2. Academic Validation
  3. Assays and Utilization of Enzymes Involved in Glycolipid Metabolism in Bacteria and Fungi

Assays and Utilization of Enzymes Involved in Glycolipid Metabolism in Bacteria and Fungi

  • Methods Mol Biol. 2023:2613:229-256. doi: 10.1007/978-1-0716-2910-9_18.
Makoto Ito 1,  Yohei Ishibashi 2,  Takashi Watanabe 2 3,  Jun Iwaki 4,  Toyohisa Kurita 5,  Nozomu Okino 6
Affiliations

Affiliations

  • 1 Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Fukuoka, Japan. [email protected].
  • 2 Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Fukuoka, Japan.
  • 3 Department of Pathophysiology and Metabolism, Kawasaki Medical School, Okayama, Japan.
  • 4 Tokyo Chemical Industry Co., Ltd., Tokyo, Japan.
  • 5 TAKARA BIO INC., Shiga, Japan.
  • 6 Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Fukuoka, Japan. [email protected].
PMID: 36587083 DOI: 10.1007/978-1-0716-2910-9_18
Abstract

Microbial glycosphingolipid (GSL)-degrading enzymes with unique specificity are useful tools for GSL research. On the other hand, some microbial glycolipids, not only GSLs but also steryl glucosides, are closely related to pathogenicity, and, thus, the metabolism of microbial glycolipids is attracting attention as a target for Antibiotics. This chapter describes the assays and utilization of microbial enzymes useful for glycolipid research and those involved in pathogenicity or host immune reactions.

Keywords

Bacteria; Fungi; Glycohydrolase; Glycolipid; Glycosyltransferase; N-deacylase.

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