1. Academic Validation
  2. Effects of Maxingloushi decoction on immune inflammation and programmed death markers in mice with chronic obstructive pulmonary disease

Effects of Maxingloushi decoction on immune inflammation and programmed death markers in mice with chronic obstructive pulmonary disease

  • World J Emerg Med. 2022;13(1):32-37. doi: 10.5847/wjem.j.1920-8642.2022.023.
Li Li 1 2 Jun Yan 1 2 Lin-Qin Ma 1 2 Wei Bi 1 2 Cai-Jun Wu 1 2
Affiliations

Affiliations

  • 1 Emergency Department, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China.
  • 2 Institute of Sepsis, Beijing University of Chinese Medicine, Beijing 100029, China.
Abstract

Background: To investigate effects of Maxingloushi decoction on lung inflammation and programmed death markers (programmed death-1 [PD-1], programmed death-ligand 1 [PD-L1]) in the lung tissue, peripheral blood, and bronchoalveolar lavage fluid (BLF) in a mouse model of chronic obstructive pulmonary disease (COPD).

Methods: Thirty-six mature male BALB/C mice were randomly divided into normal group (group A, n=6), COPD model group (group B, n=10), Maxingloushi decoction + COPD group (group C, n=10), and PD-1 inhibitor + COPD group (group D, n=10). The COPD model was established by smoke inhalation combined with lipopolysaccharide (LPS). Levels of PD-1 and PD-L1 in plasma and BLF were measured by enzyme-linked immunosorbent assay (ELISA). Histopathological techniques were used to semi-quantitatively analyze the immuno-fluorescence optical density (IOD) value of the lung tissue.

Results: In plasma and BLF, the expression of PD-1 in the group B was higher than that in the group A, and the expression of PD-L1 was lower than that in the group A. The expression of PD-1 and PD-L1 in the lung tissue was normalized in the group C in comparison with the group B (P<0.05) and the group D (P<0.05), and inflammatory cell infiltration in the lung tissue was also improved.

Conclusions: These findings reveal that COPD causes an immune imbalance in the peripheral blood and lung tissue, and that both Maxingloushi decoction and PD-1 inhibitor treatment can mitigate lung inflammation in COPD by reducing PD-1 expression and increasing PD-L1 expression. The treatment effect of Maxingloushi decoction may be superior to that of PD-1 inhibitor.

Keywords

Chronic obstructive pulmonary disease; Immune; Maxingloushi decoction; Mice; Programmed death-1; Programmed death-ligand 1.

Figures
Products