Animal-Free IL-31 Protein, Human (His)

IL-31, a multifunctional cytokine, activates STAT3, and potentially STAT1 and STAT5, through the IL-31 heterodimeric receptor (IL31RA and OSMR). Known for its involvement in skin immunity, IL-31 modulates immune responses. Beyond immunity, IL-31 enhances myeloid progenitor cell survival, indicating roles in diverse cellular processes. Moreover, IL-31 induces key gene expression, including RETNLA and serum amyloid A protein, in macrophages, suggesting broader impacts on inflammatory and immune-related pathways. Animal-Free IL-31 Protein, Human (His) is the recombinant human-derived animal-FreeIL-31 protein, expressed by E. coli, with C-His labeled tag. The total length of Animal-Free IL-31 Protein, Human (His) is 141 a.a., with molecular weight of ~16.78 kDa.

IL-31, a cytokine with diverse functions, acts by activating STAT3, and potentially STAT1 and STAT5, utilizing the IL-31 heterodimeric receptor formed by IL31RA and OSMR. This activation mechanism has been implicated in skin immunity, highlighting its role in modulating immune responses. Additionally, IL-31 exhibits the capacity to enhance myeloid progenitor cell survival in vitro, emphasizing its involvement in cellular processes beyond immune regulation. Furthermore, IL-31 induces the expression of key genes, such as RETNLA and serum amyloid A protein, in macrophages, suggesting a broader impact on inflammatory and immune-related pathways.

Measured by its ability to induce STAT3 activation in U-87 cells. The ED50 for this effect is <20 ng/mL.

Human

E. coli

C-His

Q6EBC2 (S24-T164)

386653  [NCBI]

MSHTLPVRLLRPSDDVQKIVEELQSLSKMLLKDVEEEKGVLVSQNYTLPCLSPDAQPPNNIHSPAIRAYLKTIRQLDNKSVIDEIIEHLDKLIFQDAPETNISVPTDTHECKRFILTISQQFSECMDLALKSLTSGAQQATT

Approximately 16.78 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a solution containing 1X PBS, pH 8.0.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.