2. Academic Validation
  3. The crosstalk between LINC01089 and hippo pathway inhibits osteosarcoma progression

The crosstalk between LINC01089 and hippo pathway inhibits osteosarcoma progression

  • J Bone Miner Metab. 2022 Nov 18. doi: 10.1007/s00774-022-01377-9.
Hao Zhang # 1 2 3,  Lin Zhou # 1 2,  Shaopu Hu 4,  Wei Gu 1,  Zhiqiang Li 1,  Jun Sun 1,  Xiaoen Wei 5,  Yongjun Wang 6
Affiliations

Affiliations

  • 1 Department of Orthopedics, Shuguang Hospital Affiliated to Shanghai University of TCM, Shanghai, 200000, China.
  • 2 Shi's Center of Orthopedics and Traumatology, Shuguang Hospital Affiliated to Shanghai University of TCM, Shanghai, 200120, China.
  • 3 Institute of Traumatology and Orthopedics, Shanghai Academy of Traditional Chinese Medicine, Shanghai, 200120, China.
  • 4 Department of Oncology, Dongfang Hospital Affiliated to Beijing University of TCM, Beijing, China.
  • 5 Department of Orthopedics, Shuguang Hospital Affiliated to Shanghai University of TCM, Shanghai, 200000, China. [email protected].
  • 6 Shanghai University of TCM, Shanghai, 200032, China. [email protected].
  • # Contributed equally.
PMID: 36399257 DOI: 10.1007/s00774-022-01377-9
Abstract

Introduction: Osteosarcoma is the most common malignancy in children, with high morbidity worldwide. Researches indicated that long non-coding RNAs (lncRNAs) played crucial roles in various cancers. Nevertheless, study investigating lncRNA long intergenic non-protein coding RNA 1089 (LINC01089) in osteosarcoma is extremely rare. Thus, the research of LINC01089 is of great significance.

Materials and methods: qRT-PCR and western blot were done to test the expression of RNAs and proteins in osteosarcoma cells. Functional assays were carried out to evaluate biological behaviors of hFOB1.19 and osteosarcoma cells with or without LINC01089 knockdown and overexpression. In vitro and in vivo experiments in a rescue manner were performed to reveal the influences of LINC01089 and Hippo pathway on osteosarcoma cell phenotype and tumor growth.

Results: LINC01089 was down-regulated in osteosarcoma cells and overexpressing LINC01089 was validated to restrain cell growth in vitro and tumor growth in vivo. Additionally, silencing LINC01089 could exacerbate cell malignant behaviors. Correlation of LINC01089 and Hippo pathway was proved. Overexpressing LINC01089 could activate Hippo pathway to exert antitumor effects.

Conclusion: LINC01089 could restrain the progression of osteosarcoma through activating Hippo pathway.

Keywords

Biological functions; Hippo pathway; LINC01089; Osteosarcoma.

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