2. Academic Validation
  3. Prenylated isoflavones from Cudrania tricuspidata inhibit NO production in RAW 264.7 macrophages and suppress HL-60 cells proliferation

Prenylated isoflavones from Cudrania tricuspidata inhibit NO production in RAW 264.7 macrophages and suppress HL-60 cells proliferation

  • J Asian Nat Prod Res. 2017 May;19(5):510-518. doi: 10.1080/10286020.2016.1232253.
Hoang Le Tuan Anh 1,  Do Thanh Tuan 2,  Do Thi Trang 1,  Bui Huu Tai 1,  Nguyen Xuan Nhiem 1,  Pham Hai Yen 1,  Phan Van Kiem 1,  Chau Van Minh 1,  Tran Minh Duc 3,  Hee Kyoung Kang 4,  Youn Chul Kim 5,  Young Ho Kim 6
Affiliations

Affiliations

  • 1 a Institute of Marine Biochemistry , Vietnam Academy of Science and Technology , Hanoi , Vietnam.
  • 2 b Department of Biology , Thaibinh Medical University , Thaibinh , Vietnam.
  • 3 c Department of Anatomy , Vietnam University of Traditional Medicine , Hanoi , Vietnam.
  • 4 d School of Medicine and Institute of Medical Science, Jeju University , Jeju 690-756 , Korea.
  • 5 e College of Pharmacy, Wonkwang University , Iksan 570-749 , Korea.
  • 6 f College of Pharmacy, Chungnam National University , Daejeon 34134 , Korea.
PMID: 27649772 DOI: 10.1080/10286020.2016.1232253
Abstract

Inhibitory effects of NO production in RAW 264.7 macrophages guided the isolation of nine prenylated Isoflavones, including a new cudraisoflavone L (1) and eight known metabolites furowanin B (2), erysubin A (3), wighteone (4), lupalbigenin (5), laburnetin (6), isolupalbigenin (7), 6,8-diprenylorobol (8), millewanin H (9) from the leaves of Cudrania tricuspidata. At the concentration of 10 μM, compounds 1, 2, and 4 significantly inhibited NO production with the inhibitory values of 72.5 ± 2.4, 66.9 ± 1.8, and 55.4 ± 2.7%, respectively. In addition, all of isolated compounds 1-9 showed promising cytotoxic effects toward HL-60 cells (IC50 4.3 ± 0.7 to 18.0 ± 1.7 μM).

Keywords

Cudrania tricuspidata; NO inhibitor; Prenylated isoflavone; anti-leukemia; cudraisoflavone L.

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