1. Academic Validation
  2. Atractylodin Ameliorates Colitis via PPARα Agonism

Atractylodin Ameliorates Colitis via PPARα Agonism

  • Int J Mol Sci. 2023 Jan 2;24(1):802. doi: 10.3390/ijms24010802.
Gwangbeom Heo 1 Yuju Kim 1 Eun-La Kim 1 Soyeong Park 1 Sang Hoon Rhee 2 Jee H Jung 1 3 Eunok Im 1 3
Affiliations

Affiliations

  • 1 College of Pharmacy, Pusan National University, Busan 46241, Republic of Korea.
  • 2 Department of Biological Sciences, Oakland University, Rochester, MI 48309, USA.
  • 3 Research Institute for Drug Development, Pusan National University, Busan 46241, Republic of Korea.
Abstract

Atractylodin is a major compound in the rhizome of Atractylodes lancea, an oriental herbal medicine used for the treatment of gastrointestinal diseases, including dyspepsia, nausea, and diarrhea. Recent studies have shown that atractylodin exerts anti-inflammatory effects in various inflammatory diseases. Herein, we investigated the anti-colitis effects of atractylodin and its molecular targets. We determined the non-cytotoxic concentration of atractylodin (50 μM) using a cell proliferation assay in colonic epithelial cells. We found that pretreatment with atractylodin significantly inhibits tumor necrosis factor-α-induced phosphorylation of nuclear factor-κ-light-chain-enhancer of activated B in HCT116 cells. Through docking simulation analysis, luciferase assays, and in vitro binding assays, we found that atractylodin has an affinity for Peroxisome Proliferator-activated Receptor alpha (PPARα). Daily administration of atractylodin (40 mg/kg) increased the survival rate of mice in a dextran sodium sulfate-induced colitis mouse model. Thus, atractylodin can be a good strategy for colitis therapy through inducing PPARα-dependent pathways.

Keywords

Atractylodes lancea; PPAR alpha; atractylodin; inflammatory bowel disease.

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