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  4. Methimazole

Methimazole  (Synonyms: Thiamazole)

Cat. No.: HY-B0208 Purity: 99.96%
COA Handling Instructions

Molarity Calculator

Methimazole is an antithyroid compound widely used for the research of hyperthyroidism. Methimazole has potent hepatotoxicity.

For research use only. We do not sell to patients.

Methimazole Chemical Structure

Methimazole Chemical Structure

CAS No. : 60-56-0

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Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 61 In-stock
Solution
10 mM * 1 mL in DMSO USD 61 In-stock
Solid
500 mg USD 55 In-stock
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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Methimazole:

Methimazole Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Methimazole

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Methimazole is an antithyroid compound widely used for the research of hyperthyroidism. Methimazole has potent hepatotoxicity[1][2].

In Vitro

Methimazole inhibits CXC chemokine ligand 10 secretion in human thyrocytes[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Methimazole Related Antibodies
Clinical Trial
Molecular Weight

114.17

Formula

C4H6N2S
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

S=C1NC=CN1C
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : ≥ 100 mg/mL (875.89 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : ≥ 50 mg/mL (437.94 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 8.7589 mL 43.7943 mL 87.5887 mL
5 mM 1.7518 mL 8.7589 mL 17.5177 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (21.90 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (21.90 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 100 mg/mL (875.89 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 99.96%

COA (252 KB) HNMR (219 KB) LCMS (257 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 8.7589 mL 43.7943 mL 87.5887 mL 218.9717 mL
5 mM 1.7518 mL 8.7589 mL 17.5177 mL 43.7943 mL
10 mM 0.8759 mL 4.3794 mL 8.7589 mL 21.8972 mL
15 mM 0.5839 mL 2.9196 mL 5.8392 mL 14.5981 mL
20 mM 0.4379 mL 2.1897 mL 4.3794 mL 10.9486 mL
25 mM 0.3504 mL 1.7518 mL 3.5035 mL 8.7589 mL
30 mM 0.2920 mL 1.4598 mL 2.9196 mL 7.2991 mL
40 mM 0.2190 mL 1.0949 mL 2.1897 mL 5.4743 mL
50 mM 0.1752 mL 0.8759 mL 1.7518 mL 4.3794 mL
60 mM 0.1460 mL 0.7299 mL 1.4598 mL 3.6495 mL
80 mM 0.1095 mL 0.5474 mL 1.0949 mL 2.7371 mL
100 mM 0.0876 mL 0.4379 mL 0.8759 mL 2.1897 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Methimazole Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Methimazole60-56-0ThiamazoleOthersantithyroidhepatotoxicityhepatocytescytotoxicityInhibitorinhibitorinhibit

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