2. Academic Validation
  3. Casein kinase 2 phosphorylates and induces the SALL2 tumor suppressor degradation in colon cancer cells

Casein kinase 2 phosphorylates and induces the SALL2 tumor suppressor degradation in colon cancer cells

  • Cell Death Dis. 2024 Mar 16;15(3):223. doi: 10.1038/s41419-024-06591-z.
V E Hermosilla 1 2 3,  L Gyenis 4,  A J Rabalski 4 5,  M E Armijo 1 2,  P Sepúlveda 1 2,  F Duprat 6,  D Benítez-Riquelme 1 2,  F Fuentes-Villalobos 1 2 7,  A Quiroz 1 2,  M I Hepp 1 2 8,  C Farkas 1 2 8,  M Mastel 9 10,  I González-Chavarría 6,  R Jackstadt 9 10,  D W Litchfield 4,  A F Castro 11 12,  R Pincheira 13 14
Affiliations

Affiliations

  • 1 Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias Biológicas, Universidad de Concepción, Concepción, Chile.
  • 2 Laboratorio de Transducción de Señales y Cáncer, Facultad de Ciencias Biológicas, Universidad de Concepción, Concepción, Chile.
  • 3 Dept of Orofacial Sciences and Dept of Anatomy, University of California-San Francisco, San Francisco, CA, USA.
  • 4 Department of Biochemistry, Schulich School of Medicine & Dentistry, University of Western Ontario, London, ON, Canada.
  • 5 Odyssey Therapeutics, Boston, MA, USA.
  • 6 Departamento de Fisiopatología, Facultad de Ciencias Biológicas, Universidad de Concepción, Concepción, Chile.
  • 7 Laboratorio de Inmunovirología. Departamento de Microbiologia. Facultad de Ciencias Biológicas, Universidad de Concepción, Concepción, Chile.
  • 8 Laboratorio de Investigación en Ciencias Biomédicas, Departamento de Ciencias Básicas y Morfología, Facultad de Medicina, Universidad Católica de la Santísima Concepción, Concepción, Chile.
  • 9 Heidelberg Institute for Stem Cell Technology and Experimental Medicine (HI-STEM gGmbH), 69120 Heidelberg. Cancer Progression and Metastasis Group, German Cancer Research Center (DKFZ) and DKFZ-ZMBH Alliance, 69120, Heidelberg, Germany.
  • 10 Faculty of Biosciences, Heidelberg University, 69120, Heidelberg, Germany.
  • 11 Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias Biológicas, Universidad de Concepción, Concepción, Chile. [email protected].
  • 12 Laboratorio de Transducción de Señales y Cáncer, Facultad de Ciencias Biológicas, Universidad de Concepción, Concepción, Chile. [email protected].
  • 13 Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias Biológicas, Universidad de Concepción, Concepción, Chile. [email protected].
  • 14 Laboratorio de Transducción de Señales y Cáncer, Facultad de Ciencias Biológicas, Universidad de Concepción, Concepción, Chile. [email protected].
PMID: 38493149 DOI: 10.1038/s41419-024-06591-z
Abstract

Spalt-like proteins are Zinc finger transcription factors from Caenorhabditis elegans to vertebrates, with critical roles in development. In vertebrates, four paralogues have been identified (SALL1-4), and SALL2 is the family's most dissimilar member. SALL2 is required during brain and eye development. It is downregulated in Cancer and acts as a tumor suppressor, promoting cell cycle arrest and cell death. Despite its critical functions, information about SALL2 regulation is scarce. Public data indicate that SALL2 is ubiquitinated and phosphorylated in several residues along the protein, but the mechanisms, biological consequences, and enzymes responsible for these modifications remain unknown. Bioinformatic analyses identified several putative phosphorylation sites for Casein Kinase II (CK2) located within a highly conserved C-terminal PEST degradation motif of SALL2. CK2 is a serine/threonine kinase that promotes cell proliferation and survival and is often hyperactivated in Cancer. We demonstrated that CK2 phosphorylates SALL2 residues S763, T778, S802, and S806 and promotes SALL2 degradation by the Proteasome. Accordingly, pharmacological inhibition of CK2 with Silmitasertib (CX-4945) restored endogenous SALL2 protein levels in SALL2-deficient breast MDA-MB-231, lung H1299, and colon SW480 Cancer cells. Silmitasertib induced a methuosis-like phenotype and cell death in SW480 cells. However, the phenotype was significantly attenuated in CRISPr/Cas9-mediated SALL2 knockout SW480 cells. Similarly, Sall2-deficient tumor organoids were more resistant to Silmitasertib-induced cell death, confirming that SALL2 sensitizes Cancer cells to CK2 inhibition. We identified a novel CK2-dependent mechanism for SALL2 regulation and provided new insights into the interplay between these two proteins and their role in cell survival and proliferation.

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