2. Academic Validation
  3. Wulfenioidins D-N, Structurally Diverse Diterpenoids with Anti-Zika Virus Activity Isolated from Orthosiphon wulfenioides

Wulfenioidins D-N, Structurally Diverse Diterpenoids with Anti-Zika Virus Activity Isolated from Orthosiphon wulfenioides

  • J Nat Prod. 2023 Sep 22. doi: 10.1021/acs.jnatprod.3c00543.
Wen-Chao Tu 1 2,  Yong-Xiang Huang 3,  Bo Li 2,  Ying-Jie Jiang 3,  Quan-Yu Yang 2,  Muhammad Aurang Zeb 2,  Peng-Yun Yang 2,  Hui-Juan Wang 1,  Xiao-Li Li 2,  Wei-Lie Xiao 2,  Chang-Bo Zheng 3,  Mei-Feng Liu 1
Affiliations

Affiliations

  • 1 Key Laboratory of Functional Molecular Engineering of Guangdong Province, School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou, 510640, People's Republic of China.
  • 2 Key Laboratory of Medicinal Chemistry for Natural Resource, Ministry of Education, Yunnan Characteristic Plant Extraction Laboratory, Yunnan Provincial Center for Research & Development of Natural Products, State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, School of Pharmacy and School of Chemical Science and Technology, Yunnan University, Kunming, 650091, People's Republic of China.
  • 3 School of Pharmaceutical Science and Yunnan Key Laboratory of Pharmacology for Natural Products, Yunnan Vaccine Laboratory, Kunming Medical University, Kunming, 650500, People's Republic of China.
PMID: 37737089 DOI: 10.1021/acs.jnatprod.3c00543
Abstract

Eleven Diterpenoids, wulfenioidins D-N (1-11), classified into five distinct carbon skeletons with one unreported framework, and four modified abietane Diterpenoids were isolated from the whole plant of Orthosiphon wulfenioides. The structures and absolute configurations were characterized by spectroscopic methods, single-crystal X-ray diffraction, and electronic circular dichroism analyses. Compounds 3 and 5 exhibited activity against Zika virus (ZIKV) with EC50 values of 8.07 and 8.50 μM, respectively, and showed no significant cytotoxicity toward Vero cells at 100 μM. Western blot and immunofluorescence experiments showed that compounds 3 and 5 interfered with the replication of the ZIKV by inhibiting the expression of the ZIKV envelope (E) protein.

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