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  4. Calreticulin/CALR Protein, Pig (P.pastoris, His)

Calreticulin/CALR Protein, Pig (P.pastoris, His)

Cat. No.: HY-P71722
Handling Instructions

Calreticulin (CALR) is an essential calcium-binding chaperone involved in ER functions. It interacts with glycoproteins, facilitates NR3C1 nuclear export, regulates maternal gene expression, and aids in oocyte maturation. CALR is released during oocyte activation and prevents polyspermy. It forms an EIF2 complex with CELF1/CUGBP1, CALR3, EIF2S1, EIF2S2, HSP90B1, and HSPA5 and interacts with PDIA3/ERp57, SPACA9, TRIM21, PPIB, PDIA5, and CLCC1. Calreticulin/CALR Protein, Pig (P.pastoris, His) is the recombinant pig-derived Calreticulin/CALR protein, expressed by P. pastoris , with N-His labeled tag. The total length of Calreticulin/CALR Protein, Pig (P.pastoris, His) is 400 a.a., with molecular weight of ~48.6 kDa.

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Description

Calreticulin (CALR) is an essential calcium-binding chaperone involved in ER functions. It interacts with glycoproteins, facilitates NR3C1 nuclear export, regulates maternal gene expression, and aids in oocyte maturation. CALR is released during oocyte activation and prevents polyspermy. It forms an EIF2 complex with CELF1/CUGBP1, CALR3, EIF2S1, EIF2S2, HSP90B1, and HSPA5 and interacts with PDIA3/ERp57, SPACA9, TRIM21, PPIB, PDIA5, and CLCC1. Calreticulin/CALR Protein, Pig (P.pastoris, His) is the recombinant pig-derived Calreticulin/CALR protein, expressed by P. pastoris , with N-His labeled tag. The total length of Calreticulin/CALR Protein, Pig (P.pastoris, His) is 400 a.a., with molecular weight of ~48.6 kDa.
Background

Calreticulin (CALR) is a calcium-binding chaperone that plays a crucial role in the endoplasmic reticulum (ER) by facilitating folding, oligomeric assembly, and quality control through the calreticulin/calnexin cycle. It interacts transiently with almost all monoglucosylated glycoproteins synthesized in the ER. Additionally, CALR interacts with the DNA-binding domain of NR3C1, promoting its nuclear export. CALR is also involved in regulating maternal gene expression and may contribute to oocyte maturation by maintaining calcium homeostasis. In non-activated oocytes, CALR is localized in cortical granules and is released during the cortical reaction upon oocyte activation, potentially preventing polyspermy. CALR exists as a monomer and is part of an EIF2 complex consisting of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1, and HSPA5. It interacts with various proteins such as PDIA3/ERp57, SPACA9, TRIM21, NR3C1, PPIB, PDIA5, and CLCC1.
Species

Pig
Source

P. pastoris
Tag

N-His
Accession

P28491 (18E-417L)
Gene ID

100381266  [NCBI]
Molecular Construction
N-term
His
CALR (18E-417L)
Accession # P28491
C-term
Synonyms
CALRCalreticulin; CRP55; Calregulin; Endoplasmic reticulum resident protein 60; ERp60; HACBP
AA Sequence

EPTIYFKEQFLDGDGWTDRWIESKHKPDFGRFVLSSGKFYGDQEKDKGLQTSQDARFYALSARFEPFSNKGQTLVVQFTVKHEQNIDCGGGYVKLFPDGLDQTDMHGDSEYNIMFGPDICGPGTKKVHVIFNYKGKNVLINKDIRCKDDEFTHLYTLIVRPDNTYEVKIDNSQVESGSLEDDWDFLPPKKIKDPDAVKPEDWDERAKIDDPTDSKPEDWDKPEHIPDPDAKKPEDWDEEMDGEWEPPVIQNPEYKGEWKPRQIDNPDYKGTWIHPEIDNPEYSPDSNIYAYENFAVLGLDLWQVKSGTIFDNFLITNDEAYAEEFGNETWGVTKAAEKQMKDKQDEEQRLKEEEEEKKRKEEEEVDKEDEEDKDEDEEEEDEKEEEEEEDAAAGQAKDEL

Molecular Weight

Approximately 48.6 kDa
Purity

Greater than 90% as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

Calreticulin/CALR Protein, Pig (P.pastoris, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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