indomethacin (Indometacin) sodium hydrateis a potent, orally active COX1/2 inhibitor with IC50 values of 18 nM and 26 nM for COX-1 and COX-2, respectively. Indomethacin sodium hydrateis has anticancer activity and anti-infective activity. Indomethacin sodium hydrateis can be used for cancer, inflammation and viral infection research[1][2][3].

In Vitro

Indomethacin (Indometacin) sodium hydrateis (0-150 μM; 24 hours; 3LL-D122 cells) has anticancer activity in vitro[2].
? Indomethacin (Indometacin) sodium hydrateis (0-1000 μM) protects the host cells from damage caused by the virus through activates PKR, resulting in elF2α phosphorylation, and in turn shutting of translation of viral protein and inhibiting replication of the virus (IC50=2 μM)[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Indomethacin sodium hydrate Related Antibodies

Cell Viability Assay[2]

Cell Line: 3LL-D122 cells (highly metastatic variant of mouse LLcarcinoma cells)
Concentration: 0, 20, 50, 100 and 150μM
Incubation Time: 24 hours
Result: Inhibited cell viability at 20 mM, with 50% inhibition at 60 mM.

Cell Cycle Analysis[2]

Cell Line: 3LL-D122 cells (highly metastatic variant of mouse LLcarcinoma cells)
Concentration: 0, 30 and 80μM
Incubation Time: 24 hours
Result: Decreased in the percentage of cells at the G2/M phase and increased in the percentage of cells at G1 phase.
In Vivo

Indomethacin (Indometacin) sodium hydrateis (0.01-10 mg/kg; p.o.; for 3 hours; male Sprague-Dawley rats) induces paw oedema and hyperalgesmeasurement dose-dependently reversed carrageenan-induced hyperalgesia[1].
? Indomethacin (Indometacin) sodium hydrateis (10 mg/mL; p.o.; daily, for 29 days; male C57BL/6J mice) inhibits tumor growth in vivo[2].Following oral administration of Indomethacin, the absorption of the drug is rapid and complete, but with important inter-and intraindividual variations. In general, peak plasma concentrations of 2 to 3 microgram/ml are achieved with 1 to 2 hours, but concomitant ingestion of food reduces and delays the peak concentrations without reducing the amount absorbed. In plasma at 90% of indomethacin is bound to albumin at therapeutic plasma concentrations[4].
Indomethacin can be used in animal modeling to construct models of gastric ulceration.
Induction of gastric ulceration[5][6]
Background
Indomethacin can cause gastric ulceration by various mechanisms, including injury through inhibition of prostaglandin (PG) synthesis, reduction in local blood flow, regional irritation, and inhibition of tissue regeneration.
Specific Mmodeling Methods
Rats: albino Sprague-Dawley • male • adult (period: 2 weeks)
Administration: 100 mg/kg • p.o. • single dose
Note
(1) All animals fasted 24 h before drug administration.
(2) Indomethacin were dissolved in saline with 5% NaOH.


Modeling Indicators
Gastric tissue macroscopic alterations: Showed prominent mucosal folds and severe erosion, pronounced ulceration and bleeding foci in the gastric mucosa.
Histopathological changes: Showed severe erosion of the mucosa, reaching down to the lamina muscularis; observed hemorrhagic infiltration, edema in the submucosa, and severe hyperemia of the vessels.
Molecular changes: Showed intense Tnf-α expression.
Biochemical changes: Increased MDA, TOS levels, reduced TAS levels, CAT and GPx activities and GSH levels.
Correlated Product(s): Indomethacin sodium hydrate (HY-14397A)
Opposite Product(s): Carnosic acid (HY-N0644)

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male Sprague-Dawley rats[1]
Dosage: 0.01-10 mg/kg
Administration: Oral administration; for 3 hours
Result: Inhibited the carrageenan-induced rat paw oedema (ED50=2.0 mg/kg) and hyperalgesia (ED50=1.5 mg/kg) in a dose-dependent manner.
Animal Model: Male C57BL/6J mice[2]
Dosage: 10 mg/mL
Administration: Oral administration; daily, for 29 days
Result: Delayed the onset of tumor growth and the initial growth rate of the footpad tumors.
Clinical Trial
Molecular Weight

433.82

Formula

C19H21ClNNaO7
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C(O[Na])CC1=C(C)N(C(C2=CC=C(Cl)C=C2)=O)C3=C1C=C(OC)C=C3.O.O.O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility
In Vitro: 

H2O : 25 mg/mL (57.63 mM; Need ultrasonic)

DMSO : 12.5 mg/mL (28.81 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.3051 mL 11.5255 mL 23.0510 mL
5 mM 0.4610 mL 2.3051 mL 4.6102 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

=
Concentration (final)

C2

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Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (4.79 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.08 mg/mL (4.79 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 5 mg/mL (11.53 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 99.80%

COA (255 KB) HNMR (275 KB) LCMS (263 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO / H2O 1 mM 2.3051 mL 11.5255 mL 23.0510 mL 57.6276 mL
5 mM 0.4610 mL 2.3051 mL 4.6102 mL 11.5255 mL
10 mM 0.2305 mL 1.1526 mL 2.3051 mL 5.7628 mL
15 mM 0.1537 mL 0.7684 mL 1.5367 mL 3.8418 mL
20 mM 0.1153 mL 0.5763 mL 1.1526 mL 2.8814 mL
25 mM 0.0922 mL 0.4610 mL 0.9220 mL 2.3051 mL
H2O 30 mM 0.0768 mL 0.3842 mL 0.7684 mL 1.9209 mL
40 mM 0.0576 mL 0.2881 mL 0.5763 mL 1.4407 mL
50 mM 0.0461 mL 0.2305 mL 0.4610 mL 1.1526 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Indomethacin sodium74252-25-8Indometacin sodiumCOXBacterialInfluenza VirusAntibioticCyclooxygenaseanti-infectiveanticancerinflammationCOX-1COX-2Inhibitorinhibitorinhibit

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