2. Protein Tyrosine Kinase/RTK Autophagy
  3. Bcr-Abl FGFR PDGFR VEGFR Src Autophagy
  4. Ponatinib hydrochloride

Ponatinib hydrochloride  (Synonyms: AP24534 hydrochloride)

Cat. No.: HY-108766 Purity: 99.55%
COA Handling Instructions

Molarity Calculator

Ponatinib hydrochloride (AP24534 hydrochloride) is a hydrochloride of ponatinib. Ponatinib is an orally active multi-targeted kinase inhibitor with IC50s of 0.37 nM, 1.1 nM, 1.5 nM, 2.2 nM, and 5.4 nM for Abl, PDGFRα, VEGFR2, FGFR1, and Src, respectively.

For research use only. We do not sell to patients.

Ponatinib hydrochloride Chemical Structure

Ponatinib hydrochloride Chemical Structure

CAS No. : 1114544-31-8

Size Price Stock Quantity
Free Sample (0.1 - 0.5 mg)   Apply Now  
5 mg USD 32 In-stock
10 mg USD 50 In-stock
25 mg USD 90 In-stock
50 mg USD 140 In-stock
100 mg USD 210 In-stock
200 mg   Get quote  
500 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 26 publication(s) in Google Scholar

Other Forms of Ponatinib hydrochloride:

Ponatinib hydrochloride Related Antibodies

Top Publications Citing Use of Products

    Ponatinib hydrochloride purchased from MedChemExpress. Usage Cited in: Exp Hematol. 2014 May;42(5):369-379.e3.  [Abstract]

    (B,D) Phosphorylation levels of F/P (or its mutant), STAT5, or ERK in lysates of CMPs expressing Hes1 with F/P, F/P-D842V, or F/P-T674I, which have been treated with indicated doses of (B) Imatinib or (D) Ponatinib for 15 min. Data are representative of three independent experiments.

    View All FGFR Isoform Specific Products:

    View All Isoforms

    View All PDGFR Isoform Specific Products:

    View All Isoforms

    View All VEGFR Isoform Specific Products:

    View All Isoforms

    View All Src Isoform Specific Products:

    View All Isoforms

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Ponatinib hydrochloride (AP24534 hydrochloride) is a hydrochloride of ponatinib. Ponatinib is an orally active multi-targeted kinase inhibitor with IC50s of 0.37 nM, 1.1 nM, 1.5 nM, 2.2 nM, and 5.4 nM for Abl, PDGFRα, VEGFR2, FGFR1, and Src, respectively[1].

    IC50 & Target

    LYN

    0.24 nM (IC50)

    Abl

    0.37 nM (IC50)

    PDGFRα

    1.1 nM (IC50)

    VEGFR2

    1.5 nM (IC50)

    FGFR1

    2.2 nM (IC50)

    Src

    5.4 nM (IC50)

    In Vitro

    Ponatinib hydrochloride (0.11, 017 μM, 24-48 h) can reduce the viability, migration, and function of human endothelial cells[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Ponatinib hydrochloride Related Antibodies

    Cell Viability Assay[2]

    Cell Line: HUVEC
    Concentration: 0-20 μM
    Incubation Time: 48 h
    Result: Decreased cell viability in a dose-dependent manner with IC50 value of 0.55 μM.

    Apoptosis Analysis[2]

    Cell Line: HUVEC
    Concentration: 0.11, 0.17 μM
    Incubation Time: 24 h
    Result: Increased the percentage of annexin/PI labeled cells.

    Cell Migration Assay [2]

    Cell Line: HUVEC
    Concentration: 0.17 μM
    Incubation Time: 20 h
    Result: Reduced VEGF-induced migration from 100% to 62.3 ± 4.8%.
    In Vivo

    Ponatinib hydrochloride (dissolved in 25 mM citrate buffer (pH 2.75), 200 μL in volume, oral) protects mice from lethal influenza infection by inhibiting cytokine storm[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Influenza A Virus Infected Mice[3]
    Dosage: 200 μL, dissolved in 25 mM citrate buffer (pH 2.75)
    Administration: p.o.
    Result: Had higher survival rates and reduced the infiltration of neutrophils.
    Clinical Trial
    Molecular Weight

    569.02

    Formula

    C29H28ClF3N6O
    CAS No.
    Appearance

    Solid

    Color

    Off-white to light yellow

    SMILES

    O=C(C1=CC=C(C)C(C#CC2=CN=C3C=CC=NN23)=C1)NC4=CC=C(CN5CCN(CC5)C)C(C(F)(F)F)=C4.Cl
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    Solvent & Solubility
    In Vitro: 

    DMSO : 25 mg/mL (43.94 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.7574 mL 8.7870 mL 17.5741 mL
    5 mM 0.3515 mL 1.7574 mL 3.5148 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (4.39 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (4.39 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.55%

    COA (256 KB) HNMR (284 KB) LCMS (95 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 1.7574 mL 8.7870 mL 17.5741 mL 43.9352 mL
    5 mM 0.3515 mL 1.7574 mL 3.5148 mL 8.7870 mL
    10 mM 0.1757 mL 0.8787 mL 1.7574 mL 4.3935 mL
    15 mM 0.1172 mL 0.5858 mL 1.1716 mL 2.9290 mL
    20 mM 0.0879 mL 0.4394 mL 0.8787 mL 2.1968 mL
    25 mM 0.0703 mL 0.3515 mL 0.7030 mL 1.7574 mL
    30 mM 0.0586 mL 0.2929 mL 0.5858 mL 1.4645 mL
    40 mM 0.0439 mL 0.2197 mL 0.4394 mL 1.0984 mL
    • No file chosen (Maximum size is: 1024 Kb)
    • If you have published this work, please enter the PubMed ID.
    • Your name will appear on the site.

    Ponatinib hydrochloride Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Ponatinib1114544-31-8AP24534AP 24534AP-24534Bcr-AblFGFRPDGFRVEGFRSrcAutophagyFibroblast growth factor receptorPlatelet-derived growth factor receptorVascular endothelial growth factor receptororally activemulti-targetedkinaseAblPDGFRαVEGFR2FGFR1Inhibitorinhibitorinhibit

    Your Recently Viewed Products:

    Inquiry Online

    Your information is safe with us. * Required Fields.

    Product Name

    		  

    Salutation

    Applicant Name *

    		 

    Email Address *

    Phone Number *

    		 

    Organization Name *

    Department *

    		 

    Requested quantity *

    Country or Region *

    		      

    Remarks

    Bulk Inquiry

    Inquiry Information

    Product Name:
    Ponatinib hydrochloride
    Cat. No.:
    HY-108766
    Quantity:
    MCE Japan Authorized Agent:

    Customer Review

    Thank You for Your Feedback!

    Request for HNMR Report

    Please fill out this form to request the QC report. We will send it to your Email address shortly.

    Your information is safe with us. * Required Fields.

    Product Name

    		  

    Lot #

    Applicant Name *

    		 

    Email Address *

    Phone Number

    		 

    Department *

    Organization Name *

    		 

    Country or Region *

    Remarks

    Request for HNMR Report

    We have received your request and will respond to you as soon as possible.