Basement Membrane Matrix for Organoid Culture 

-20°C, 2 years.

Avoid repeated freezing and thawing.

Thin Gel Method. For cell migration and invasion, primary cell expansion: Dilute with pre-cooled medium according to experimental needs after thawing. It is recommended that the concentration be no less than 1 mg/mL. Add 50 μL/cm² of matrix to the surface of the cell culture plate and spread it evenly, taking care to avoid air bubbles. Place the plate at 37 ℃ and incubate for 0.5h, aspirate the supernatant to use.

Thin Coating Method. For primary cell expansion: Dilute with pre-cooled medium according to experimental needs after thawing. It is recommended that the concentration be no less than 0.1 mg/mL. Aspirate the appropriate amount of dilution to completely cover the surface of the cell culture plate, the recommended amount of coating is 0.01-0.02 mg/cm². Place the plate at 37 ℃ and incubate for 1h, aspirate the supernatant to use.

Gel embedding Method. For organoid culture and tumor spheroid invasion: Thaw the product in advance and set aside. Prepare the desired cells and resuspend them with matrix, it is recommended that the proportion of matrix is >70%. Add 15-20 μL/cm² to the surface of the cell culture plate, taking care to avoid air bubbles. Place the plate at 37 ℃ for 0.5h to form a gel that wraps the cells, and add the appropriate medium to the plate.