2. Academic Validation
  3. Combination of Astragalus membranaceous and Angelica sinensis Ameliorates Vascular Endothelial Cell Dysfunction by Inhibiting Oxidative Stress

Combination of Astragalus membranaceous and Angelica sinensis Ameliorates Vascular Endothelial Cell Dysfunction by Inhibiting Oxidative Stress

  • Evid Based Complement Alternat Med. 2020 Sep 18;2020:6031782. doi: 10.1155/2020/6031782.
Yonghui Yin 1,  Hui Li 2,  Yumin Chen 3,  Ranran Zhu 1,  Li Li 1,  Xinying Zhang 1,  Ji Zhou 4,  Zichen Wang 4,  Xiaoli Li 5
Affiliations

Affiliations

  • 1 Department of Endocrinology, Hospital Affiliated to Shandong University of Traditional Chinese Medicine, Jinan 250011, China.
  • 2 Department of Emergency, Hospital Affiliated to Shandong University of Traditional Chinese Medicine, Jinan 250011, China.
  • 3 The Medical Record Room, Hospital Affiliated to Shandong University of Traditional Chinese Medicine, Jinan 250011, China.
  • 4 Shandong University of Traditional Chinese Medicine, Jinan 250011, China.
  • 5 Department of Editorial, Shandong University of Traditional Chinese Medicine, Jinan 250355, China.
PMID: 33293989 DOI: 10.1155/2020/6031782
Abstract

Vascular endothelial dysfunction is an essential and early sign of diabetic macroangiopathy, a primary complication of diabetes mellitus. Astragalus membranaceous-Angelica sinensis is a classic medical combination applied in China to treat diabetes mellitus. The aim of this study was to investigate the effect of the granule form of the extract produced from the dried root of Astragalus membranaceous (AM) combination with the granule form of the extract produced from the dried Angelica sinensis (AS) on diabetic macroangiopathy and its underlying mechanism. Herein, rats were treated by AM-AS at a ratio of 3 : 2 via intragastric administration. High glucose-induced human umbilical vein vascular endothelial cells (HUVECs) were then treated with drug-containing serum collected from the rats. In high glucose-treated HUVECs, AM-AS combination increased cell viability (P < 0.05), decreased the percentage of apoptotic cells (P < 0.05) and the expression of the proapoptosis protein Caspase 3 (P < 0.05), reduced the proportion of cells in the G0/G1 phase (P < 0.05), decreased Reactive Oxygen Species level (P < 0.05), enhanced cell migration and invasion (P < 0.05), and reduced the level of 8-iso-prostaglandin F2alpha. These results indicate that AM-AS combination at the ratio of 3 : 2 ameliorated HUVEC dysfunction by regulating Apoptosis, cell migration, and invasion, which might be mediated by their regulatory effect on Reactive Oxygen Species production. The current study provides a theoretical basis for the treatment of diabetic macroangiopathy using AM-AS.

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