2. Academic Validation
  3. Phenolic constituents isolated from Fragaria ananassa Duch. inhibit antigen-stimulated degranulation through direct inhibition of spleen tyrosine kinase activation

Phenolic constituents isolated from Fragaria ananassa Duch. inhibit antigen-stimulated degranulation through direct inhibition of spleen tyrosine kinase activation

  • Bioorg Med Chem. 2010 Aug 15;18(16):5932-7. doi: 10.1016/j.bmc.2010.06.083.
Masayuki Ninomiya 1,  Tomohiro Itoh,  Suguru Ishikawa,  Miho Saiki,  Kenji Narumiya,  Masaharu Yasuda,  Kaneyuki Koshikawa,  Yoshinori Nozawa,  Mamoru Koketsu
Affiliations

Affiliation

  • 1 Department of Materials Science and Technology, Faculty of Engineering, Gifu University, Gifu, Japan.
PMID: 20663674 DOI: 10.1016/j.bmc.2010.06.083
Abstract

We isolated eight phenolic constituents from Fragaria ananassa Duch. (strawberry) and determined their structures using 1D, 2D-NMR. Among the isolated compounds, linocinnamarin (LN), 1-O-trans-cinnamoyl-beta-d-glucopyranose (CG), and cinnamic acid (CA) exhibited antigen (Ag)-stimulated degranulation in rat basophilic leukemia RBL-2H3 cells. In order to reveal the underlying mechanisms, we examined the effects of LN and CA on cellular responses induced by antigen stimulation. Treatment with both LN and CA markedly inhibited antigen-stimulated elevation of intracellular free Ca(2+) concentration and Reactive Oxygen Species (ROS). Both LN and CA suppressed Ag-stimulated spleen tyrosine kinase (Syk) activation. These results indicate that inhibition of antigen-stimulated degranulation by LN and CA is mainly due to inactivation of Syk/Phospholipase Cgamma (PLCgamma) pathways. Our findings suggest that LN and CA isolated from F. ananassa Duch. (strawberry) could be beneficial agents for alleviating symptoms of type I allergy.

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