2. Academic Validation
  3. Construction of a convenient system for easily screening inhibitors of mutated influenza virus neuraminidases

Construction of a convenient system for easily screening inhibitors of mutated influenza virus neuraminidases

  • FEBS Open Bio. 2013 Oct 29;3:484-9. doi: 10.1016/j.fob.2013.10.007.
Tomohiro Shigemori 1,  Mitsuru Nagayama,  Junki Yamada,  Natsuko Miura,  Suganya Yongkiettrakul,  Kouichi Kuroda,  Tohoru Katsuragi,  Mitsuyoshi Ueda
Affiliations

Affiliation

  • 1 Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan.
PMID: 24265981 DOI: 10.1016/j.fob.2013.10.007
Abstract

Neuraminidase (NA) is a surface glycoprotein produced by the Influenza Virus. Specific NA mutations that confer resistance to anti-viral drugs have been reported. The aim of this study was to demonstrate quick preparation of the mutated NAs using the yeast surface display and its applicability for screening inhibitors. Plasmids encoding the head domain of wild-type and drug-resistant NAs were constructed and introduced into yeast, and these were successfully displayed on the yeast surface, with biochemical properties similar to the native virus NAs. This system using mutated NAs-displaying yeast provides an efficient and convenient tool for screening novel inhibitors against the drug-resistant Influenza Virus.

Keywords

Avian influenza virus H5N1; HNA, head domain of neuraminidase; Influenza A virus neuraminidase; NA, neuraminidase; Yeast surface display.

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