2. Academic Validation
  3. Immunostimulatory effect of aqueous extract of Coriandrum sativum L. seed on macrophages

Immunostimulatory effect of aqueous extract of Coriandrum sativum L. seed on macrophages

  • J Sci Food Agric. 2017 Nov;97(14):4727-4736. doi: 10.1002/jsfa.8341.
Momoko Ishida 1,  Kosuke Nishi 1 2,  Nanami Kunihiro 1,  Hiroyuki Onda 3,  Sogo Nishimoto 4,  Takuya Sugahara 1 2 5
Affiliations

Affiliations

  • 1 Faculty of Agriculture, Ehime University, Matsuyama, Ehime, Japan.
  • 2 Food and Health Sciences Research Center, Ehime University, Matsuyama, Ehime, Japan.
  • 3 Research and Analysis Center, S&B Foods Inc., Tokyo, Japan.
  • 4 Department of Food Science, Ishikawa Prefectural University, Nonoichi, Ishikawa, Japan.
  • 5 South Ehime Fisheries Research Center, Ehime University, Ainan, Ehime, Japan.
PMID: 28369918 DOI: 10.1002/jsfa.8341
Abstract

Background: Coriandrum sativum L. seed is generally used as a spice and crude drug. Although many functions of the various components in C. sativum L. seed have been reported, the immunostimulatory effect of water-soluble components in C. sativum L. seed has not been studied. In the present study, we focused on the immunostimulatory effect of C. sativum L. seed aqueous extract (CAE) on macrophages as a novel health function of C. sativum L. seed components.

Results: CAE significantly enhanced the production of tumor necrosis factor (TNF)-α and interleukin (IL)-6 in both RAW264.7 cells and peritoneal macrophages by enhancing the expression levels of these cytokine genes. CAE also stimulated nitric oxide (NO) production and the phagocytosis activity in RAW264.7 cells. We suggest that the activity of CAE is a result of the upregulation of mitogen-activated protein kinase and nuclear factor-κB cascades via TLR4. In addition, IL-6 production by peritoneal macrophages collected from CAE-administered mice was significantly enhanced, suggesting that CAE could stimulate macrophage activity in vivo.

Conclusion: The findings of the present study suggest that CAE contains a novel water-soluble component with an immunostimulatory effect on macrophages. CAE would contribute to activating host defense against pathogens by stimulating the innate immunity. © 2017 Society of Chemical Industry.

Keywords

Coriandrum sativum L; RAW264.7 cells; immunostimulatory effect; peritoneal macrophages; phagocytosis activity.

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