PRCP Protein, Human (HEK293, His)

PRCP protein acts as an enzyme that cleaves the C-terminal amino acid linked to proline in peptides, including angiotensin II, III and des-Arg9-bradykinin. Although some substrates retain enzymatic activity even at neutral pH, this cleavage activity is noteworthy because it occurs at acidic pH. PRCP Protein, Human (HEK293, His) is the recombinant human-derived PRCP protein, expressed by HEK293 , with C-6*His labeled tag. The total length of PRCP Protein, Human (HEK293, His) is 475 a.a., with molecular weight of 60-90 kDa.

PRCP, or prolylcarboxypeptidase, is an enzyme known for its role in selectively cleaving C-terminal amino acids linked to proline in peptides like angiotensin II, III, and des-Arg9-bradykinin. A distinctive feature of PRCP is its preference for acidic pH during cleavage; however, it retains enzymatic activity with specific substrates even under neutral pH conditions. This dual pH responsiveness implies a versatile functionality for PRCP in different cellular environments, influencing the processing and bioactivity of peptides involved in essential physiological pathways, particularly those associated with the renin-angiotensin and kinin systems. The ability of PRCP to operate across a range of pH levels suggests its intricate regulatory role in peptide metabolism and signaling.

Human

HEK293

C-6*His

P42785 (L22-H496)

5547  [NCBI]

LRPALRALGSLHLPTNPTSLPAVAKNYSVLYFQQKVDHFGFNTVKTFNQRYLVADKYWKKNGGSILFYTGNEGDIIWFCNNTGFMWDVAEELKAMLVFAEHRYYGESLPFGDNSFKDSRHLNFLTSEQALADFAELIKHLKRTIPGAENQPVIAIGGSYGGMLAAWFRMKYPHMVVGALAASAPIWQFEDLVPCGVFMKIVTTDFRKSGPHCSESIHRSWDAINRLSNTGSGLQWLTGALHLCSPLTSQDIQHLKDWISETWVNLAMVDYPYASNFLQPLPAWPIKVVCQYLKNPNVSDSLLLQNIFQALNVYYNYSGQVKCLNISETATSSLGTLGWSYQACTEVVMPFCTNGVDDMFEPHSWNLKELSDDCFQQWGVRPRPSWITTMYGGKNISSHTNIVFSNGELDPWSGGGVTKDITDTLVAVTISEGAHHLDLRTKNALDPMSVLLARSLEVRHMKNWIRDFYDSAGKQH

60-90 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.2 μm filter solution of 20 mM NaAc-HAc, 150 mM NaCl, 10% Glycerol, pH4.5.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.