1. MCE Kits
  2. Molecular Biology
  3. Restriction Endonuclease
  4. BsmB I

BsmB I 

Cat. No.: HY-KE7050
Manual SDS

BsmB I is one of the endonucleases of Type IIs that recognize non-palindromic sequences and cut outside of the recognition sequence, and is commonly used in Golden Gate assembly. Isoschizomers: Esp3 I, BstGZ53 I, Esp16 I, Esp23 I.

BsmB I
Size Price Stock Quantity
1000U USD 456 In-stock

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  • Description

  • Storage

  • Components

  • Documentation

Description
& Advantages

•    BsmB I is one of the endonucleases of Type IIs that recognize non-palindromic sequences and cut outside of the recognition sequence, and is commonly used in Golden Gate assembly. BsmB I cuts best at 55℃. To ensure consistent performance, this product contains premixed BSA, which enhances the stability of BsmB I and binds contaminants that may be present in DNA preparations. Isoschizomers: Esp3 I, BstGZ53 I, Esp16 I, Esp23 I.

 

•   Cleavage site:

 

5’…C G T C T C (N)1…3’


3’…G C A G A G (N)5…5’
Storage
-20°C, 2 years.
Components
Components HY-KE7050-1000U
BsmB I (10 U/μL) 100 μL
10× BsmB I Buffer 1 mL
Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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