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Enzymes can be classified systematically according to the difference between reaction and substrate specificity, and the mechanism of action. Ligases (EC 6.x.x.x) are a group of enzymes that catalyze the reaction of joining two large molecules (such as proteins or peptides) by establishing a new chemical bond, for instance C-O, C-N, C-S bonds. Ligases are further divided into six subclasses: 1) EC 6.1 ligases establishing carbon-oxygen bonds; 2) EC 6.2 ligases establishing carbon-sulfur bonds; 3) EC 6.3 ligases establishing carbon-nitrogen bonds; 4) EC 6.4 ligases establishing carbon-carbon bonds; 5) EC 6.5 ligases establishing phosphoric-ester bonds; and 6) EC 6.6 ligases establishing nitrogen-metal bonds. DNA ligase is a common ligase which often used in molecular biology laboratories to join DNA fragments.
The KARS protein promotes attachment of amino acids to its cognate tRNA through a two-step reaction, inducing immune responses through monocyte/macrophage activation. In microbial infections, it interacts with the HIV-1 GAG protein to enable selective tRNA(3)(Lys) packaging to initiate reverse transcription. KARS Protein, Human (HEK293, His) is the recombinant human-derived KARS protein, expressed by HEK293 , with C-6*His labeled tag. The total length of KARS Protein, Human (HEK293, His) is 596 a.a., with molecular weight of 70-90 kDa.
GLUL proteins catalyze the conversion of glutamate and ammonia to glutamine. Glutamine synthetase/GLUL Protein, Human (His) is the recombinant human-derived Glutamine synthetase/GLUL protein, expressed by E. coli , with C-6*His labeled tag. The total length of Glutamine synthetase/GLUL Protein, Human (His) is 372 a.a., with molecular weight of 40-50 kDa.
Glutathione synthase/GSS protein catalyzes the ATP-dependent synthesis of glutathione from gamma-glutamylcysteine and glycine. Glutathione synthetase/GSS Protein, Human (His) is the recombinant human-derived Glutathione synthetase/GSS protein, expressed by E. coli , with C-6*His labeled tag. The total length of Glutathione synthetase/GSS Protein, Human (His) is 473 a.a., with molecular weight of ~55.0 kDa.
Acetyl-CoA synthetase 1/AceCS Protein, Mouse (His) is an enzyme that converts acetate to acetyl-CoA, which supplies acetyl-CoA for tumor cells by capturing acetate as a carbon source under stressed conditions.
DARS Protein, a central player, catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP, maintaining cellular energy homeostasis. It also exhibits nucleoside diphosphate kinase activity, producing various nucleoside triphosphates. At a low rate, DARS participates in thiamine triphosphate synthesis from thiamine diphosphate and ADP, showcasing its multifaceted role in nucleotide metabolism. DARS Protein, Human (His) is the recombinant human-derived DARS protein, expressed by E. coli , with N-His labeled tag. The total length of DARS Protein, Human (His) is 501 a.a., with molecular weight of ~47 kDa.
BirA protein is not mentioned in the provided paragraph. If you have another paragraph or topic you'd like summarized with BirA as the subject, please provide it, and I'll be happy to assist. BirA Protein, E. coli (His-MBP) is the recombinant E. coli-derived BirA protein, expressed by E. coli, with N-His, N-MBP labeled tag. The total length of BirA Protein, E. coli (His-MBP) is 321 a.a., with molecular weight of 64-68 kDa.
rHuASS1, His; Argininosuccinate Synthase; Citrulline--Aspartate Ligase; ASS1; ASS
Human
E. coli
ASS1 Protein, Human (His) is a recombinant human Argininosuccinate synthase expressed in E. coli with a His tag. Argininosuccinate synthase is an enzyme that catalyzes the synthesis of argininosuccinate from citrulline and aspartate.
AARS1, a pivotal enzyme in protein synthesis, plays a crucial role in the attachment of alanine to tRNA(Ala) through a two-step process. Initially, alanine undergoes activation by ATP to form Ala-AMP, and subsequently, it is transferred to the acceptor end of tRNA(Ala). Beyond its primary aminoacylation function, AARS1 boasts an editing domain that enables the correction of incorrectly charged tRNA(Ala), underscoring its role in maintaining the fidelity of the translation process. This dual functionality highlights AARS1's significance in ensuring the accuracy and integrity of the protein synthesis machinery. AARS1 Protein, Mouse (sf9, His) is the recombinant mouse-derived AARS1 protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of AARS1 Protein, Mouse (sf9, His) is 968 a.a., with molecular weight of ~108 kDa.
The CPS1/CPSase I protein is critical in participating in the urea cycle and is essential for the elimination of excess ammonia in urea-bearing animals. This essential enzyme converts toxic ammonia (a byproduct of amino acid catabolism) into urea, promoting safe excretion. CPS1/CPSase I Protein, Human (His) is the recombinant human-derived CPS1/CPSase I protein, expressed by E. coli , with N-His labeled tag. The total length of CPS1/CPSase I Protein, Human (His) is 147 a.a., with molecular weight of ~20.5 kDa.
Gamma-GCS protein is a key enzyme in sulfur metabolism and initiates glutathione synthesis by catalyzing the incorporation of L-cysteine and L-glutamic acid. This important molecule critical for cellular antioxidant defense is synthesized through a critical 1/2 step facilitated by γ-GCS. Gamma-GCS Protein, E.coli (His-SUMO) is the recombinant E. coli-derived Gamma-GCS protein, expressed by E. coli , with N-His, N-SUMO labeled tag. The total length of Gamma-GCS Protein, E.coli (His-SUMO) is 518 a.a., with molecular weight of ~74.3 kDa.
HARS, a pivotal enzyme, orchestrates the ATP-dependent ligation of histidine to the 3'-end of its corresponding tRNA by forming an aminoacyl-adenylate intermediate (His-AMP). This critical step in protein synthesis ensures the accurate incorporation of histidine into nascent polypeptide chains. Beyond its fundamental role in translation, HARS also emerges as a multifunctional player, contributing to axon guidance processes. The involvement of HARS in axon guidance highlights its participation in broader cellular functions, extending its significance beyond its canonical role in aminoacylation. HARS/HisRS Protein, Human (sf9, His) is the recombinant human-derived HARS/HisRS protein, expressed by Sf9 insect cells , with N-His labeled tag. The total length of HARS/HisRS Protein, Human (sf9, His) is 508 a.a., with molecular weight of ~59.6 kDa.
GARS, a pivotal enzyme, facilitates the ATP-dependent ligation of glycine to the 3'-end of its corresponding tRNA through the formation of an aminoacyl-adenylate intermediate (Gly-AMP). This process is crucial for protein synthesis and maintaining the fidelity of the genetic code. Additionally, GARS exhibits a unique capability to generate diadenosine tetraphosphate (Ap4A), a universal signaling molecule involved in diverse cell regulation pathways, by directly condensing two ATP molecules. This dual enzymatic activity underscores the multifaceted role of GARS in both fundamental cellular processes, such as protein synthesis, and the modulation of signaling cascades through Ap4A, suggesting its potential significance in Ap4A homeostasis. GARS Protein, Human (sf9, His) is the recombinant human-derived GARS protein, expressed by Sf9 insect cells , with N-His labeled tag. The total length of GARS Protein, Human (sf9, His) is 739 a.a., with molecular weight of ~85.5 kDa.
AACS proteins play a key role in cellular metabolism by converting acetoacetate in the cytoplasm into acetoacetyl-CoA (by similarity). As a ketone body utilizing enzyme, AACS helps synthesize essential cellular components including cholesterol and fatty acids (by similarity). AACS Protein, Human (sf9, His) is the recombinant human-derived AACS protein, expressed by Sf9 insect cells , with N-His labeled tag. The total length of AACS Protein, Human (sf9, His) is 672 a.a., with molecular weight of ~60 kDa.
The Alanyl-tRNA synthetase protein facilitates a two-step process, activating alanine with ATP to form Ala-AMP and transferring it to the acceptor end of tRNA(Ala). Additionally, it corrects incorrectly charged tRNA(Ala) through its editing domain. Alanyl-tRNA synthetase Protein, Human (sf9, His) is the recombinant human-derived Alanyl-tRNA synthetase protein, expressed by Sf9 insect cells , with N-His labeled tag. The total length of Alanyl-tRNA synthetase Protein, Human (sf9, His) is 968 a.a., with molecular weight of ~109.2 kDa.
UBA6; Ubiquitin-like modifier-activating enzyme 6; Ubiquitin-activating enzyme 6; Monocyte protein 4; MOP-4; Ubiquitin-activating enzyme E1-like protein 2; E1-L2
Human
Sf9 insect cells
The ubiquitin E1 enzyme initiates ubiquitination by adenylating ubiquitin with ATP and forming a thioester bond with the E1 enzyme. It exhibits specificity for ubiquitin and excludes ubiquitin-like peptides. UBE1L2/UBA6 Protein, Human (Sf9, His, Strep) is the recombinant human-derived UBE1L2/UBA6 protein, expressed by Sf9 insect cells , with N-Strep, N-8*His labeled tag. The total length of UBE1L2/UBA6 Protein, Human (Sf9, His, Strep) is 1051 a.a., .
ACSS2; Acetyl-coenzyme A synthetase; cytoplasmic; Acetate--CoA ligase; Acetyl-CoA synthetase; ACS; AceCS; Acetyl-CoA synthetase 1; AceCS1; Acyl-CoA synthetase short-chain family member 2; Acyl-activating enzyme; Propionate--CoA ligase
Human
E. coli
The ACSS2 protein plays a key role in catalyzing the synthesis of acetyl-CoA from short-chain fatty acids, with acetate being the preferred substrate. Although acetate is efficiently utilized, ACSS2 can also process propionate, albeit with significantly lower affinity. ACSS2 Protein, Human is the recombinant human-derived ACSS2 protein, expressed by E. coli , with tag free. The total length of ACSS2 Protein, Human is 700 a.a., .
ACSS2; Acetyl-coenzyme A synthetase; cytoplasmic; Acetate--CoA ligase; Acetyl-CoA synthetase; ACS; AceCS; Acetyl-CoA synthetase 1; AceCS1; Acyl-CoA synthetase short-chain family member 2; Acyl-activating enzyme; Propionate--CoA ligase
Human
E. coli
The ACSS2 protein plays a key role in catalyzing the synthesis of acetyl-CoA from short-chain fatty acids, with acetate being the preferred substrate. Although acetate is efficiently utilized, ACSS2 can also process propionate, albeit with significantly lower affinity. ACSS2 Protein, Human (His) is the recombinant human-derived ACSS2 protein, expressed by E. coli , with N-6*His labeled tag. The total length of ACSS2 Protein, Human (His) is 700 a.a., .
