1. Academic Validation
  2. Angiotensin II type I receptor and miR-155 in endometrial cancers: synergistic antiproliferative effects of anti-miR-155 and losartan on endometrial cancer cells

Angiotensin II type I receptor and miR-155 in endometrial cancers: synergistic antiproliferative effects of anti-miR-155 and losartan on endometrial cancer cells

  • Gynecol Oncol. 2012 Jul;126(1):124-31. doi: 10.1016/j.ygyno.2012.04.020.
Chel Hun Choi 1 Young-Ae Park Jung-Joo Choi Taejong Song Sang Yong Song Yoo-Young Lee Jeong-Won Lee Tae-Joong Kim Byoung-Gie Kim Duk-Soo Bae
Affiliations

Affiliation

  • 1 Department of Obstetrics and Gynecology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Republic of Korea.
Abstract

Objective: MicroRNA-155 (miR-155) is one of the micro RNAs (miRNA) most consistently involved in neoplastic diseases, and it is known to repress the angiotensin II type 1 receptor (AGTR1). The aim of the present study was to evaluate the expressions of miR-155 and AGTR1, and to clarify the potential efficacy of anti-miR-155, alone and in combination with AGTR1 blocker losartan in endometrial cancers.

Methods: Expressions of miR-155 and AGTR1 were evaluated using Real-Time PCR and immunohistochemistry. And the MTT assay was performed in endometrial Cancer cells following anti-miR-155 and AGTR1 blocker (losartan) treatment, alone and in combination.

Results: miR-155 was over-expressed and AGTR1 was underexpressed in endometrial carcinoma tissues. AGTR1 immunoreactivity was found in six of ten (60.0%) normal endometrium, 11 of 14 (78.6%) endometrial hyperplasia, and 27 of 62 (43.5%) endometrial carcinoma tissues (P=0.051), and patients with AGTR1 expression showed trend towards improved survival after multivariate analysis (P=0.08). We checked that abolishing the function of miR-155 and AGTR1 by anti-miR-155 or losartan inhibited cell survival of endometrial carcinoma cells, respectively, and furthermore, combined treatment showed synergistic effects.

Conclusions: In this study, we characterized the expressions of miR-155 and AGTR1 in endometrial tissues. The combined treatment with anti-miR-155 and losartan has a synergistic antiproliferative effect and an improved understanding is required to clarify whether miR-155 and AGTR1 can be used as a novel therapeutic target in endometrial Cancer.

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