2. Academic Validation
  3. Aberrant BMP15/HIF-1α/SCF signaling pathway in human granulosa cells is involved in the PCOS related abnormal follicular development

Aberrant BMP15/HIF-1α/SCF signaling pathway in human granulosa cells is involved in the PCOS related abnormal follicular development

  • Gynecol Endocrinol. 2022 Sep 23;1-7. doi: 10.1080/09513590.2022.2125951.
Li-Yun Cao 1 2,  Zhi-Qin Zhang 1 2,  Pei-Pei Liu 1 2,  Ding-Fei Xu 1 2,  Liang Tang 1,  Lu Fan 1,  Xue-Chen Sun 1 3,  Jia-Yi Li 1 3,  Qiong-Fang Wu 1 3,  Zeng-Ming Li 2,  Jun Tan 1 2 3
Affiliations

Affiliations

  • 1 Jiangxi Provincial Maternal and Child Health Hospital, Reproductive Medicine Center, Maternal and Child Health Hospital Affiliated to Nanchang University, Nanchang, Jiangxi, P. R. China.
  • 2 JXHC Key Laboratory of Fertility Preservation, Jiangxi Maternal and Child Health Hospital, Nanchang, Jiangxi, P.R. China.
  • 3 Jiangxi Medical College, Nanchang University, Nanchang, Jiangxi, P. R. China.
PMID: 36151730 DOI: 10.1080/09513590.2022.2125951
Abstract

Aims: To investigate the regulatory mechanism of SCF expression in human GCs of PCOS related follicles.

Materials and methods: SCF, BMP15 and HIF-1α were evaluated in human serums, follicular fluids (FFs) and GCs, which were collected from 69 PCOS patients and 74 normal ovulatory patients. KGN cell line was used in this study.

Results: Our results showed that the rate of MII oocyte and 2PN fertilization was lower in PCOS group, though PCOS patients retrieved much more oocytes. The level of BMP15 in FF and the level of SCF in serum and FF were also lower in PCOS patients. We found a weakened expression of HIF-1α and SCF in GCs from PCOS patients when compared with the non-PCOS patients. The expression of HIF-1α and SCF was significantly increased in KGN cells after treating cells with rhBMP15, however, this promotion effects of BMP15 on HIF-1α and SCF expression were obviously abolished by co-treatment with BMP-I receptor inhibitor (DM). Moreover, knock down of HIF-1α expression in KGN cells significantly reduced the expression of SCF in human GCs, in spite of activating BMP15 signaling pathway.

Conclusions: The present study suggest that BMP15 could induce SCF expression by up-regulating HIF-1α expression in human GCs, the aberrance of this signaling pathway might be involved in the PCOS related abnormal follicular development.

Keywords

BMP15; HIF-1α; PCOS; SCF; granulosa cells.

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