2. Academic Validation
  3. Quantification of Cardiomyocyte Contraction In Vitro and Drug Screening by MyocytoBeats

Quantification of Cardiomyocyte Contraction In Vitro and Drug Screening by MyocytoBeats

  • J Cardiovasc Transl Res. 2023 Jan 30. doi: 10.1007/s12265-023-10357-x.
Zhiyang Cheng # 1,  Yuxin Yang # 1,  Kai Jiang 1,  Hongyi Nie 2,  Xingbo Yang 1,  Zizhuo Tu 1,  Jiayi Liang 3,  Yaozu Xiang 4
Affiliations

Affiliations

  • 1 Department of Obstetrics and Gynecology, Shanghai Fourth People's Hospital, School of Life and Sciences and Technology, Tongji University, Shanghai, 200092, China.
  • 2 School of Mechanical Engineering and the School of Artificial Intelligence, Optics and Electronics (iOPEN), Northwestern Polytechnical University, Xi'an, China.
  • 3 Department of Epidemiology and Biostatistics, School of Public Health, Imperial College London, London, UK.
  • 4 Department of Obstetrics and Gynecology, Shanghai Fourth People's Hospital, School of Life and Sciences and Technology, Tongji University, Shanghai, 200092, China. [email protected].
  • # Contributed equally.
PMID: 36715820 DOI: 10.1007/s12265-023-10357-x
Abstract

Cardiomyocyte contractility is the crucial feature of heart function. Quantifying cardiomyocyte contraction in vitro is essential for disease phenotype characterization, mechanism illumination, and drug screening. Although many experimental methods have been employed to determine contraction dynamics in vitro, a time-saving and easy-to-use software is still needed to be developed. We presented a reliable tool, named MyocytoBeats, to measure cardiomyocyte contraction by processing recorded videos. Analysis results by MyocytoBeats of various experimental models have shown a significant linear relationship with another validated software. We also performed pharmacology screen in the platform, and astragaloside IV was identified to stabilize the frequency and amplitude of cardiomyocyte in the arrhythmia model. MyocytoBeats is a high-performance tool for generating cardiomyocyte contraction data of vitro study and shows a great potential in cardiac pharmacology study.

Keywords

Cardiac; Contraction; Drug screening; Software.

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